Bioinformatic analyses of zona pellucida genes in vertebrates and their expression in Nile tilapia
Zona pellucida ( ZP ) genes encode ZP glycoproteins which constitute the coat surrounding oocytes and early embryos. Genome-wide identification of ZP genes is still lacking in vertebrates, especially in fish species. Herein, we conducted bioinformatic analyses of the ZP genes of the Nile tilapia and...
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| Veröffentlicht in: | Fish physiology and biochemistry 2018-04, Vol.44 (2), p.435-449 |
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| creator | Wu, Tianli Cheng, Yunying Liu, Zhilong Tao, Wenjing Zheng, Shuqing Wang, Deshou |
| description | Zona pellucida
(
ZP
) genes encode ZP glycoproteins which constitute the coat surrounding oocytes and early embryos. Genome-wide identification of ZP genes is still lacking in vertebrates, especially in fish species. Herein, we conducted bioinformatic analyses of the ZP genes of the Nile tilapia and other vertebrates. Totally 16, 9, 17, 27, 21, 20, 26, 19, 14,11, 24, 17, 9, 18, 8, 11, 9, 8, 5, and 4
ZP
genes belonging to 5 subfamilies (
ZPA
,
ZPB
,
ZPC
,
ZPD
, and
ZPAX
) were found in the sea lamprey, elephant shark, coelacanth, spotted gar, zebrafish, medaka, stickleback, Nile tilapia, Amazon molly, platyfish, seahorse, Northern snakehead, cavefish, tetraodon, clawed frog, turtle, chicken, platypus, kangaroo rat, and human genomes, respectively. The expansion of
ZP
genes in basal vertebrates was mainly achieved by gene duplication of
ZPB
,
ZPC
, and
ZPAX
subfamilies, while the shrink of
ZP
gene number in viviparous mammals was achieved by keeping only one copy of the ZP genes in each subfamily or even secondary loss of some subfamilies. The number of
ZP
gene is related to the environment where the eggs are fertilized and the embryos develop in vertebrates. Transcriptomic analysis showed that 14
ZP
genes were expressed in the ovary of Nile tilapia, while two (
ZPB2b
and
ZPC2
) were highly expressed in the liver. On the other hand,
ZPB1a
and
ZPB2c
were not found to be expressed in any tissue or at any developmental stage of the gonads examined. In the ovary, the expression of
ZP
genes started from 30 dah (days after hatching), significantly upregulated at 90 dah and maintained this level at 180 dah. The expression of
ZPC2
in the liver and
ZPC5-2
and
ZPAX1
in the ovary was confirmed by in situ hybridization. The ovary- and liver-expressed
ZP
genes are expressed coordinately with oocyte growth in tilapia. |
| doi_str_mv | 10.1007/s10695-017-0434-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1989559147</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1989559147</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-a1034b05f111ead717e94fd755bdb7093f7a61949ca0ec6fab015ece6e3c90033</originalsourceid><addsrcrecordid>eNp1kUFv1TAMxyMEYm8bH4ALisRll4LdJM1y3CYGSBO7bOcoTd2RqS8pSTsxPj15vIEQEifL9s__xP4z9hrhHQLo9wWhM6oB1A1IIRv5jG1QadEo7E6fsw2YFhrUsj1gh6XcA4DRHb5kB60RoBHVhvXnIYU4prx1S_DcRTc9Fio8jfxHio7PNE2rD4PjdxRrPUT-QHmhPrulpi4OfPlKIXP6PmcqJaS4Y76EifgSJjcHd8xejG4q9OopHrHbyw83F5-aq-uPny_OrhovdLs0DkHIHtSIiOQGjZqMHAetVD_0GowYtevQSOMdkO9G1wMq8tSR8AZAiCN2stedc_q2UlnsNhRf_-8ipbVYNKdGKYNSV_TtP-h9WnPd_RelWtnptq0U7imfUymZRjvnsHX50SLYnQF2b4CtBtidAVbWmTdPymu_peHPxO-LV6DdA6W24h3lv57-r-pPOtKQ-A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1985246722</pqid></control><display><type>article</type><title>Bioinformatic analyses of zona pellucida genes in vertebrates and their expression in Nile tilapia</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Wu, Tianli ; Cheng, Yunying ; Liu, Zhilong ; Tao, Wenjing ; Zheng, Shuqing ; Wang, Deshou</creator><creatorcontrib>Wu, Tianli ; Cheng, Yunying ; Liu, Zhilong ; Tao, Wenjing ; Zheng, Shuqing ; Wang, Deshou</creatorcontrib><description>Zona pellucida
(
ZP
