cDNA cloning, characterization, and expression analysis of channel catfish (Ictalurus punctatus Rafinesque, 1818) Peroxiredoxin 6 gene
The peroxiredoxin 6 gene (Prdx6) of channel catfish, Ictalurus punctatus, has been cloned and characterized. The sequence of the channel catfish Prdx6 gene consists of 1,003 nucleotides. Analysis of the nucleotide sequence reveals one open reading frame and 55-bp 5' and 276-bp 3'-untransla...
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| Veröffentlicht in: | Fish physiology and biochemistry 2007-09, Vol.33 (3), p.233-239 |
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| creator | Yeh, Hung-Yueh Klesius, Phillip H |
| description | The peroxiredoxin 6 gene (Prdx6) of channel catfish, Ictalurus punctatus, has been cloned and characterized. The sequence of the channel catfish Prdx6 gene consists of 1,003 nucleotides. Analysis of the nucleotide sequence reveals one open reading frame and 55-bp 5' and 276-bp 3'-untranslated regions. The open reading frame potentially encodes 223 amino acids with a calculated molecular mass of 24.6 kDa. The amino acid sequence of channel catfish Prdx6 had 85.2%, 77.7%, 74.1%, 73.7%, 73.7%, 73.7%, 73.2%, and 71.9% identity with that of zebrafish, chicken, human, mouse, Norway rat, pig, cow, and Xenopus laevis, respectively. Channel catfish Prdx6 had four cysteine residues. In addition to one conserved at Cys46, the Cys132 residue seems to be conserved among fish and frog. Channel catfish Prdx6 conserved two reactive motifs with peroxidase and phopholipase A₂ activity. The channel catfish Prdx6 transcript was detected in all the tissues analyzed, suggesting the gene is constitutively expressed and is involved in protecting channel catfish tissues from oxidative damage. This result will not only provide information for further exploration of the role of Prdx6 in fish pathophysiology but also fill the gap in the evolutionary tree. |
| doi_str_mv | 10.1007/s10695-007-9135-8 |
| format | Article |
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The sequence of the channel catfish Prdx6 gene consists of 1,003 nucleotides. Analysis of the nucleotide sequence reveals one open reading frame and 55-bp 5' and 276-bp 3'-untranslated regions. The open reading frame potentially encodes 223 amino acids with a calculated molecular mass of 24.6 kDa. The amino acid sequence of channel catfish Prdx6 had 85.2%, 77.7%, 74.1%, 73.7%, 73.7%, 73.7%, 73.2%, and 71.9% identity with that of zebrafish, chicken, human, mouse, Norway rat, pig, cow, and Xenopus laevis, respectively. Channel catfish Prdx6 had four cysteine residues. In addition to one conserved at Cys46, the Cys132 residue seems to be conserved among fish and frog. Channel catfish Prdx6 conserved two reactive motifs with peroxidase and phopholipase A₂ activity. The channel catfish Prdx6 transcript was detected in all the tissues analyzed, suggesting the gene is constitutively expressed and is involved in protecting channel catfish tissues from oxidative damage. This result will not only provide information for further exploration of the role of Prdx6 in fish pathophysiology but also fill the gap in the evolutionary tree.</description><identifier>ISSN: 0920-1742</identifier><identifier>EISSN: 1573-5168</identifier><identifier>DOI: 10.1007/s10695-007-9135-8</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Kluwer Academic Publishers</publisher><subject>3' Untranslated regions ; Amino acid sequence ; Amino acid sequences ; Amino acids ; Antioxidant gene ; Anura ; Aquaculture ; Catfish ; Cloning ; Complementary DNA ; conserved sequences ; Danio rerio ; DNA ; Fish ; Freshwater ; Freshwater fishes ; Ictalurus punctatus ; molecular cloning ; molecular sequence data ; Molecular weight ; Nucleotide sequence ; nucleotide sequences ; Nucleotides ; Peroxidase ; Peroxiredoxin ; Peroxiredoxin 6 ; Prdx6 ; Residues ; sequence analysis ; Sequencing ; Tissue ; tissue distribution ; Xenopus laevis ; Zebrafish</subject><ispartof>Fish physiology and biochemistry, 2007-09, Vol.33 (3), p.233-239</ispartof><rights>Springer Science+Business Media, Inc. 2007.