Rational synthesis of MoS2-based immobilized trypsin for rapid and effective protein digestion

In this work, a novel MoS2-based immobilized trypsin reactor was designed and prepared. Pyrene-1-butyric acid was first assembled onto MoS2 nanosheets via the strong π-π stacking and then trypsin was covalently immobilized onto the nanocomposite supports through amidation reaction. Compared with tra...

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Veröffentlicht in:	Talanta (Oxford) 2018-03, Vol.179, p.393-400
Hauptverfasser:	Xia, Chaoshuang, Wang, Heping, Jiao, Fenglong, Gao, Fangyuan, Wu, Qiong, Shen, Yehua, Zhang, Yangjun, Qian, Xiaohong
Format:	Artikel
Sprache:	eng
Schlagworte:	Abiotic stress Amygdalus Pedunculata Pall Immobilized trypsin reactor Molybdenum disulfide Proteomics Two-dimensional nanomaterial
Online-Zugang:	Volltext
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