Antivibrio activity of recombinant lysozyme expressed from black tiger shrimp, Penaeus monodon
The gene coding for lysozyme in black tiger shrimp (Penaeus monodon) was cloned, sequenced and expressed in pEXP5-NT/TOPO vector. The deduced amino acid sequence of P. monodon lysozyme showed 95–98% similarity to that of other shrimp such as Litopenaeus vannamei, Fenneropenaeus chinensis, Marsupenae...
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| Veröffentlicht in: | Aquaculture 2007-11, Vol.272 (1-4), p.246-253 |
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| creator | Tyagi, Anuj Khushiramani, Rekha Karunasagar, Iddya Karunasagar, Indrani |
| description | The gene coding for lysozyme in black tiger shrimp (Penaeus monodon) was cloned, sequenced and expressed in pEXP5-NT/TOPO vector. The deduced amino acid sequence of P. monodon lysozyme showed 95–98% similarity to that of other shrimp such as Litopenaeus vannamei, Fenneropenaeus chinensis, Marsupenaeus japonicus and freshwater prawn Macrobrachium rosenbergii. The lysozyme was purified to homogeneity and observed as a band of approximately 20 kDa in 15% SDS-PAGE. Bacteriolytic activity of lysozyme against different bacterial cultures was determined by solid phase as well as turbiditimetric assay. Lysis was obtained against a broad range of Gram positive and Gram negative bacteria. A gradual decrease in lysozyme activity was observed with respect to time by turbiditimetric assay. Minimal inhibitory concentration (MIC) of purified shrimp lysozyme for Vibrio harveyi was 0.47 μg/ml and for Micrococcus luteus, (MIC) 0.12 μg/ml. The lysozyme was found effective against V. harveyi when tested in sea water with and without EDTA. |
| doi_str_mv | 10.1016/j.aquaculture.2007.08.055 |
| format | Article |
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The deduced amino acid sequence of P. monodon lysozyme showed 95–98% similarity to that of other shrimp such as Litopenaeus vannamei, Fenneropenaeus chinensis, Marsupenaeus japonicus and freshwater prawn Macrobrachium rosenbergii. The lysozyme was purified to homogeneity and observed as a band of approximately 20 kDa in 15% SDS-PAGE. Bacteriolytic activity of lysozyme against different bacterial cultures was determined by solid phase as well as turbiditimetric assay. Lysis was obtained against a broad range of Gram positive and Gram negative bacteria. A gradual decrease in lysozyme activity was observed with respect to time by turbiditimetric assay. Minimal inhibitory concentration (MIC) of purified shrimp lysozyme for Vibrio harveyi was 0.47 μg/ml and for Micrococcus luteus, (MIC) 0.12 μg/ml. The lysozyme was found effective against V. harveyi when tested in sea water with and without EDTA.</description><identifier>ISSN: 0044-8486</identifier><identifier>EISSN: 1873-5622</identifier><identifier>DOI: 10.1016/j.aquaculture.2007.08.055</identifier><identifier>CODEN: AQCLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amino acids ; Animal aquaculture ; Animal productions ; Antibacterial ; antibacterial properties ; Aquaculture ; Bacteria ; Biological and medical sciences ; Cloning ; Enzymes ; Fenneropenaeus chinensis ; Fundamental and applied biological sciences. Psychology ; Gene expression ; General aspects ; Litopenaeus vannamei ; Lysozyme ; Macrobrachium rosenbergii ; Marine ; Marsupenaeus japonicus ; Micrococcus luteus ; molecular cloning ; Penaeus monodon ; Recombinant expression ; recombinant proteins ; seawater ; sequence analysis ; Shellfish ; shrimp ; shrimp culture ; Vibrio harveyi</subject><ispartof>Aquaculture, 2007-11, Vol.272 (1-4), p.246-253</ispartof><rights>2007 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><rights>Copyright Elsevier Sequoia S.A. Nov 26, 