Short-term and long-term effects of osteoporosis on incisor teeth and femoral bones evaluated by Raman spectroscopy and energy dispersive X-ray analysis in ovariectomized rats
There are few published data on the relationship between loss of bone mass due to osteoporosis and poor tooth quality. This study analyzed the effects of osteoporosis on incisor teeth and femoral bones using optical techniques in rats. Twenty female Wistar rats aged 6 months ( n = 20) were randomiz...
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| Veröffentlicht in: | Journal of bone and mineral metabolism 2019-01, Vol.37 (1), p.18-27 |
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| creator | Paolillo, Fernanda Rossi Romano, Renan Arnon de Matos, Luciana Martin, Airton Abrahão Guimarães, Francisco Eduardo Gontijo de Castro Neto, Jarbas Caiado Bagnato, Vanderlei Salvador |
| description | There are few published data on the relationship between loss of bone mass due to osteoporosis and poor tooth quality. This study analyzed the effects of osteoporosis on incisor teeth and femoral bones using optical techniques in rats. Twenty female Wistar rats aged 6 months (
n
= 20) were randomized into two groups: control group, non-ovariectomized rats (
n
= 10); ovariectomy group, ovariectomized rats to induce osteoporosis (
n
= 10). Each group was subdivided randomly into two groups containing five rats each as follows. Control group 1: non-ovariectomized rats euthanized at the age of 9 or 3 months post-ovariectomy (
n
= 5); Control group 2: non-ovariectomized rats euthanized at the age of 1 year or 6 months post-ovariectomy (
n
= 5); ovariectomy group 1: ovariectomized rats euthanized at the age of 9 months or 3 months post-ovariectomy (
n
= 5); ovariectomy group 2: ovariectomized rats euthanized at the age of 1 year or 6 months post-ovariectomy (
n
= 5). The incisor teeth and femoral bones of Wistar rats were removed to perform Raman spectroscopy using an excitation laser at 785 nm. In addition, an energy-dispersive X-ray spectrometer system was used to evaluate calcium (Ca) and phosphorus (P). The main findings included significant changes (
p
|
| doi_str_mv | 10.1007/s00774-018-0903-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1989588783</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1993066202</sourcerecordid><originalsourceid>FETCH-LOGICAL-c462t-d44ceb8bbc8ee42da919b61283929a28e506bbc7f29e5c402a21197047ad7ce03</originalsourceid><addsrcrecordid>eNp1kd2K1TAUhYMoznH0AbyRgDfeRJM0bZJLGfwZGBD8Ae9Kmu6eydAmNUkP1JfyFU2nMyKCNwmb9a21SRZCzxl9zSiVb1I5pCCUKUI1rUjzAB2YqGpSN1Q8RAeqmSBKSn2GnqR0QymTtWSP0RnXlRCK8QP69eU6xEwyxAkb3-Mx-OM-wTCAzQmHAYeUIcwhhuTK7LHz1qUQcQbI17e2AaYQzYi74CFhOJlxMRl63K34s5mMx2kuYSXAhnm9dYCHeFxx74oSkzsB_k6i2TQzrtse53E4meiKL0zuZwmLJqen6NFgxgTP7u5z9O39u68XH8nVpw-XF2-viBUNz6QXwkKnus4qAMF7o5nuGsZVpbk2XEFNmyLKgWuoraDccMa0pEKaXlqg1Tl6tefOMfxYIOV2csnCOBoPYUkt00rXSklVFfTlP-hNWGJ5xkbpijYNp7xQbKds-YUUYWjn6CYT15bRdmuz3dtsS5vt1mbbFM-Lu-Slm6D_47ivrwB8B1KR_BHiX6v_m_obEmyuRg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1993066202</pqid></control><display><type>article</type><title>Short-term and long-term effects of osteoporosis on incisor teeth and femoral bones evaluated by Raman spectroscopy and energy dispersive X-ray analysis in ovariectomized rats</title><source>SpringerLink (Online service)</source><creator>Paolillo, Fernanda Rossi ; Romano, Renan Arnon ; de Matos, Luciana ; Martin, Airton Abrahão ; Guimarães, Francisco Eduardo Gontijo ; de Castro Neto, Jarbas Caiado ; Bagnato, Vanderlei Salvador</creator><creatorcontrib>Paolillo, Fernanda Rossi ; Romano, Renan Arnon ; de Matos, Luciana ; Martin, Airton Abrahão ; Guimarães, Francisco Eduardo Gontijo ; de Castro Neto, Jarbas Caiado ; Bagnato, Vanderlei Salvador</creatorcontrib><description>There are few published data on the relationship between loss of bone mass due to osteoporosis and poor tooth quality. This study analyzed the effects of osteoporosis on incisor teeth and femoral bones using optical techniques in rats. Twenty female Wistar rats aged 6 months (