) genes encode ZP glycoproteins which constitute the coat surrounding oocytes and early embryos. Genome-wide identification of ZP genes is still lacking in vertebrates, especially in fish species. Herein, we conducted bioinformatic analyses of the ZP genes of the Nile tilapia and other vertebrates. Totally 16, 9, 17, 27, 21, 20, 26, 19, 14,11, 24, 17, 9, 18, 8, 11, 9, 8, 5, and 4
ZP
genes belonging to 5 subfamilies (
ZPA
,
ZPB
,
ZPC
,
ZPD
, and
ZPAX
) were found in the sea lamprey, elephant shark, coelacanth, spotted gar, zebrafish, medaka, stickleback, Nile tilapia, Amazon molly, platyfish, seahorse, Northern snakehead, cavefish, tetraodon, clawed frog, turtle, chicken, platypus, kangaroo rat, and human genomes, respectively. The expansion of
ZP
genes in basal vertebrates was mainly achieved by gene duplication of
ZPB
,
ZPC
, and
ZPAX
subfamilies, while the shrink of
ZP
gene number in viviparous mammals was achieved by keeping only one copy of the ZP genes in each subfamily or even secondary loss of some subfamilies. The number of
ZP
gene is related to the environment where the eggs are fertilized and the embryos develop in vertebrates. Transcriptomic analysis showed that 14
ZP
genes were expressed in the ovary of Nile tilapia, while two (
ZPB2b
and
ZPC2
) were highly expressed in the liver. On the other hand,
ZPB1a
and
ZPB2c
were not found to be expressed in any tissue or at any developmental stage of the gonads examined. In the ovary, the expression of
ZP
genes started from 30 dah (days after hatching), significantly upregulated at 90 dah and maintained this level at 180 dah. The expression of
ZPC2
in the liver and
ZPC5-2
and
ZPAX1
in the ovary was confirmed by in situ hybridization. The ovary- and liver-expressed
ZP
genes are expressed coordinately with oocyte growth in tilapia.</description><identifier>ISSN: 0920-1742</identifier><identifier>EISSN: 1573-5168</identifier><identifier>DOI: 10.1007/s10695-017-0434-4</identifier><identifier>PMID: 29307115</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Animal embryos ; Animal Physiology ; Animals ; Aquatic mammals ; Biomedical and Life Sciences ; Cichlids - genetics ; Cichlids - physiology ; Computational Biology - methods ; Developmental stages ; Egg Proteins - genetics ; Egg Proteins - metabolism ; Eggs ; Embryos ; Fish ; Fish eggs ; Fish Proteins - metabolism ; Freshwater & Marine Ecology ; Freshwater fishes ; Freshwater mammals ; Gene duplication ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Genes ; Genome ; Genomes ; Glycoproteins ; Gonads ; Gonads - metabolism ; Hatching ; Histology ; Life Sciences ; Liver ; Marine fishes ; Morphology ; Oocytes ; Oreochromis niloticus ; Ovaries ; Phylogeny ; Tilapia ; Tissue ; Vertebrates ; Vertebrates - genetics ; Vertebrates - metabolism ; Viviparity ; Zebrafish ; Zona pellucida ; Zona Pellucida - metabolism ; Zona Pellucida Glycoproteins - genetics ; Zona Pellucida Glycoproteins - metabolism ; Zoology ; Zp gene</subject><ispartof>Fish physiology and biochemistry, 2018-04, Vol.44 (2), p.435-449</ispartof><rights>Springer Science+Business Media B.V. 2018</rights><rights>Fish Physiology and Biochemistry is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-a1034b05f111ead717e94fd755bdb7093f7a61949ca0ec6fab015ece6e3c90033</citedby><cites>FETCH-LOGICAL-c372t-a1034b05f111ead717e94fd755bdb7093f7a61949ca0ec6fab015ece6e3c90033</cites><orcidid>0000-0002-4967-524X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10695-017-0434-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10695-017-0434-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29307115$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Tianli</creatorcontrib><creatorcontrib>Cheng, Yunying</creatorcontrib><creatorcontrib>Liu, Zhilong</creatorcontrib><creatorcontrib>Tao, Wenjing</creatorcontrib><creatorcontrib>Zheng, Shuqing</creatorcontrib><creatorcontrib>Wang, Deshou</creatorcontrib><title>Bioinformatic analyses of zona pellucida genes in vertebrates and their expression in Nile tilapia</title><title>Fish physiology and biochemistry</title><addtitle>Fish Physiol Biochem</addtitle><addtitle>Fish Physiol Biochem</addtitle><description>Zona pellucida
