Effect of bone morphogenetic proteins 2 and 4 on survival and development of bovine secondary follicles cultured in vitro
This study evaluated the effect of bone morphogenetic proteins 2 (BMP2) and 4 (BMP2) on follicle development and mRNA expression for GDF9, Cyclin B1, BMPR1A, BMPR1B, BMPRII, FSHR and SMAD1 in bovine secondary follicles cultured in vitro. Isolated secondary follicles were cultured for 18 days in TCM1...
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| Veröffentlicht in: | Theriogenology 2018-04, Vol.110, p.44-51 |
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| creator | da Cunha, E.V. Melo, L.R.F. Sousa, G.B. Araújo, V.R. Vasconcelos, G.L. Silva, A.W.B. Silva, J.R.V. |
| description | This study evaluated the effect of bone morphogenetic proteins 2 (BMP2) and 4 (BMP2) on follicle development and mRNA expression for GDF9, Cyclin B1, BMPR1A, BMPR1B, BMPRII, FSHR and SMAD1 in bovine secondary follicles cultured in vitro. Isolated secondary follicles were cultured for 18 days in TCM199+ medium alone or supplemented with BMP2 (10 ng/mL), BMP4 (100 ng/mL) or combination of both BMP2 and 4. Real-time PCR was used to analyze mRNA levels in fresh and cultured follicles. After 18 days of culture, follicles cultured with BMP2 alone or with BMP4 alone had larger diameters when compared to control (P |
| doi_str_mv | 10.1016/j.theriogenology.2017.12.032 |
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•BMP2 and BMP4 increase follicular diameter after 18 days of in vitro culture.•The interaction of BMP2 and BMP4 proteins did not increase follicular diameter.•BMP2 and BMP4 do not influence the relative mRNA expression for GDF9, Cyclin B1, BMPIA, BMPRIB, BMPRII, FSHR and SMAD1 genes.•In vitro culture induces a down-regulation for mRNA expression of BMPR1A.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2017.12.032</identifier><identifier>PMID: 29331831</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antrum ; Bone Morphogenetic Protein 2 - pharmacology ; Bone Morphogenetic Protein 4 - pharmacology ; Bone Morphogenetic Protein Receptors - genetics ; Bone Morphogenetic Protein Receptors - metabolism ; Cattle ; Cell Survival - drug effects ; Cell Survival - genetics ; Cells, Cultured ; Expression ; Female ; Gene Expression Regulation - drug effects ; Oocytes - drug effects ; Oocytes - physiology ; Oogenesis - drug effects ; Oogenesis - genetics ; Ovarian Follicle - drug effects ; Ovarian Follicle - physiology ; Ovary ; Preantral ; Viability</subject><ispartof>Theriogenology, 2018-04, Vol.110, p.44-51</ispartof><rights>2017</rights><rights>Copyright © 2017. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-446f6e25f4fb76fb75342c4734e704d37fcfd19777555cd8c660dc7369613c373</citedby><cites>FETCH-LOGICAL-c452t-446f6e25f4fb76fb75342c4734e704d37fcfd19777555cd8c660dc7369613c373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0093691X17306222$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29331831$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Cunha, E.V.</creatorcontrib><creatorcontrib>Melo, L.R.F.</creatorcontrib><creatorcontrib>Sousa, G.B.</creatorcontrib><creatorcontrib>Araújo, V.R.</creatorcontrib><creatorcontrib>Vasconcelos, G.L.</creatorcontrib><creatorcontrib>Silva, A.W.B.</creatorcontrib><creatorcontrib>Silva, J.R.V.</creatorcontrib><title>Effect of bone morphogenetic proteins 2 and 4 on survival and development of bovine secondary follicles cultured in vitro</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>This study evaluated the effect of bone morphogenetic proteins 2 (BMP2) and 4 (BMP2) on follicle development and mRNA expression for GDF9, Cyclin B1, BMPR1A, BMPR1B, BMPRII, FSHR and SMAD1 in bovine secondary follicles cultured in vitro. Isolated secondary follicles were cultured for 18 days in TCM199+ medium alone or supplemented with BMP2 (10 ng/mL), BMP4 (100 ng/mL) or combination of both BMP2 and 4. Real-time PCR was used to analyze mRNA levels in fresh and cultured follicles. After 18 days of culture, follicles cultured with BMP2 alone or with BMP4 alone had larger diameters when compared to control (P < .05). In addition, all treatments promoted antrum formation and maintained a high viability rate through the growing period. The presence of BMP2, BMP4 or both together did not influence mRNA expression for the tested genes. However, the in vitro culture induces down-regulation for mRNA expression of BMPR1A. In conclusion, the addition of BMP2 or BMP4 alone in cultured medium promotes follicular growth and antrum formation in bovine follicles after 18 days of in vitro culture.