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-c2caef2586f1dd7e6404f9af3f297ee35fff6665a9b1d18813a39b83b2e176933</citedby><cites>FETCH-LOGICAL-c499t-c2caef2586f1dd7e6404f9af3f297ee35fff6665a9b1d18813a39b83b2e176933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aquaculture.2007.08.055$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19373054$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Tyagi, Anuj</creatorcontrib><creatorcontrib>Khushiramani, Rekha</creatorcontrib><creatorcontrib>Karunasagar, Iddya</creatorcontrib><creatorcontrib>Karunasagar, Indrani</creatorcontrib><title>Antivibrio activity of recombinant lysozyme expressed from black tiger shrimp, Penaeus monodon</title><title>Aquaculture</title><description>The gene coding for lysozyme in black tiger shrimp (Penaeus monodon) was cloned, sequenced and expressed in pEXP5-NT/TOPO vector. The deduced amino acid sequence of P. monodon lysozyme showed 95–98% similarity to that of other shrimp such as Litopenaeus vannamei, Fenneropenaeus chinensis, Marsupenaeus japonicus and freshwater prawn Macrobrachium rosenbergii. The lysozyme was purified to homogeneity and observed as a band of approximately 20 kDa in 15% SDS-PAGE. Bacteriolytic activity of lysozyme against different bacterial cultures was determined by solid phase as well as turbiditimetric assay. Lysis was obtained against a broad range of Gram positive and Gram negative bacteria. A gradual decrease in lysozyme activity was observed with respect to time by turbiditimetric assay. Minimal inhibitory concentration (MIC) of purified shrimp lysozyme for Vibrio harveyi was 0.47 μg/ml and for Micrococcus luteus, (MIC) 0.12 μg/ml. The lysozyme was found effective against V. harveyi when tested in sea water with and without EDTA.</description><subject>Amino acids</subject><subject>Animal aquaculture</subject><subject>Animal productions</subject><subject>Antibacterial</subject><subject>antibacterial properties</subject><subject>Aquaculture</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Cloning</subject><subject>Enzymes</subject><subject>Fenneropenaeus chinensis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>General aspects</subject><subject>Litopenaeus vannamei</subject><subject>Lysozyme</subject><subject>Macrobrachium rosenbergii</subject><subject>Marine</subject><subject>Marsupenaeus japonicus</subject><subject>Micrococcus luteus</subject><subject>molecular cloning</subject><subject>Penaeus monodon</subject><subject>Recombinant expression</subject><subject>recombinant proteins</subject><subject>seawater</subject><subject>sequence analysis</subject><subject>Shellfish</subject><subject>shrimp</subject><subject>shrimp culture</subject><subject>Vibrio harveyi</subject><issn>0044-8486</issn><issn>1873-5622</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqNkcFu1DAURSMEEkPhGzBIsGqGZztx7GU1KhSpEkjQLZbjPBcPiT21k4rh63E0lUCsWPktjq-vz6uqVxS2FKh4t9-au8XYZZyXhFsG0G1BbqFtH1UbKjtet4Kxx9UGoGlq2UjxtHqW8x4AhGjppvp2EWZ_7_vkIzF2HecjiY4ktHHqfTBhJuMxx1_HCQn-PCTMGQfiUpxIPxr7g8z-FhPJ35OfDufkMwaDSyZTDHGI4Xn1xJkx44uH86y6eX_5dXdVX3_68HF3cV3bRqm5tswadKyVwtFh6FA00DhlHHdMdYi8dc6JUtiong5USsoNV73kPUPaCcX5WfX2lHtI8W7BPOvJZ4vjaALGJWuqpBIKaAFf_wPu45JC6aYZNJ2UnVwhdYJsijkndPpQfmfSUVPQq3a9139p16t2DVIX7eXum4cHTLZmdMkE6_OfAMU7Dm1TuJcnzpmozW0qzM0XVhoCSC4ZXZN2JwKLuHuPSWfrMVgcfNnOrIfo_6PPbwZoqac</recordid><startdate>20071126</startdate><enddate>20071126</enddate><creator>Tyagi, Anuj</creator><creator>Khushiramani, Rekha</creator><creator>Karunasagar, Iddya</creator><creator>Karunasagar, Indrani</creator><general>Elsevier B.V</general><general>Elsevier Science</general><general>Elsevier Sequoia S.A</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7ST</scope><scope>7TN</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7TM</scope></search><sort><creationdate>20071126</creationdate><title>Antivibrio activity of recombinant lysozyme expressed from black