(
ZP
) genes encode ZP glycoproteins which constitute the coat surrounding oocytes and early embryos. Genome-wide identification of ZP genes is still lacking in vertebrates, especially in fish species. Herein, we conducted bioinformatic analyses of the ZP genes of the Nile tilapia and other vertebrates. Totally 16, 9, 17, 27, 21, 20, 26, 19, 14,11, 24, 17, 9, 18, 8, 11, 9, 8, 5, and 4
ZP
genes belonging to 5 subfamilies (
ZPA
,
ZPB
,
ZPC
,
ZPD
, and
ZPAX
) were found in the sea lamprey, elephant shark, coelacanth, spotted gar, zebrafish, medaka, stickleback, Nile tilapia, Amazon molly, platyfish, seahorse, Northern snakehead, cavefish, tetraodon, clawed frog, turtle, chicken, platypus, kangaroo rat, and human genomes, respectively. The expansion of
ZP
genes in basal vertebrates was mainly achieved by gene duplication of
ZPB
,
ZPC
, and
ZPAX
subfamilies, while the shrink of
ZP
gene number in viviparous mammals was achieved by keeping only one copy of the ZP genes in each subfamily or even secondary loss of some subfamilies. The number of
ZP
gene is related to the environment where the eggs are fertilized and the embryos develop in vertebrates. Transcriptomic analysis showed that 14
ZP
genes were expressed in the ovary of Nile tilapia, while two (
ZPB2b
and
ZPC2
) were highly expressed in the liver. On the other hand,
ZPB1a
and
ZPB2c
were not found to be expressed in any tissue or at any developmental stage of the gonads examined. In the ovary, the expression of
ZP
genes started from 30 dah (days after hatching), significantly upregulated at 90 dah and maintained this level at 180 dah. The expression of
ZPC2
in the liver and
ZPC5-2
and
ZPAX1
in the ovary was confirmed by in situ hybridization. The ovary- and liver-expressed
ZP
genes are expressed coordinately with oocyte growth in tilapia.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animal embryos</subject><subject>Animal Physiology</subject><subject>Animals</subject><subject>Aquatic mammals</subject><subject>Biomedical and Life Sciences</subject><subject>Cichlids - genetics</subject><subject>Cichlids - physiology</subject><subject>Computational Biology - methods</subject><subject>Developmental stages</subject><subject>Egg Proteins - genetics</subject><subject>Egg Proteins - metabolism</subject><subject>Eggs</subject><subject>Embryos</subject><subject>Fish</subject><subject>Fish eggs</subject><subject>Fish Proteins - metabolism</subject><subject>Freshwater & Marine Ecology</subject><subject>Freshwater fishes</subject><subject>Freshwater mammals</subject><subject>Gene duplication</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes</subject><subject>Genome</subject><subject>Genomes</subject><subject>Glycoproteins</subject><subject>Gonads</subject><subject>Gonads - metabolism</subject><subject>Hatching</subject><subject>Histology</subject><subject>Life Sciences</subject><subject>Liver</subject><subject>Marine fishes</subject><subject>Morphology</subject><subject>Oocytes</subject><subject>Oreochromis niloticus</subject><subject>Ovaries</subject><subject>Phylogeny</subject><subject>Tilapia</subject><subject>Tissue</subject><subject>Vertebrates</subject><subject>Vertebrates - genetics</subject><subject>Vertebrates - metabolism</subject><subject>Viviparity</subject><subject>Zebrafish</subject><subject>Zona pellucida</subject><subject>Zona Pellucida - metabolism</subject><subject>Zona Pellucida Glycoproteins - genetics</subject><subject>Zona Pellucida Glycoproteins - metabolism</subject><subject>Zoology</subject><subject>Zp