Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation
Neural stem (NS) cell lines may be derived via differentiation of pluripotent embryonic stem (ES) cells or from foetal forebrain. However, because NS cells arise in vitro from heterogeneous populations their immediate cellular origin remains unclear. We used microarray-based expression profiling to...
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| Veröffentlicht in: | Molecular and cellular neuroscience 2008-07, Vol.38 (3), p.393-403 |
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| creator | Pollard, Steven M. Wallbank, Richard Tomlinson, Simon Grotewold, Lars Smith, Austin |
| description | Neural stem (NS) cell lines may be derived via differentiation of pluripotent embryonic stem (ES) cells or from foetal forebrain. However, because NS cells arise in vitro from heterogeneous populations their immediate cellular origin remains unclear. We used microarray-based expression profiling to identify a set of markers expressed by mouse NS cells but not ES cells. One differentially expressed gene encodes the cell surface protein, CD44. CD44 expression is activated by FGF-2 in a subset of cells in both differentiating ES cells and foetal forebrain cultures. Following isolation by flow cytometry the CD44
+ population was found to be highly enriched for NS cell founders. We found that other NS cell marker genes are also induced by FGF in culture, including: Adam12, Cadherin20, Cx3cl1, EGFR, Frizzled9, Kitl, Olig1, Olig2 and Vav3. We speculate that the self-renewing NS cell state may be generated in vitro following transcriptional resetting induced by FGF. |
| doi_str_mv | 10.1016/j.mcn.2008.03.012 |
| format | Article |
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+ population was found to be highly enriched for NS cell founders. We found that other NS cell marker genes are also induced by FGF in culture, including: Adam12, Cadherin20, Cx3cl1, EGFR, Frizzled9, Kitl, Olig1, Olig2 and Vav3. We speculate that the self-renewing NS cell state may be generated in vitro following transcriptional resetting induced by FGF.</description><identifier>ISSN: 1044-7431</identifier><identifier>EISSN: 1095-9327</identifier><identifier>DOI: 10.1016/j.mcn.2008.03.012</identifier><identifier>PMID: 18504136</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; CD44 ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; Cell Line ; Cells, Cultured ; Embryonic stem cell ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - drug effects ; Embryonic Stem Cells - physiology ; Epidermal growth factor ; Female ; Fibroblast growth factor ; Fibroblast Growth Factors - pharmacology ; Flow cytometry ; Genetic Markers - drug effects ; Genetic Markers - physiology ; Mice ; Mice, Inbred C57BL ; Mice, Inbred CBA ; Microarray ; Mouse ; Neural stem cell ; Neurons - cytology ; Neurons - drug effects ; Neurons - physiology ; Phenotype ; Pregnancy ; Prosencephalon - cytology ; Prosencephalon - drug effects ; Prosencephalon - physiology</subject><ispartof>Molecular and cellular neuroscience, 2008-07, Vol.38 (3), p.393-403</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-7a512f38ef2a76d410da64cee07693262336ebce1b3f0f9169933ad41d0b4ddf3</citedby><cites>FETCH-LOGICAL-c382t-7a512f38ef2a76d410da64cee07693262336ebce1b3f0f9169933ad41d0b4ddf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mcn.2008.03.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18504136$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pollard, Steven M.</creatorcontrib><creatorcontrib>Wallbank, Richard</creatorcontrib><creatorcontrib>Tomlinson, Simon</creatorcontrib><creatorcontrib>Grotewold, Lars</creatorcontrib><creatorcontrib>Smith, Austin</creatorcontrib><title>Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation</title><title>Molecular and cellular neuroscience</title><addtitle>Mol Cell Neurosci</addtitle><description>Neural stem (NS) cell lines may be derived via differentiation of pluripotent embryonic stem (ES) cells or from foetal forebrain. However, because NS cells arise in vitro from heterogeneous populations their immediate cellular origin remains unclear. We used microarray-based expression profiling to identify a set of markers expressed by mouse NS cells but not ES cells. One differentially expressed gene encodes the cell surface protein, CD44. CD44 expression is activated by FGF-2 in a subset of cells in both differentiating ES cells and foetal forebrain cultures. Following isolation by flow cytometry the CD44
+ population was found to be highly enriched for NS cell founders. We found that other NS cell marker genes are also induced by FGF in culture, including: Adam12, Cadherin20, Cx3cl1, EGFR, Frizzled9, Kitl, Olig1, Olig2 and Vav3. We speculate that the self-renewing NS cell state may be generated in vitro following transcriptional resetting induced by FGF.