Detection of Escherichia coli in ready‐to‐eat fresh vegetables using broad‐host‐range recombinant phages

Aim To construct a simple method to detect Escherichia coli in ready‐to‐eat fresh vegetables using broad‐host‐range recombinant phages. Methods and Results Firstly, a gene encoding cytochrome c peroxidase (CCP) chromogenic enzyme was inserted into genomes of wild‐type phages IP008 and IP052 to produ...

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Veröffentlicht in:	Journal of applied microbiology 2018-06, Vol.124 (6), p.1610-1616
Hauptverfasser:	Hoang, H.A., Quy, N.T.C., Chi, N.V.T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteriophages - genetics broad‐host‐range phage Colorimetry Cytochrome c detection DNA, Recombinant - genetics E coli Enzymes Escherichia coli Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli - physiology faecal contamination indicator Feces Food contamination Genomes Host Specificity - genetics Methods Mustard Peroxidase Phages Vegetables Vegetables - microbiology
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