gene</subject><issn>0920-1742</issn><issn>1573-5168</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kUFv1TAMxyMEYm8bH4ALisRll4LdJM1y3CYGSBO7bOcoTd2RqS8pSTsxPj15vIEQEifL9s__xP4z9hrhHQLo9wWhM6oB1A1IIRv5jG1QadEo7E6fsw2YFhrUsj1gh6XcA4DRHb5kB60RoBHVhvXnIYU4prx1S_DcRTc9Fio8jfxHio7PNE2rD4PjdxRrPUT-QHmhPrulpi4OfPlKIXP6PmcqJaS4Y76EifgSJjcHd8xejG4q9OopHrHbyw83F5-aq-uPny_OrhovdLs0DkHIHtSIiOQGjZqMHAetVD_0GowYtevQSOMdkO9G1wMq8tSR8AZAiCN2stedc_q2UlnsNhRf_-8ipbVYNKdGKYNSV_TtP-h9WnPd_RelWtnptq0U7imfUymZRjvnsHX50SLYnQF2b4CtBtidAVbWmTdPymu_peHPxO-LV6DdA6W24h3lv57-r-pPOtKQ-A</recordid><startdate>20180401</startdate><enddate>20180401</enddate><creator>Wu, Tianli</creator><creator>Cheng, Yunying</creator><creator>Liu, Zhilong</creator><creator>Tao, Wenjing</creator><creator>Zheng, Shuqing</creator><creator>Wang, Deshou</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.F</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4967-524X</orcidid></search><sort><creationdate>20180401</creationdate><title>Bioinformatic analyses of zona pellucida genes in vertebrates and their expression in Nile tilapia</title><author>Wu, Tianli ; Cheng, Yunying ; Liu, Zhilong ; Tao, Wenjing ; Zheng, Shuqing ; Wang, Deshou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-a1034b05f111ead717e94fd755bdb7093f7a61949ca0ec6fab015ece6e3c90033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Animal embryos</topic><topic>Animal Physiology</topic><topic>Animals</topic><topic>Aquatic mammals</topic><topic>Biomedical and Life Sciences</topic><topic>Cichlids - genetics</topic><topic>Cichlids - physiology</topic><topic>Computational Biology - methods</topic><topic>Developmental stages</topic><topic>Egg Proteins - genetics</topic><topic>Egg Proteins - metabolism</topic><topic>Eggs</topic><topic>Embryos</topic><topic>Fish</topic><topic>Fish eggs</topic><topic>Fish Proteins - metabolism</topic><topic>Freshwater & Marine Ecology</topic><topic>Freshwater fishes</topic><topic>Freshwater mammals</topic><topic>Gene duplication</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genes</topic><topic>Genome</topic><topic>Genomes</topic><topic>Glycoproteins</topic><topic>Gonads</topic><topic>Gonads - metabolism</topic><topic>Hatching</topic><topic>Histology</topic><topic>Life Sciences</topic><topic>Liver</topic><topic>Marine fishes</topic><topic>Morphology</topic><topic>Oocytes</topic><topic>Oreochromis niloticus</topic><topic>Ovaries</topic><topic>Phylogeny</topic><topic>Tilapia</topic><topic>Tissue</topic><topic>Vertebrates</topic><topic>Vertebrates - genetics</topic><topic>Vertebrates - metabolism</topic><topic>Viviparity</topic><topic>Zebrafish</topic><topic>Zona pellucida</topic><topic>Zona Pellucida - metabolism</topic><topic>Zona Pellucida Glycoproteins - genetics</topic><topic>Zona Pellucida Glycoproteins - metabolism</topic><topic>Zoology</topic><topic>Zp gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Tianli</creatorcontrib><creatorcontrib>Cheng, Yunying</creatorcontrib><creatorcontrib>Liu, Zhilong</creatorcontrib><creatorcontrib>Tao, Wenjing</creatorcontrib><creatorcontrib>Zheng, Shuqing</creatorcontrib><creatorcontrib>Wang, Deshou</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Fish physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Tianli</au><au>Cheng, Yunying</au><au>Liu, Zhilong</au><au>Tao, Wenjing</au><au>Zheng, Shuqing</au><au>Wang, Deshou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioinformatic analyses of zona pellucida genes in vertebrates and their expression in Nile tilapia</atitle><jtitle>Fish physiology and biochemistry</jtitle><stitle>Fish Physiol Biochem</stitle><addtitle>Fish Physiol Biochem</addtitle><date>2018-04-01</date><risdate>2018</risdate><volume>44</volume><issue>2</issue><spage>435</spage><epage>449</epage><pages>435-449</pages><issn>0920-1742</issn><eissn>1573-5168</eissn><abstract>Zona pellucida
(
ZP
