Alterations of oxygen consumption and extracellular acidification rates by glutamine in PBMCs of SLE patients
We evaluated plasma glutamine levels and basal mitochondrial oxygen consumption rate (mOCR ) and basal extracellular acidification rate (ECAR ) of peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematous (SLE) patients and healthy controls (HCs). Lower plasma glutamine levels correl...
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| Veröffentlicht in: | Mitochondrion 2019-01, Vol.44, p.65-74 |
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| container_title | Mitochondrion |
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| creator | Lee, Hui-Ting Lin, Chen-Sung Pan, Siao-Cian Wu, Tsai-Hung Lee, Chyou-Shen Chang, Deh-Ming Tsai, Chang-Youh Wei, Yau-Huei |
| description | We evaluated plasma glutamine levels and basal mitochondrial oxygen consumption rate (mOCR
) and basal extracellular acidification rate (ECAR
) of peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematous (SLE) patients and healthy controls (HCs). Lower plasma glutamine levels correlated with higher SLE disease activity indexes (p=0.025). Incubated in DMEM containing 100mg/dL glucose, SLE-PBMCs displayed lower mOCR
(p=0.018) but similar ECAR
(p=0.467) to those of HC-PBMCs, and their mOCR
got elevated (p |
| doi_str_mv | 10.1016/j.mito.2018.01.002 |
| format | Article |
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) and basal extracellular acidification rate (ECAR
) of peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematous (SLE) patients and healthy controls (HCs). Lower plasma glutamine levels correlated with higher SLE disease activity indexes (p=0.025). Incubated in DMEM containing 100mg/dL glucose, SLE-PBMCs displayed lower mOCR
(p=0.018) but similar ECAR
(p=0.467) to those of HC-PBMCs, and their mOCR
got elevated (p<0.001) without altering ECAR
(p=0.239) by supplementation with 2 or 4mM glutamine. We conclude that impaired mitochondrial respiration of SLE-PBMCs could be improved by glutamine under euglycemic condition.</description><identifier>ISSN: 1567-7249</identifier><identifier>EISSN: 1872-8278</identifier><identifier>DOI: 10.1016/j.mito.2018.01.002</identifier><identifier>PMID: 29337141</identifier><language>eng</language><publisher>Netherlands</publisher><ispartof>Mitochondrion, 2019-01, Vol.44, p.65-74</ispartof><rights>Copyright © 2018. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c303t-41467905474fc23bf04d618ceded3a4acbf1648720fbc221eb03444521dea993</citedby><cites>FETCH-LOGICAL-c303t-41467905474fc23bf04d618ceded3a4acbf1648720fbc221eb03444521dea993</cites><orcidid>0000-0002-4154-4018</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29337141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Hui-Ting</creatorcontrib><creatorcontrib>Lin, Chen-Sung</creatorcontrib><creatorcontrib>Pan, Siao-Cian</creatorcontrib><creatorcontrib>Wu, Tsai-Hung</creatorcontrib><creatorcontrib>Lee, Chyou-Shen</creatorcontrib><creatorcontrib>Chang, Deh-Ming</creatorcontrib><creatorcontrib>Tsai, Chang-Youh</creatorcontrib><creatorcontrib>Wei, Yau-Huei</creatorcontrib><title>Alterations of oxygen consumption and extracellular acidification rates by glutamine in PBMCs of SLE patients</title><title>Mitochondrion</title><addtitle>Mitochondrion</addtitle><description>We evaluated plasma glutamine levels and basal mitochondrial oxygen consumption rate (mOCR
) and basal extracellular acidification rate (ECAR
) of peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematous (SLE) patients and healthy controls (HCs). Lower plasma glutamine levels correlated with higher SLE disease activity indexes (p=0.025). Incubated in DMEM containing 100mg/dL glucose, SLE-PBMCs displayed lower mOCR
(p=0.018) but similar ECAR
(p=0.467) to those of HC-PBMCs, and their mOCR