n
= 20) were randomized into two groups: control group, non-ovariectomized rats (
n
= 10); ovariectomy group, ovariectomized rats to induce osteoporosis (
n
= 10). Each group was subdivided randomly into two groups containing five rats each as follows. Control group 1: non-ovariectomized rats euthanized at the age of 9 or 3 months post-ovariectomy (
n
= 5); Control group 2: non-ovariectomized rats euthanized at the age of 1 year or 6 months post-ovariectomy (
n
= 5); ovariectomy group 1: ovariectomized rats euthanized at the age of 9 months or 3 months post-ovariectomy (
n
= 5); ovariectomy group 2: ovariectomized rats euthanized at the age of 1 year or 6 months post-ovariectomy (
n
= 5). The incisor teeth and femoral bones of Wistar rats were removed to perform Raman spectroscopy using an excitation laser at 785 nm. In addition, an energy-dispersive X-ray spectrometer system was used to evaluate calcium (Ca) and phosphorus (P). The main findings included significant changes (
p
< 0.05) for phosphate and carbonate band areas for both incisor teeth and femur bones. In addition, there was significant negative correlation between the P concentration and phosphate/carbonate ratio (lower P content–larger ratio,
p
< 0.05) for incisor teeth and femoral bones. The proline and CH
2
wag band areas were significantly reduced only for the incisor teeth (
p
< 0.05). Therefore, Raman spectroscopy assessed the compositional, physicochemical and structural changes in hard tissue. The current study also pointed out the possible action mechanisms of these changes, bone fracture risk and dental fragility. It is important to emphasize that poor dental quality may also occur due to osteoporosis.</description><identifier>ISSN: 0914-8779</identifier><identifier>EISSN: 1435-5604</identifier><identifier>DOI: 10.1007/s00774-018-0903-6</identifier><identifier>PMID: 29344812</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Age ; Bone mass ; Bones ; Calcium ; Femur ; Long-term effects ; Medicine ; Medicine & Public Health ; Metabolic Diseases ; Original Article ; Orthopedics ; Osteoporosis ; Ovariectomy ; Phosphorus ; Proline ; Raman spectroscopy ; Rodents ; Spectrum analysis ; Teeth</subject><ispartof>Journal of bone and mineral metabolism, 2019-01, Vol.37 (1), p.18-27</ispartof><rights>The Japanese Society for Bone and Mineral Research and Springer Japan KK, part of Springer Nature 2018</rights><rights>Journal of Bone and Mineral Metabolism is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-d44ceb8bbc8ee42da919b61283929a28e506bbc7f29e5c402a21197047ad7ce03</citedby><cites>FETCH-LOGICAL-c462t-d44ceb8bbc8ee42da919b61283929a28e506bbc7f29e5c402a21197047ad7ce03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00774-018-0903-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00774-018-0903-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29344812$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paolillo, Fernanda Rossi</creatorcontrib><creatorcontrib>Romano, Renan Arnon</creatorcontrib><creatorcontrib>de Matos, Luciana</creatorcontrib><creatorcontrib>Martin, Airton Abrahão</creatorcontrib><creatorcontrib>Guimarães, Francisco Eduardo Gontijo</creatorcontrib><creatorcontrib>de Castro Neto, Jarbas Caiado</creatorcontrib><creatorcontrib>Bagnato, Vanderlei Salvador</creatorcontrib><title>Short-term and long-term effects of osteoporosis on incisor teeth and femoral bones evaluated by Raman spectroscopy and energy dispersive X-ray analysis in ovariectomized rats</title><title>Journal of bone and mineral metabolism</title><addtitle>J Bone Miner Metab</addtitle><addtitle>J Bone Miner Metab</addtitle><description>There are few published data on the relationship between loss of bone mass due to osteoporosis and poor tooth quality. This study analyzed the effects of osteoporosis on incisor teeth and femoral bones using optical techniques in rats. Twenty female Wistar rats aged 6 months (
n
= 20) were randomized into two groups: control group, non-ovariectomized rats (
n
= 10); ovariectomy group, ovariectomized rats to induce osteoporosis (
