Evaluation of truncated G protein delivered by live attenuated Salmonella as a vaccine against respiratory syncytial virus
Human respiratory syncytial virus (RSV) can cause severe acute lower respiratory tract disease leading to numerous hospitalizations and deaths in the infant and elderly populations worldwide, while no vaccine or effective drug is available for RSV infections. In the present study, truncated G protei...
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| Veröffentlicht in: | Microbial pathogenesis 2018-02, Vol.115, p.299-303 |
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| creator | Tian, Puyuan Xu, Di Huang, Zhixiang Meng, Fanjing Fu, Jinheng Wei, Hua Chen, Tingtao |
| description | Human respiratory syncytial virus (RSV) can cause severe acute lower respiratory tract disease leading to numerous hospitalizations and deaths in the infant and elderly populations worldwide, while no vaccine or effective drug is available for RSV infections. In the present study, truncated G protein was successfully expressed both in prokaryotic and eukaryotic system, and high levels of serum IgG in response to truncated G protein were observed both in GD-protein group (intramuscularly with purified GD protein) and GD-VNP20009 group (challenged via the oral route with 1 × 109 CFU of pLIVE-RSV-GD-VNP20009 strains) since 21th day, and GD-VNP20009 significantly reduced the productions of IL-1β, IL-6, and TNF-α, histamine and pathological features caused by the RSV Long strain (P |
| doi_str_mv | 10.1016/j.micpath.2017.12.080 |
| format | Article |
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•Human respiratory syncytial virus (RSV) cause severe acute lower respiratory tract disease and effective drug is available for this infection.•In the present study, we first constructed the pLIVE-RSV-GD-VNP20009 strain to cure the RSV infection using mice model, and the engineering strain can significantly reduce the productions of inflammatory factors, histamine and pathological features caused by the RSV Long strain, indicating Salmonella typhimurium can be used to deliver truncated G DNA vaccine and represents a promising effect to protect host against RSV.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2017.12.080</identifier><identifier>PMID: 29306006</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Constructed DNA vaccine ; Respiratory syncytial virus (RSV) ; VNP20009</subject><ispartof>Microbial pathogenesis, 2018-02, Vol.115, p.299-303</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-be71ae5e5f86fe98ad99afda5502b6354e96abacf579592ddb842a69bf904bd33</citedby><cites>FETCH-LOGICAL-c365t-be71ae5e5f86fe98ad99afda5502b6354e96abacf579592ddb842a69bf904bd33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micpath.2017.12.080$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29306006$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tian, Puyuan</creatorcontrib><creatorcontrib>Xu, Di</creatorcontrib><creatorcontrib>Huang, Zhixiang</creatorcontrib><creatorcontrib>Meng, Fanjing</creatorcontrib><creatorcontrib>Fu, Jinheng</creatorcontrib><creatorcontrib>Wei, Hua</creatorcontrib><creatorcontrib>Chen, Tingtao</creatorcontrib><title>Evaluation of truncated G protein delivered by live attenuated Salmonella as a vaccine against respiratory syncytial virus</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>Human respiratory syncytial virus (RSV) can cause severe acute lower respiratory tract disease leading to numerous hospitalizations and deaths in the infant and elderly populations worldwide, while no vaccine or effective drug is available for RSV infections. In the present study, truncated G protein was successfully expressed both in prokaryotic and eukaryotic system, and high levels of serum IgG in response to truncated G protein were observed both in GD-protein group (intramuscularly with purified GD protein) and GD-VNP20009 group (challenged via the oral route with 1 × 109 CFU of pLIVE-RSV-GD-VNP20009 strains) since 21th day, and GD-VNP20009 significantly reduced the productions of IL-1β, IL-6, and TNF-α, histamine and pathological features caused by the RSV Long strain (P < .01). Our data indicated that Salmonella typhimurium can be used to deliver truncated G DNA vaccine and represents a promising effect to protect host against RSV.
