Liposome-Assisted Metabolic Glycan Labeling With Cell and Tissue Selectivity
Metabolic labeling of glycans with sugar chemical reporters (i.e., unnatural sugars bearing a bioorthogonal group), followed by bioorthogonal reaction with imaging probes or affinity tags, has enabled visualization and proteomic analysis of glycosylation in live cells and in living animals. This two...
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| Veröffentlicht in: | Methods in enzymology 2018, Vol.598, p.321-353 |
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| container_title | Methods in enzymology |
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| creator | Du, Yifei Xie, Ran Sun, Yuting Fan, Xinqi Chen, Xing |
| description | Metabolic labeling of glycans with sugar chemical reporters (i.e., unnatural sugars bearing a bioorthogonal group), followed by bioorthogonal reaction with imaging probes or affinity tags, has enabled visualization and proteomic analysis of glycosylation in live cells and in living animals. This two-step metabolic glycan labeling strategy has emerged as a powerful tool for probing glycosylation, but suffers from a lack of cell-type selectivity. Here we describe liposome-assisted bioorthogonal reporter (LABOR), a liposome-assisted format of metabolic glycan labeling that allows for cell-selective and tissue-specific glycan imaging and glycoproteomic profiling. After a brief introduction of the principles and applications of LABOR, we provide detailed protocols for performing LABOR in cell culture and in living mice. |
| doi_str_mv | 10.1016/bs.mie.2017.06.037 |
| format | Article |
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This two-step metabolic glycan labeling strategy has emerged as a powerful tool for probing glycosylation, but suffers from a lack of cell-type selectivity. Here we describe liposome-assisted bioorthogonal reporter (LABOR), a liposome-assisted format of metabolic glycan labeling that allows for cell-selective and tissue-specific glycan imaging and glycoproteomic profiling. 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After a brief introduction of the principles and applications of LABOR, we provide detailed protocols for performing LABOR in cell culture and in living mice.</description><subject>Animals</subject><subject>Cell Culture Techniques - instrumentation</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Line, Tumor</subject><subject>Coculture Techniques - instrumentation</subject><subject>Coculture Techniques - methods</subject><subject>Dynamic Light Scattering - instrumentation</subject><subject>Dynamic Light Scattering - methods</subject><subject>Flow Cytometry - instrumentation</subject><subject>Flow Cytometry - methods</subject><subject>Glycosylation</subject><subject>Humans</subject><subject>Indicators and Reagents - chemistry</subject><subject>Liposomes - chemistry</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Molecular Imaging - instrumentation</subject><subject>Molecular Imaging - methods</subject><subject>N-Acetylneuraminic Acid - chemistry</subject><subject>Optical Imaging - instrumentation</subject><subject>Optical Imaging - methods</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - metabolism</subject><subject>Proteomics - instrumentation</subject><subject>Proteomics - methods</subject><subject>Staining and Labeling - instrumentation</subject><subject>Staining and Labeling - methods</subject><subject>Xenograft Model Antitumor Assays - instrumentation</subject><subject>Xenograft Model Antitumor Assays - methods</subject><issn>1557-7988</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kM1KAzEYRYMgtlZfwIVk6WbGL8nkb1mKVmHEhRWXQ5L5qpH5qU1G6NurqKuzORwul5ALBiUDpq59KvuIJQemS1AlCH1E5kxKXWhrzIycpvQOwLWx7ITMuBWgqorNSV3H3ZjGHotlSjFlbOkDZufHLga67g7BDbR2Hrs4vNKXmN_oCruOuqGlm5jShPQJOww5fsZ8OCPHW9clPP_jgjzf3mxWd0X9uL5fLesicAW5CFqFyouAUvo2yAqB8SAZaMtB6WqrXEBuwHkDW62Ubg1HJ0FZppwKKMSCXP12d_vxY8KUmz6m8L3LDThOqWHWWCmsNj_q5Z86-R7bZrePvdsfmv8HxBcTqlwi</recordid><startdate>2018</startdate><enddate>2018</enddate><creator>Du, Yifei</creator><creator>Xie, Ran</creator><creator>Sun, Yuting</creator><creator>Fan, Xinqi</creator><creator>Chen, Xing</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2018</creationdate><title>Liposome-Assisted Metabolic Glycan Labeling With Cell and Tissue Selectivity</title><author>Du, Yifei ; 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This two-step metabolic glycan labeling strategy has emerged as a powerful tool for probing glycosylation, but suffers from a lack of cell-type selectivity. Here we describe liposome-assisted bioorthogonal reporter (LABOR), a liposome-assisted format of metabolic glycan labeling that allows for cell-selective and tissue-specific glycan imaging and glycoproteomic profiling. After a brief introduction of the principles and applications of LABOR, we provide detailed protocols for performing LABOR in cell culture and in living mice.</abstract><cop>United States</cop><pmid>29306441</pmid><doi>10.1016/bs.mie.2017.06.037</doi><tpages>33</tpages></addata></record> |
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| subjects | Animals Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cell Line, Tumor Coculture Techniques - instrumentation Coculture Techniques - methods Dynamic Light Scattering - instrumentation Dynamic Light Scattering - methods Flow Cytometry - instrumentation Flow Cytometry - methods Glycosylation Humans Indicators and Reagents - chemistry Liposomes - chemistry Mice Mice, Inbred BALB C Mice, Nude Molecular Imaging - instrumentation Molecular Imaging - methods N-Acetylneuraminic Acid - chemistry Optical Imaging - instrumentation Optical Imaging - methods Polysaccharides - chemistry Polysaccharides - metabolism Proteomics - instrumentation Proteomics - methods Staining and Labeling - instrumentation Staining and Labeling - methods Xenograft Model Antitumor Assays - instrumentation Xenograft Model Antitumor Assays - methods |
| title | Liposome-Assisted Metabolic Glycan Labeling With Cell and Tissue Selectivity |
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