n
= 10). Each group was subdivided randomly into two groups containing five rats each as follows. Control group 1: non-ovariectomized rats euthanized at the age of 9 or 3 months post-ovariectomy (
n
= 5); Control group 2: non-ovariectomized rats euthanized at the age of 1 year or 6 months post-ovariectomy (
n
= 5); ovariectomy group 1: ovariectomized rats euthanized at the age of 9 months or 3 months post-ovariectomy (
n
= 5); ovariectomy group 2: ovariectomized rats euthanized at the age of 1 year or 6 months post-ovariectomy (
n
= 5). The incisor teeth and femoral bones of Wistar rats were removed to perform Raman spectroscopy using an excitation laser at 785 nm. In addition, an energy-dispersive X-ray spectrometer system was used to evaluate calcium (Ca) and phosphorus (P). The main findings included significant changes (
p
< 0.05) for phosphate and carbonate band areas for both incisor teeth and femur bones. In addition, there was significant negative correlation between the P concentration and phosphate/carbonate ratio (lower P content–larger ratio,
p
< 0.05) for incisor teeth and femoral bones. The proline and CH
2
wag band areas were significantly reduced only for the incisor teeth (
p
< 0.05). Therefore, Raman spectroscopy assessed the compositional, physicochemical and structural changes in hard tissue. The current study also pointed out the possible action mechanisms of these changes, bone fracture risk and dental fragility. It is important to emphasize that poor dental quality may also occur due to osteoporosis.</description><subject>Age</subject><subject>Bone mass</subject><subject>Bones</subject><subject>Calcium</subject><subject>Femur</subject><subject>Long-term effects</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metabolic Diseases</subject><subject>Original Article</subject><subject>Orthopedics</subject><subject>Osteoporosis</subject><subject>Ovariectomy</subject><subject>Phosphorus</subject><subject>Proline</subject><subject>Raman spectroscopy</subject><subject>Rodents</subject><subject>Spectrum analysis</subject><subject>Teeth</subject><issn>0914-8779</issn><issn>1435-5604</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kd2K1TAUhYMoznH0AbyRgDfeRJM0bZJLGfwZGBD8Ae9Kmu6eydAmNUkP1JfyFU2nMyKCNwmb9a21SRZCzxl9zSiVb1I5pCCUKUI1rUjzAB2YqGpSN1Q8RAeqmSBKSn2GnqR0QymTtWSP0RnXlRCK8QP69eU6xEwyxAkb3-Mx-OM-wTCAzQmHAYeUIcwhhuTK7LHz1qUQcQbI17e2AaYQzYi74CFhOJlxMRl63K34s5mMx2kuYSXAhnm9dYCHeFxx74oSkzsB_k6i2TQzrtse53E4meiKL0zuZwmLJqen6NFgxgTP7u5z9O39u68XH8nVpw-XF2-viBUNz6QXwkKnus4qAMF7o5nuGsZVpbk2XEFNmyLKgWuoraDccMa0pEKaXlqg1Tl6tefOMfxYIOV2csnCOBoPYUkt00rXSklVFfTlP-hNWGJ5xkbpijYNp7xQbKds-YUUYWjn6CYT15bRdmuz3dtsS5vt1mbbFM-Lu-Slm6D_47ivrwB8B1KR_BHiX6v_m_obEmyuRg</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Paolillo, Fernanda Rossi</creator><creator>Romano, Renan Arnon</creator><creator>de Matos, Luciana</creator><creator>Martin, Airton Abrahão</creator><creator>Guimarães, Francisco Eduardo Gontijo</creator><creator>de Castro Neto, Jarbas Caiado</creator><creator>Bagnato, Vanderlei Salvador</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20190101</creationdate><title>Short-term and long-term effects of osteoporosis on incisor teeth and femoral bones evaluated by Raman spectroscopy and energy dispersive X-ray analysis in ovariectomized rats</title><author>Paolillo, Fernanda Rossi ; Romano, Renan Arnon ; de Matos, Luciana ; Martin, Airton Abrahão ; Guimarães, Francisco Eduardo Gontijo ; de Castro Neto, Jarbas Caiado ; Bagnato, Vanderlei Salvador</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-d44ceb8bbc8ee42da919b61283929a28e506bbc7f29e5c402a21197047ad7ce03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Age</topic><topic>Bone mass</topic><topic>Bones</topic><topic>Calcium</topic><topic>Femur</topic><topic>Long-term effects</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metabolic Diseases</topic><topic>Original Article</topic><topic>Orthopedics</topic><topic>Osteoporosis</topic><topic>Ovariectomy</topic><topic>Phosphorus</topic><topic>Proline</topic><topic>Raman