•BMP2 and BMP4 increase follicular diameter after 18 days of in vitro culture.•The interaction of BMP2 and BMP4 proteins did not increase follicular diameter.•BMP2 and BMP4 do not influence the relative mRNA expression for GDF9, Cyclin B1, BMPIA, BMPRIB, BMPRII, FSHR and SMAD1 genes.•In vitro culture induces a down-regulation for mRNA expression of BMPR1A.</description><subject>Animals</subject><subject>Antrum</subject><subject>Bone Morphogenetic Protein 2 - pharmacology</subject><subject>Bone Morphogenetic Protein 4 - pharmacology</subject><subject>Bone Morphogenetic Protein Receptors - genetics</subject><subject>Bone Morphogenetic Protein Receptors - metabolism</subject><subject>Cattle</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - genetics</subject><subject>Cells, Cultured</subject><subject>Expression</subject><subject>Female</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - physiology</subject><subject>Oogenesis - drug effects</subject><subject>Oogenesis - genetics</subject><subject>Ovarian Follicle - drug effects</subject><subject>Ovarian Follicle - physiology</subject><subject>Ovary</subject><subject>Preantral</subject><subject>Viability</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-KFDEQxoO4uOPqK0gOHrx0mz_dSQe8yLKrwsJedsFb6EkquxnSyZh0N6xP47P4ZGacUfDmoSgovvqK71cIvaWkpYSK97t2foTs0wPEFNLDU8sIlS1lLeHsGdrQQaqGM06fow0hijdC0a_n6GUpO0IIF4K-QOdMcU4HTjfo-5VzYGacHN6mCHhKef948IbZG7zPaQYfC2Z4jBZ3OEVclrz6dQy_JxZWCGk_QTxZrL6aFDAp2jE_YZdC8CZAwWYJ85LBYh9__lj9nNMrdObGUOD1qV-g--uru8vPzc3tpy-XH28a0_VsbrpOOAGsd53bSlGr5x0zneQdSNJZLp1xliopZd_3xg5GCGKN5EIJyg2X_AK9O_rWNN8WKLOefDEQwhghLUVTNah-UANXVfrhKDU5lZLB6X32Uw2iKdEH-nqn_6WvD_Q1ZbrSr-tvTpeW7QT27_If3FVwfRRAzbt6yLoYD9GA9bl-Qdvk_-_SLy2Eoao</recordid><startdate>20180401</startdate><enddate>20180401</enddate><creator>da Cunha, E.V.</creator><creator>Melo, L.R.F.</creator><creator>Sousa, G.B.</creator><creator>Araújo, V.R.</creator><creator>Vasconcelos, G.L.</creator><creator>Silva, A.W.B.</creator><creator>Silva, J.R.V.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180401</creationdate><title>Effect of bone morphogenetic proteins 2 and 4 on survival and development of bovine secondary follicles cultured in vitro</title><author>da Cunha, E.V. ; Melo, L.R.F. ; Sousa, G.B. ; Araújo, V.R. ; Vasconcelos, G.L. ; Silva, A.W.B. ; Silva, J.R.V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-446f6e25f4fb76fb75342c4734e704d37fcfd19777555cd8c660dc7369613c373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Antrum</topic><topic>Bone Morphogenetic Protein 2 - pharmacology</topic><topic>Bone Morphogenetic Protein 4 - pharmacology</topic><topic>Bone Morphogenetic Protein Receptors - genetics</topic><topic>Bone Morphogenetic Protein Receptors - metabolism</topic><topic>Cattle</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - genetics</topic><topic>Cells, Cultured</topic><topic>Expression</topic><topic>Female</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - physiology</topic><topic>Oogenesis - drug effects</topic><topic>Oogenesis - genetics</topic><topic>Ovarian Follicle - drug effects</topic><topic>Ovarian Follicle - physiology</topic><topic>Ovary</topic><topic>Preantral</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>da Cunha, E.V.