got elevated (p<0.001) without altering ECAR
(p=0.239) by supplementation with 2 or 4mM glutamine. We conclude that impaired mitochondrial respiration of SLE-PBMCs could be improved by glutamine under euglycemic condition.</description><issn>1567-7249</issn><issn>1872-8278</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNo9kMtOwzAQRS0EoqXwAyyQl2wSPLYTx8tSlYdUBBLdW47jVK7yKHYitX9P0hZW89C9VzMHoXsgMRBIn7Zx7bo2pgSymEBMCL1AU8gEjTIqssuhT1IRCcrlBN2EsCUEBFB6jSZUMiaAwxTV86qzXneubQJuS9zuDxvbYDOMfb0b11g3Bbb7zmtjq6qvtMfauMKVzhxteHDbgPMD3lR9p2vXWOwa_PX8sTgmfq-WeDcobdOFW3RV6irYu3OdofXLcr14i1afr--L-SoyjLAu4sBTIUnCBS8NZXlJeJFCZmxhC6a5NnkJKR8eJWVuKAWbE8Y5TygUVkvJZujxFLvz7U9vQ6dqF8brdWPbPiiQmUwyYJIPUnqSGt-G4G2pdt7V2h8UEDVSVls1UlYjZUVADZQH08M5v89rW_xb_rCyX5WGetc</recordid><startdate>201901</startdate><enddate>201901</enddate><creator>Lee, Hui-Ting</creator><creator>Lin, Chen-Sung</creator><creator>Pan, Siao-Cian</creator><creator>Wu, Tsai-Hung</creator><creator>Lee, Chyou-Shen</creator><creator>Chang, Deh-Ming</creator><creator>Tsai, Chang-Youh</creator><creator>Wei, Yau-Huei</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4154-4018</orcidid></search><sort><creationdate>201901</creationdate><title>Alterations of oxygen consumption and extracellular acidification rates by glutamine in PBMCs of SLE patients</title><author>Lee, Hui-Ting ; Lin, Chen-Sung ; Pan, Siao-Cian ; Wu, Tsai-Hung ; Lee, Chyou-Shen ; Chang, Deh-Ming ; Tsai, Chang-Youh ; Wei, Yau-Huei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-41467905474fc23bf04d618ceded3a4acbf1648720fbc221eb03444521dea993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Hui-Ting</creatorcontrib><creatorcontrib>Lin, Chen-Sung</creatorcontrib><creatorcontrib>Pan, Siao-Cian</creatorcontrib><creatorcontrib>Wu, Tsai-Hung</creatorcontrib><creatorcontrib>Lee, Chyou-Shen</creatorcontrib><creatorcontrib>Chang, Deh-Ming</creatorcontrib><creatorcontrib>Tsai, Chang-Youh</creatorcontrib><creatorcontrib>Wei, Yau-Huei</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mitochondrion</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Hui-Ting</au><au>Lin, Chen-Sung</au><au>Pan, Siao-Cian</au><au>Wu, Tsai-Hung</au><au>Lee, Chyou-Shen</au><au>Chang, Deh-Ming</au><au>Tsai, Chang-Youh</au><au>Wei, Yau-Huei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alterations of oxygen consumption and extracellular acidification rates by glutamine in PBMCs of SLE patients</atitle><jtitle>Mitochondrion</jtitle><addtitle>Mitochondrion</addtitle><date>2019-01</date><risdate>2019</risdate><volume>44</volume><spage>65</spage><epage>74</epage><pages>65-74</pages><issn>1567-7249</issn><eissn>1872-8278</eissn><abstract>We evaluated plasma glutamine levels and basal mitochondrial oxygen consumption rate (mOCR
) and basal extracellular acidification rate (ECAR
) of peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematous (SLE) patients and healthy controls (HCs). Lower plasma glutamine levels correlated with higher SLE disease activity indexes (p=0.025). Incubated in DMEM containing 100mg/dL glucose, SLE-PBMCs displayed lower mOCR
(p=0.018) but similar ECAR
(p=0.467) to those of HC-PBMCs, and their mOCR
got elevated (p<0.001) without altering ECAR
(p=0.239) by supplementation with 2 or 4mM glutamine. We conclude that impaired mitochondrial respiration of SLE-PBMCs could be improved by glutamine under euglycemic condition.</abstract><cop>Netherlands</cop><pmid>29337141</pmid><doi>10.1016/j.mito.2018.01.002</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-4154-4018</orcidid></addata></record> |
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| title | Alterations of oxygen consumption and extracellular acidification rates by glutamine in PBMCs of SLE patients |
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