</description><subject>Animals</subject><subject>CD44</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Embryonic stem cell</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - drug effects</subject><subject>Embryonic Stem Cells - physiology</subject><subject>Epidermal growth factor</subject><subject>Female</subject><subject>Fibroblast growth factor</subject><subject>Fibroblast Growth Factors - pharmacology</subject><subject>Flow cytometry</subject><subject>Genetic Markers - drug effects</subject><subject>Genetic Markers - physiology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred CBA</subject><subject>Microarray</subject><subject>Mouse</subject><subject>Neural stem cell</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>Neurons - physiology</subject><subject>Phenotype</subject><subject>Pregnancy</subject><subject>Prosencephalon - cytology</subject><subject>Prosencephalon - drug effects</subject><subject>Prosencephalon - physiology</subject><issn>1044-7431</issn><issn>1095-9327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9q3DAQxkVJyP8H6KXo1JvdkeW1LXIKoWkKgV6as5Cl0UaLLW0lOWHfIQ9dLbvQnnLSoPl9HzPzEfKZQc2Add829ax93QAMNfAaWPOJXDAQq0rwpj_Z121b9S1n5-QypQ0ArBrBz8g5G1bQMt5dkPcHN8YwTipluo7hLb9Qq3QOkTpvFo2JKupxiWqiKeNMNU4T3b6gD3m3xQJRGzCXrg0Rx6jKxzaGNXpXPIrYG2qW6Pya4jzGXfBO_2dknLUY0Wensgv-mpxaNSW8Ob5X5Pnh--_7x-rp14-f93dPleZDk6terVhj-YC2UX1nWgZGda1GhL4rm3cN5x2OGtnILVjBOiE4V4UzMLbGWH5Fvh58y6h_FkxZzi7tB1Iew5IkE4PgohUFZAdQx5BSRCu30c0q7iQDuY9AbmSJQO4jkMBliaBovhzNl3FG809xvHkBbg8AlhVfHUaZtEOv0biIOksT3Af2fwH7XJqm</recordid><startdate>20080701</startdate><enddate>20080701</enddate><creator>Pollard, Steven M.</creator><creator>Wallbank, Richard</creator><creator>Tomlinson, Simon</creator><creator>Grotewold, Lars</creator><creator>Smith, Austin</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20080701</creationdate><title>Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation</title><author>Pollard, Steven M. ; Wallbank, Richard ; Tomlinson, Simon ; Grotewold, Lars ; Smith, Austin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-7a512f38ef2a76d410da64cee07693262336ebce1b3f0f9169933ad41d0b4ddf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>CD44</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>Embryonic stem cell</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - drug effects</topic><topic>Embryonic Stem Cells - physiology</topic><topic>Epidermal growth factor</topic><topic>Female</topic><topic>Fibroblast growth factor</topic><topic>Fibroblast Growth Factors - pharmacology</topic><topic>Flow cytometry</topic><topic>Genetic Markers - drug effects</topic><topic>Genetic Markers - physiology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred CBA</topic><topic>Microarray</topic><topic>Mouse</topic><topic>Neural stem cell</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>Neurons - physiology</topic><topic>Phenotype</topic><topic>Pregnancy</topic><topic>Prosencephalon - cytology</topic><topic>Prosencephalon - drug effects</topic><topic>Prosencephalon - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pollard, Steven M.</creatorcontrib><creatorcontrib>Wallbank, Richard</creatorcontrib><creatorcontrib>Tomlinson, Simon</creatorcontrib><creatorcontrib>Grotewold, Lars</creatorcontrib><creatorcontrib>Smith, Austin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular and cellular neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pollard, Steven M.</au><au>Wallbank, Richard</au><au>Tomlinson, Simon</au><au>Grotewold, Lars</au><au>Smith, Austin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation</atitle><jtitle>Molecular and cellular neuroscience</jtitle><addtitle>Mol Cell Neurosci</addtitle><date>2008-07-01</date><risdate>2008</risdate><volume>38</volume><issue>3</issue><spage>393</spage><epage>403</epage><pages>393-403</pages><issn>1044-7431</issn><eissn>1095-9327</eissn><abstract>Neural stem (NS) cell lines may be derived via differentiation of pluripotent embryonic stem (ES) cells or from foetal forebrain. However, because NS cells arise in vitro from heterogeneous populations their immediate cellular origin remains unclear. We used microarray-based expression profiling to identify a set of markers expressed by mouse NS cells but not ES cells. One differentially expressed gene encodes the cell surface protein, CD44. CD44 expression is activated by FGF-2 in a subset of cells in both differentiating ES cells and foetal forebrain cultures. Following isolation by flow cytometry the CD44
+ population was found to be highly enriched for NS cell founders. We found that other NS cell marker genes are also induced by FGF in culture, including: Adam12, Cadherin20, Cx3cl1, EGFR, Frizzled9, Kitl, Olig1, Olig2 and Vav3. We speculate that the self-renewing NS cell state may be generated in vitro following transcriptional resetting induced by FGF.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18504136</pmid><doi>10.1016/j.mcn.2008.03.012</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals CD44 Cell Differentiation - drug effects Cell Differentiation - physiology Cell Line Cells, Cultured Embryonic stem cell Embryonic Stem Cells - cytology Embryonic Stem Cells - drug effects Embryonic Stem Cells - physiology Epidermal growth factor Female Fibroblast growth factor Fibroblast Growth Factors - pharmacology Flow cytometry Genetic Markers - drug effects Genetic Markers - physiology Mice Mice, Inbred C57BL Mice, Inbred CBA Microarray Mouse Neural stem cell Neurons - cytology Neurons - drug effects Neurons - physiology Phenotype Pregnancy Prosencephalon - cytology Prosencephalon - drug effects Prosencephalon - physiology |
| title | Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation |
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