</creatorcontrib><creatorcontrib>Melo, L.R.F.</creatorcontrib><creatorcontrib>Sousa, G.B.</creatorcontrib><creatorcontrib>Araújo, V.R.</creatorcontrib><creatorcontrib>Vasconcelos, G.L.</creatorcontrib><creatorcontrib>Silva, A.W.B.</creatorcontrib><creatorcontrib>Silva, J.R.V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>da Cunha, E.V.</au><au>Melo, L.R.F.</au><au>Sousa, G.B.</au><au>Araújo, V.R.</au><au>Vasconcelos, G.L.</au><au>Silva, A.W.B.</au><au>Silva, J.R.V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of bone morphogenetic proteins 2 and 4 on survival and development of bovine secondary follicles cultured in vitro</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2018-04-01</date><risdate>2018</risdate><volume>110</volume><spage>44</spage><epage>51</epage><pages>44-51</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>This study evaluated the effect of bone morphogenetic proteins 2 (BMP2) and 4 (BMP2) on follicle development and mRNA expression for GDF9, Cyclin B1, BMPR1A, BMPR1B, BMPRII, FSHR and SMAD1 in bovine secondary follicles cultured in vitro. Isolated secondary follicles were cultured for 18 days in TCM199+ medium alone or supplemented with BMP2 (10 ng/mL), BMP4 (100 ng/mL) or combination of both BMP2 and 4. Real-time PCR was used to analyze mRNA levels in fresh and cultured follicles. After 18 days of culture, follicles cultured with BMP2 alone or with BMP4 alone had larger diameters when compared to control (P < .05). In addition, all treatments promoted antrum formation and maintained a high viability rate through the growing period. The presence of BMP2, BMP4 or both together did not influence mRNA expression for the tested genes. However, the in vitro culture induces down-regulation for mRNA expression of BMPR1A. In conclusion, the addition of BMP2 or BMP4 alone in cultured medium promotes follicular growth and antrum formation in bovine follicles after 18 days of in vitro culture.
•BMP2 and BMP4 increase follicular diameter after 18 days of in vitro culture.•The interaction of BMP2 and BMP4 proteins did not increase follicular diameter.•BMP2 and BMP4 do not influence the relative mRNA expression for GDF9, Cyclin B1, BMPIA, BMPRIB, BMPRII, FSHR and SMAD1 genes.•In vitro culture induces a down-regulation for mRNA expression of BMPR1A.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>29331831</pmid><doi>10.1016/j.theriogenology.2017.12.032</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Antrum Bone Morphogenetic Protein 2 - pharmacology Bone Morphogenetic Protein 4 - pharmacology Bone Morphogenetic Protein Receptors - genetics Bone Morphogenetic Protein Receptors - metabolism Cattle Cell Survival - drug effects Cell Survival - genetics Cells, Cultured Expression Female Gene Expression Regulation - drug effects Oocytes - drug effects Oocytes - physiology Oogenesis - drug effects Oogenesis - genetics Ovarian Follicle - drug effects Ovarian Follicle - physiology Ovary Preantral Viability |
| title | Effect of bone morphogenetic proteins 2 and 4 on survival and development of bovine secondary follicles cultured in vitro |
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