Production and comprehensive quality control of recombinant human Interleukin-1β: A case study for a process development strategy

We describe an efficient strategy to produce high-quality proteins by using a single large IMAC chromatography column and enzymatic His-tag removal via the TAGZyme system in pilot scale. Numerous quality assays demonstrated a high purity of the final product, the human cytokine Interleukin-1beta (IL...

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Veröffentlicht in:	Protein expression and purification 2008-02, Vol.57 (2), p.244-254
Hauptverfasser:	Block, Helena, Kubicek, Jan, Labahn, Jörg, Roth, Udo, Schäfer, Frank
Format:	Artikel
Sprache:	eng
Schlagworte:	DAPase Expression screening His-tag cleavage Immobilized-Metal Affinity Chromatography (IMAC) Interleukin-1β (IL-1β) Ni–NTA Protein production TAGZyme X-ray crystallography
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