tiger shrimp, Penaeus monodon</title><author>Tyagi, Anuj ; Khushiramani, Rekha ; Karunasagar, Iddya ; Karunasagar, Indrani</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-c2caef2586f1dd7e6404f9af3f297ee35fff6665a9b1d18813a39b83b2e176933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amino acids</topic><topic>Animal aquaculture</topic><topic>Animal productions</topic><topic>Antibacterial</topic><topic>antibacterial properties</topic><topic>Aquaculture</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Cloning</topic><topic>Enzymes</topic><topic>Fenneropenaeus chinensis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>General aspects</topic><topic>Litopenaeus vannamei</topic><topic>Lysozyme</topic><topic>Macrobrachium rosenbergii</topic><topic>Marine</topic><topic>Marsupenaeus japonicus</topic><topic>Micrococcus luteus</topic><topic>molecular cloning</topic><topic>Penaeus monodon</topic><topic>Recombinant expression</topic><topic>recombinant proteins</topic><topic>seawater</topic><topic>sequence analysis</topic><topic>Shellfish</topic><topic>shrimp</topic><topic>shrimp culture</topic><topic>Vibrio harveyi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tyagi, Anuj</creatorcontrib><creatorcontrib>Khushiramani, Rekha</creatorcontrib><creatorcontrib>Karunasagar, Iddya</creatorcontrib><creatorcontrib>Karunasagar, Indrani</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Aquaculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tyagi, Anuj</au><au>Khushiramani, Rekha</au><au>Karunasagar, Iddya</au><au>Karunasagar, Indrani</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antivibrio activity of recombinant lysozyme expressed from black tiger shrimp, Penaeus monodon</atitle><jtitle>Aquaculture</jtitle><date>2007-11-26</date><risdate>2007</risdate><volume>272</volume><issue>1-4</issue><spage>246</spage><epage>253</epage><pages>246-253</pages><issn>0044-8486</issn><eissn>1873-5622</eissn><coden>AQCLAL</coden><abstract>The gene coding for lysozyme in black tiger shrimp (Penaeus monodon) was cloned, sequenced and expressed in pEXP5-NT/TOPO vector. The deduced amino acid sequence of P. monodon lysozyme showed 95–98% similarity to that of other shrimp such as Litopenaeus vannamei, Fenneropenaeus chinensis, Marsupenaeus japonicus and freshwater prawn Macrobrachium rosenbergii. The lysozyme was purified to homogeneity and observed as a band of approximately 20 kDa in 15% SDS-PAGE. Bacteriolytic activity of lysozyme against different bacterial cultures was determined by solid phase as well as turbiditimetric assay. Lysis was obtained against a broad range of Gram positive and Gram negative bacteria. A gradual decrease in lysozyme activity was observed with respect to time by turbiditimetric assay. Minimal inhibitory concentration (MIC) of purified shrimp lysozyme for Vibrio harveyi was 0.47 μg/ml and for Micrococcus luteus, (MIC) 0.12 μg/ml. The lysozyme was found effective against V. harveyi when tested in sea water with and without EDTA.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.aquaculture.2007.08.055</doi><tpages>8</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Amino acids Animal aquaculture Animal productions Antibacterial antibacterial properties Aquaculture Bacteria Biological and medical sciences Cloning Enzymes Fenneropenaeus chinensis Fundamental and applied biological sciences. Psychology Gene expression General aspects Litopenaeus vannamei Lysozyme Macrobrachium rosenbergii Marine Marsupenaeus japonicus Micrococcus luteus molecular cloning Penaeus monodon Recombinant expression recombinant proteins seawater sequence analysis Shellfish shrimp shrimp culture Vibrio harveyi |
| title | Antivibrio activity of recombinant lysozyme expressed from black tiger shrimp, Penaeus monodon |
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