A rapid, ratiometric, enzyme-free, and sensitive single-step miRNA detection using three-way junction based FRET probes
MicroRNAs (miRNAs) are single stranded endogenous molecules composed of only 18-24 nucleotides which are critical for gene expression regulating the translation of messenger RNAs. Conventional methods based on enzyme-assisted nucleic acid amplification techniques have many problems, such as easy con...
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| Veröffentlicht in: | Nanotechnology 2018-03, Vol.29 (11), p.114001-114001 |
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| container_issue | 11 |
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| container_title | Nanotechnology |
| container_volume | 29 |
| creator | Luo, Qingying Liu, Lin Yang, Cai Yuan, Jing Feng, Hongtao Chen, Yan Zhao, Peng Yu, Zhiqiang Jin, Zongwen |
| description | MicroRNAs (miRNAs) are single stranded endogenous molecules composed of only 18-24 nucleotides which are critical for gene expression regulating the translation of messenger RNAs. Conventional methods based on enzyme-assisted nucleic acid amplification techniques have many problems, such as easy contamination, high cost, susceptibility to false amplification, and tendency to have sequence mismatches. Here we report a rapid, ratiometric, enzyme-free, sensitive, and highly selective single-step miRNA detection using three-way junction assembled (or self-assembled) FRET probes. The developed strategy can be operated within the linear range from subnanomolar to hundred nanomolar concentrations of miRNAs. In comparison with the traditional approaches, our method showed high sensitivity for the miRNA detection and extreme selectivity for the efficient discrimination of single-base mismatches. The results reveal that the strategy paved a new avenue for the design of novel highly specific probes applicable in diagnostics and potentially in microscopic imaging of miRNAs in real biological environments. |
| doi_str_mv | 10.1088/1361-6528/aaa824 |
| format | Article |
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The results reveal that the strategy paved a new avenue for the design of novel highly specific probes applicable in diagnostics and potentially in microscopic imaging of miRNAs in real biological environments.</description><identifier>ISSN: 0957-4484</identifier><identifier>EISSN: 1361-6528</identifier><identifier>DOI: 10.1088/1361-6528/aaa824</identifier><identifier>PMID: 29337292</identifier><identifier>CODEN: NNOTER</identifier><language>eng</language><publisher>England: IOP Publishing</publisher><subject>Calibration ; Fluorescence Resonance Energy Transfer - methods ; fluorescent biosensor ; FRET ; MicroRNAs - analysis ; miRNA detection ; ratiometric</subject><ispartof>Nanotechnology, 2018-03, Vol.29 (11), p.114001-114001</ispartof><rights>2018 IOP Publishing Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-dd2654b85bd89497ee082afaf9a1c3243a92b70787058158e150e9859364120a3</citedby><cites>FETCH-LOGICAL-c370t-dd2654b85bd89497ee082afaf9a1c3243a92b70787058158e150e9859364120a3</cites><orcidid>0000-0003-1270-3400 ; 0000-0002-7108-725X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1088/1361-6528/aaa824/pdf$$EPDF$$P50$$Giop$$H</linktopdf><link.rule.ids>314,780,784,27923,27924,53845,53892</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29337292$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luo, Qingying</creatorcontrib><creatorcontrib>Liu, Lin</creatorcontrib><creatorcontrib>Yang, Cai</creatorcontrib><creatorcontrib>Yuan, Jing</creatorcontrib><creatorcontrib>Feng, Hongtao</creatorcontrib><creatorcontrib>Chen, Yan</creatorcontrib><creatorcontrib>Zhao, Peng</creatorcontrib><creatorcontrib>Yu, Zhiqiang</creatorcontrib><creatorcontrib>Jin, Zongwen</creatorcontrib><title>A rapid, ratiometric, enzyme-free, and sensitive single-step miRNA detection using three-way junction based FRET probes</title><title>Nanotechnology</title><addtitle>NANO</addtitle><addtitle>Nanotechnology</addtitle><description>MicroRNAs (miRNAs) are single stranded endogenous molecules composed of only 18-24 nucleotides which are critical for gene expression regulating the translation of messenger RNAs. Conventional methods based on enzyme-assisted nucleic acid amplification techniques have many problems, such as easy contamination, high cost, susceptibility to false amplification, and tendency to have sequence mismatches. Here we report a rapid, ratiometric, enzyme-free, sensitive, and highly selective single-step miRNA detection using three-way junction assembled (or self-assembled) FRET probes. The developed strategy can be operated within the linear range from subnanomolar to hundred nanomolar concentrations of miRNAs. In comparison with the traditional approaches, our method showed high sensitivity for the miRNA detection and extreme selectivity for the efficient discrimination of single-base mismatches. The results reveal that the strategy paved a new avenue for the design of novel highly specific probes applicable in diagnostics and potentially in microscopic imaging of miRNAs in real biological environments.