•Human respiratory syncytial virus (RSV) cause severe acute lower respiratory tract disease and effective drug is available for this infection.•In the present study, we first constructed the pLIVE-RSV-GD-VNP20009 strain to cure the RSV infection using mice model, and the engineering strain can significantly reduce the productions of inflammatory factors, histamine and pathological features caused by the RSV Long strain, indicating Salmonella typhimurium can be used to deliver truncated G DNA vaccine and represents a promising effect to protect host against RSV.</description><subject>Constructed DNA vaccine</subject><subject>Respiratory syncytial virus (RSV)</subject><subject>VNP20009</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqFkE1v1DAQQC0EotvCTwD5yCVh7MTZ-IRQVUqlShyAszWxJ-BV4iy2Eyn99WTZhSunGY3efD3G3ggoBYjm_aEcvT1i_llKEPtSyBJaeMZ2AnRTCAntc7aDtpVFDQKu2HVKBwDQdaVfsiupK2gAmh17ultwmDH7KfCp5znOwWImx-_5MU6ZfOCOBr9Q3Grdyk8px5wpzH-wrziMU6BhQI6JI1_QWh825Af6kDKPlI4-Yp7iytMa7Jo9DnzxcU6v2Iseh0SvL_GGff909-32c_H45f7h9uNjYatG5aKjvUBSpPq26Um36LTG3qFSILumUjXpBju0vdprpaVzXVtLbHTXa6g7V1U37N157vbQr5lSNqNP9nRyoGlORuhWq0pXe7Gh6ozaOKUUqTfH6EeMqxFgTtrNwVy0m5N2I6TZtG99by8r5m4k96_rr-cN-HAGaHt08RRNsp6CJecj2Wzc5P-z4jdh7Jns</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Tian, Puyuan</creator><creator>Xu, Di</creator><creator>Huang, Zhixiang</creator><creator>Meng, Fanjing</creator><creator>Fu, Jinheng</creator><creator>Wei, Hua</creator><creator>Chen, Tingtao</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180201</creationdate><title>Evaluation of truncated G protein delivered by live attenuated Salmonella as a vaccine against respiratory syncytial virus</title><author>Tian, Puyuan ; Xu, Di ; Huang, Zhixiang ; Meng, Fanjing ; Fu, Jinheng ; Wei, Hua ; Chen, Tingtao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-be71ae5e5f86fe98ad99afda5502b6354e96abacf579592ddb842a69bf904bd33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Constructed DNA vaccine</topic><topic>Respiratory syncytial virus (RSV)</topic><topic>VNP20009</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tian, Puyuan</creatorcontrib><creatorcontrib>Xu, Di</creatorcontrib><creatorcontrib>Huang, Zhixiang</creatorcontrib><creatorcontrib>Meng, Fanjing</creatorcontrib><creatorcontrib>Fu, Jinheng</creatorcontrib><creatorcontrib>Wei, Hua</creatorcontrib><creatorcontrib>Chen, Tingtao</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tian, Puyuan</au><au>Xu, Di</au><au>Huang, Zhixiang</au><au>Meng, Fanjing</au><au>Fu, Jinheng</au><au>Wei, Hua</au><au>Chen, Tingtao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of truncated G protein delivered by live attenuated Salmonella as a vaccine against respiratory syncytial virus</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2018-02-01</date><risdate>2018</risdate><volume>115</volume><spage>299</spage><epage>303</epage><pages>299-303</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>Human respiratory syncytial virus (RSV) can cause severe acute lower respiratory tract disease leading to numerous hospitalizations and deaths in the infant and elderly populations worldwide, while no vaccine or effective drug is available for RSV infections. In the present study, truncated G protein was successfully expressed both in prokaryotic and eukaryotic system, and high levels of serum IgG in response to truncated G protein were observed both in GD-protein group (intramuscularly with purified GD protein) and GD-VNP20009 group (challenged via the oral route with 1 × 109 CFU of pLIVE-RSV-GD-VNP20009 strains) since 21th day, and GD-VNP20009 significantly reduced the productions of IL-1β, IL-6, and TNF-α, histamine and pathological features caused by the RSV Long strain (P < .01). Our data indicated that Salmonella typhimurium can be used to deliver truncated G DNA vaccine and represents a promising effect to protect host against RSV.
•Human respiratory syncytial virus (RSV) cause severe acute lower respiratory tract disease and effective drug is available for this infection.•In the present study, we first constructed the pLIVE-RSV-GD-VNP20009 strain to cure the RSV infection using mice model, and the engineering strain can significantly reduce the productions of inflammatory factors, histamine and pathological features caused by the RSV Long strain, indicating Salmonella typhimurium can be used to deliver truncated G DNA vaccine and represents a promising effect to protect host against RSV.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>29306006</pmid><doi>10.1016/j.micpath.2017.12.080</doi><tpages>5</tpages></addata></record> |
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| subjects | Constructed DNA vaccine Respiratory syncytial virus (RSV) VNP20009 |
| title | Evaluation of truncated G protein delivered by live attenuated Salmonella as a vaccine against respiratory syncytial virus |
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