) genes encode ZP glycoproteins which constitute the coat surrounding oocytes and early embryos. Genome-wide identification of ZP genes is still lacking in vertebrates, especially in fish species. Herein, we conducted bioinformatic analyses of the ZP genes of the Nile tilapia and other vertebrates. Totally 16, 9, 17, 27, 21, 20, 26, 19, 14,11, 24, 17, 9, 18, 8, 11, 9, 8, 5, and 4
ZP
genes belonging to 5 subfamilies (
ZPA
,
ZPB
,
ZPC
,
ZPD
, and
ZPAX
) were found in the sea lamprey, elephant shark, coelacanth, spotted gar, zebrafish, medaka, stickleback, Nile tilapia, Amazon molly, platyfish, seahorse, Northern snakehead, cavefish, tetraodon, clawed frog, turtle, chicken, platypus, kangaroo rat, and human genomes, respectively. The expansion of
ZP
genes in basal vertebrates was mainly achieved by gene duplication of
ZPB
,
ZPC
, and
ZPAX
subfamilies, while the shrink of
ZP
gene number in viviparous mammals was achieved by keeping only one copy of the ZP genes in each subfamily or even secondary loss of some subfamilies. The number of
ZP
gene is related to the environment where the eggs are fertilized and the embryos develop in vertebrates. Transcriptomic analysis showed that 14
ZP
genes were expressed in the ovary of Nile tilapia, while two (
ZPB2b
and
ZPC2
) were highly expressed in the liver. On the other hand,
ZPB1a
and
ZPB2c
were not found to be expressed in any tissue or at any developmental stage of the gonads examined. In the ovary, the expression of
ZP
genes started from 30 dah (days after hatching), significantly upregulated at 90 dah and maintained this level at 180 dah. The expression of
ZPC2
in the liver and
ZPC5-2
and
ZPAX1
in the ovary was confirmed by in situ hybridization. The ovary- and liver-expressed
ZP
genes are expressed coordinately with oocyte growth in tilapia.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>29307115</pmid><doi>10.1007/s10695-017-0434-4</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-4967-524X</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0920-1742 |
| ispartof | Fish physiology and biochemistry, 2018-04, Vol.44 (2), p.435-449 |
| issn | 0920-1742 1573-5168 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1989559147 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Animal Anatomy Animal Biochemistry Animal embryos Animal Physiology Animals Aquatic mammals Biomedical and Life Sciences Cichlids - genetics Cichlids - physiology Computational Biology - methods Developmental stages Egg Proteins - genetics Egg Proteins - metabolism Eggs Embryos Fish Fish eggs Fish Proteins - metabolism Freshwater & Marine Ecology Freshwater fishes Freshwater mammals Gene duplication Gene expression Gene Expression Profiling Gene Expression Regulation, Developmental Genes Genome Genomes Glycoproteins Gonads Gonads - metabolism Hatching Histology Life Sciences Liver Marine fishes Morphology Oocytes Oreochromis niloticus Ovaries Phylogeny Tilapia Tissue Vertebrates Vertebrates - genetics Vertebrates - metabolism Viviparity Zebrafish Zona pellucida Zona Pellucida - metabolism Zona Pellucida Glycoproteins - genetics Zona Pellucida Glycoproteins - metabolism Zoology Zp gene |
| title | Bioinformatic analyses of zona pellucida genes in vertebrates and their expression in Nile tilapia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T13%3A44%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bioinformatic%20analyses%20of%20zona%20pellucida%20genes%20in%20vertebrates%20and%20their%20expression%20in%20Nile%20tilapia&rft.jtitle=Fish%20physiology%20and%20biochemistry&rft.au=Wu,%20Tianli&rft.date=2018-04-01&rft.volume=44&rft.issue=2&rft.spage=435&rft.epage=449&rft.pages=435-449&rft.issn=0920-1742&rft.eissn=1573-5168&rft_id=info:doi/10.1007/s10695-017-0434-4&rft_dat=%3Cproquest_cross%3E1989559147%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1985246722&rft_id=info:pmid/29307115&rfr_iscdi=true |