</description><subject>Calibration</subject><subject>Fluorescence Resonance Energy Transfer - methods</subject><subject>fluorescent biosensor</subject><subject>FRET</subject><subject>MicroRNAs - analysis</subject><subject>miRNA detection</subject><subject>ratiometric</subject><issn>0957-4484</issn><issn>1361-6528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kd1r2zAUxcXYWNKP9z4VPW4QL_qyLT2G0HaFskHInoVsXbcKsexJdkv610_BWZ7G4MKFe885F_2E0A0l3yiRckl5QbMiZ3JpjJFMfEDz8-gjmhOVl5kQUszQRYw7QiiVjH5GM6Y4L5lic_S2wsH0zi5SG1zXwhBcvcDg3w8tZE0AWGDjLY7goxvcK-Do_PMesjhAj1u3-bHCFgaok9nj8bjEw0uyZW_mgHejnxaViWDx_eZui_vQVRCv0KfG7CNcn_ol-nV_t11_z55-PjyuV09ZzUsyZNayIheVzCsrlVAlAJHMNKZRhtacCW4Uq0pSypLkkuYSaE5AyVzxQlBGDL9EX6bcdPb3CHHQrYs17PfGQzdGTZVUReLEWZKSSVqHLsYAje6Da004aEr0Ebc-stVHtnrCnSy3p_SxasGeDX_5JsFiEriu17tuDD499n95X_8h98Z3KVJTmkqkX9S9bfgf77aWRQ</recordid><startdate>20180316</startdate><enddate>20180316</enddate><creator>Luo, Qingying</creator><creator>Liu, Lin</creator><creator>Yang, Cai</creator><creator>Yuan, Jing</creator><creator>Feng, Hongtao</creator><creator>Chen, Yan</creator><creator>Zhao, Peng</creator><creator>Yu, Zhiqiang</creator><creator>Jin, Zongwen</creator><general>IOP Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1270-3400</orcidid><orcidid>https://orcid.org/0000-0002-7108-725X</orcidid></search><sort><creationdate>20180316</creationdate><title>A rapid, ratiometric, enzyme-free, and sensitive single-step miRNA detection using three-way junction based FRET probes</title><author>Luo, Qingying ; Liu, Lin ; Yang, Cai ; Yuan, Jing ; Feng, Hongtao ; Chen, Yan ; Zhao, Peng ; Yu, Zhiqiang ; Jin, Zongwen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-dd2654b85bd89497ee082afaf9a1c3243a92b70787058158e150e9859364120a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Calibration</topic><topic>Fluorescence Resonance Energy Transfer - methods</topic><topic>fluorescent biosensor</topic><topic>FRET</topic><topic>MicroRNAs - analysis</topic><topic>miRNA detection</topic><topic>ratiometric</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Qingying</creatorcontrib><creatorcontrib>Liu, Lin</creatorcontrib><creatorcontrib>Yang, Cai</creatorcontrib><creatorcontrib>Yuan, Jing</creatorcontrib><creatorcontrib>Feng, Hongtao</creatorcontrib><creatorcontrib>Chen, Yan</creatorcontrib><creatorcontrib>Zhao, Peng</creatorcontrib><creatorcontrib>Yu, Zhiqiang</creatorcontrib><creatorcontrib>Jin, Zongwen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nanotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Qingying</au><au>Liu, Lin</au><au>Yang, Cai</au><au>Yuan, Jing</au><au>Feng, Hongtao</au><au>Chen, Yan</au><au>Zhao, Peng</au><au>Yu, Zhiqiang</au><au>Jin, Zongwen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid, ratiometric, enzyme-free, and sensitive single-step miRNA detection using three-way junction based FRET probes</atitle><jtitle>Nanotechnology</jtitle><stitle>NANO</stitle><addtitle>Nanotechnology</addtitle><date>2018-03-16</date><risdate>2018</risdate><volume>29</volume><issue>11</issue><spage>114001</spage><epage>114001</epage><pages>114001-114001</pages><issn>0957-4484</issn><eissn>1361-6528</eissn><coden>NNOTER</coden><abstract>MicroRNAs (miRNAs) are single stranded endogenous molecules composed of only 18-24 nucleotides which are critical for gene expression regulating the translation of messenger RNAs. Conventional methods based on enzyme-assisted nucleic acid amplification techniques have many problems, such as easy contamination, high cost, susceptibility to false amplification, and tendency to have sequence mismatches. Here we report a rapid, ratiometric, enzyme-free, sensitive, and highly selective single-step miRNA detection using three-way junction assembled (or self-assembled) FRET probes. The developed strategy can be operated within the linear range from subnanomolar to hundred nanomolar concentrations of miRNAs. In comparison with the traditional approaches, our method showed high sensitivity for the miRNA detection and extreme selectivity for the efficient discrimination of single-base mismatches. 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| source | MEDLINE; IOP Publishing Journals; Institute of Physics (IOP) Journals - HEAL-Link |
| subjects | Calibration Fluorescence Resonance Energy Transfer - methods fluorescent biosensor FRET MicroRNAs - analysis miRNA detection ratiometric |
| title | A rapid, ratiometric, enzyme-free, and sensitive single-step miRNA detection using three-way junction based FRET probes |
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