spectroscopy</topic><topic>Rodents</topic><topic>Spectrum analysis</topic><topic>Teeth</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paolillo, Fernanda Rossi</creatorcontrib><creatorcontrib>Romano, Renan Arnon</creatorcontrib><creatorcontrib>de Matos, Luciana</creatorcontrib><creatorcontrib>Martin, Airton Abrahão</creatorcontrib><creatorcontrib>Guimarães, Francisco Eduardo Gontijo</creatorcontrib><creatorcontrib>de Castro Neto, Jarbas Caiado</creatorcontrib><creatorcontrib>Bagnato, Vanderlei Salvador</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bone and mineral metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paolillo, Fernanda Rossi</au><au>Romano, Renan Arnon</au><au>de Matos, Luciana</au><au>Martin, Airton Abrahão</au><au>Guimarães, Francisco Eduardo Gontijo</au><au>de Castro Neto, Jarbas Caiado</au><au>Bagnato, Vanderlei Salvador</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Short-term and long-term effects of osteoporosis on incisor teeth and femoral bones evaluated by Raman spectroscopy and energy dispersive X-ray analysis in ovariectomized rats</atitle><jtitle>Journal of bone and mineral metabolism</jtitle><stitle>J Bone Miner Metab</stitle><addtitle>J Bone Miner Metab</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>37</volume><issue>1</issue><spage>18</spage><epage>27</epage><pages>18-27</pages><issn>0914-8779</issn><eissn>1435-5604</eissn><abstract>There are few published data on the relationship between loss of bone mass due to osteoporosis and poor tooth quality. This study analyzed the effects of osteoporosis on incisor teeth and femoral bones using optical techniques in rats. Twenty female Wistar rats aged 6 months (
n
= 20) were randomized into two groups: control group, non-ovariectomized rats (
n
= 10); ovariectomy group, ovariectomized rats to induce osteoporosis (
n
= 10). Each group was subdivided randomly into two groups containing five rats each as follows. Control group 1: non-ovariectomized rats euthanized at the age of 9 or 3 months post-ovariectomy (
n
= 5); Control group 2: non-ovariectomized rats euthanized at the age of 1 year or 6 months post-ovariectomy (
n
= 5); ovariectomy group 1: ovariectomized rats euthanized at the age of 9 months or 3 months post-ovariectomy (
n
= 5); ovariectomy group 2: ovariectomized rats euthanized at the age of 1 year or 6 months post-ovariectomy (
n
= 5). The incisor teeth and femoral bones of Wistar rats were removed to perform Raman spectroscopy using an excitation laser at 785 nm. In addition, an energy-dispersive X-ray spectrometer system was used to evaluate calcium (Ca) and phosphorus (P). The main findings included significant changes (
p
< 0.05) for phosphate and carbonate band areas for both incisor teeth and femur bones. In addition, there was significant negative correlation between the P concentration and phosphate/carbonate ratio (lower P content–larger ratio,
p
< 0.05) for incisor teeth and femoral bones. The proline and CH
2
wag band areas were significantly reduced only for the incisor teeth (
p
< 0.05). Therefore, Raman spectroscopy assessed the compositional, physicochemical and structural changes in hard tissue. The current study also pointed out the possible action mechanisms of these changes, bone fracture risk and dental fragility. It is important to emphasize that poor dental quality may also occur due to osteoporosis.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><pmid>29344812</pmid><doi>10.1007/s00774-018-0903-6</doi><tpages>10</tpages></addata></record> |
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| subjects | Age Bone mass Bones Calcium Femur Long-term effects Medicine Medicine & Public Health Metabolic Diseases Original Article Orthopedics Osteoporosis Ovariectomy Phosphorus Proline Raman spectroscopy Rodents Spectrum analysis Teeth |
| title | Short-term and long-term effects of osteoporosis on incisor teeth and femoral bones evaluated by Raman spectroscopy and energy dispersive X-ray analysis in ovariectomized rats |
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