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c327t-a7ef46b55e0b572ae88a52d6fbf389586271ddd19c6847a62c5a579bfdada1593</citedby><cites>FETCH-LOGICAL-c327t-a7ef46b55e0b572ae88a52d6fbf389586271ddd19c6847a62c5a579bfdada1593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Yeh, Hung-Yueh</creatorcontrib><creatorcontrib>Klesius, Phillip H</creatorcontrib><title>cDNA cloning, characterization, and expression analysis of channel catfish (Ictalurus punctatus Rafinesque, 1818) Peroxiredoxin 6 gene</title><title>Fish physiology and biochemistry</title><description>The peroxiredoxin 6 gene (Prdx6) of channel catfish, Ictalurus punctatus, has been cloned and characterized. The sequence of the channel catfish Prdx6 gene consists of 1,003 nucleotides. Analysis of the nucleotide sequence reveals one open reading frame and 55-bp 5' and 276-bp 3'-untranslated regions. The open reading frame potentially encodes 223 amino acids with a calculated molecular mass of 24.6 kDa. The amino acid sequence of channel catfish Prdx6 had 85.2%, 77.7%, 74.1%, 73.7%, 73.7%, 73.7%, 73.2%, and 71.9% identity with that of zebrafish, chicken, human, mouse, Norway rat, pig, cow, and Xenopus laevis, respectively. Channel catfish Prdx6 had four cysteine residues. In addition to one conserved at Cys46, the Cys132 residue seems to be conserved among fish and frog. Channel catfish Prdx6 conserved two reactive motifs with peroxidase and phopholipase A₂ activity. The channel catfish Prdx6 transcript was detected in all the tissues analyzed, suggesting the gene is constitutively expressed and is involved in protecting channel catfish tissues from oxidative damage. This result will not only provide information for further exploration of the role of Prdx6 in fish pathophysiology but also fill the gap in the evolutionary tree.</description><subject>3' Untranslated regions</subject><subject>Amino acid sequence</subject><subject>Amino acid sequences</subject><subject>Amino acids</subject><subject>Antioxidant gene</subject><subject>Anura</subject><subject>Aquaculture</subject><subject>Catfish</subject><subject>Cloning</subject><subject>Complementary DNA</subject><subject>conserved sequences</subject><subject>Danio rerio</subject><subject>DNA</subject><subject>Fish</subject><subject>Freshwater</subject><subject>Freshwater fishes</subject><subject>Ictalurus punctatus</subject><subject>molecular cloning</subject><subject>molecular sequence data</subject><subject>Molecular weight</subject><subject>Nucleotide sequence</subject><subject>nucleotide sequences</subject><subject>Nucleotides</subject><subject>Peroxidase</subject><subject>Peroxiredoxin</subject><subject>Peroxiredoxin 6</subject><subject>Prdx6</subject><subject>Residues</subject><subject>sequence analysis</subject><subject>Sequencing</subject><subject>Tissue</subject><subject>tissue distribution</subject><subject>Xenopus laevis</subject><subject>Zebrafish</subject><issn>0920-1742</issn><issn>1573-5168</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkc9uFDEMxiMEEkvhATgRcUBU2oE42fw7VqVApQoQ0HPkzSTbVNNkSWaklgfguclqOXGxP0s_W7Y_Ql4CeweM6fcNmLJy6HKwIORgHpEVSC0GCco8JitmORtAb_hT8qy1W8aY1QpW5I__8OWM-qnklHdr6m-wop9DTb9xTiWvKeaRhvt9Da31upc4PbTUaIkHOOcwUY9zTO2Gvr30M05LXRrdL7nruavvGFMO7dcS1hQMmFP6LdRyn2oYe8xU0V3I4Tl5EnFq4cW_fEKuP178PP88XH39dHl-djV4wfU8oA5xo7ZSBraVmmMwBiUfVdxGYaw0imsYxxGsV2ajUXEvUWq7jSOOCNKKE_LmOHdfS1-pze4uNR-mCXMoS3NgjRWwER18_R94W5baj2_OmP5gEFx2CI6Qr6W1GqLb13SH9cEBcwdb3NEWd5AHW5zpPa-OPRGLw11NzV3_4AxEZ2xfX4m_IVaKXQ</recordid><startdate>20070901</startdate><enddate>20070901</enddate><creator>Yeh, Hung-Yueh</creator><creator>Klesius, 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data</topic><topic>Molecular weight</topic><topic>Nucleotide sequence</topic><topic>nucleotide sequences</topic><topic>Nucleotides</topic><topic>Peroxidase</topic><topic>Peroxiredoxin</topic><topic>Peroxiredoxin 6</topic><topic>Prdx6</topic><topic>Residues</topic><topic>sequence analysis</topic><topic>Sequencing</topic><topic>Tissue</topic><topic>tissue distribution</topic><topic>Xenopus laevis</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yeh, Hung-Yueh</creatorcontrib><creatorcontrib>Klesius, Phillip H</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oceanic 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H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cDNA cloning, characterization, and expression analysis of channel catfish (Ictalurus punctatus Rafinesque, 1818) Peroxiredoxin 6 gene</atitle><jtitle>Fish physiology and biochemistry</jtitle><date>2007-09-01</date><risdate>2007</risdate><volume>33</volume><issue>3</issue><spage>233</spage><epage>239</epage><pages>233-239</pages><issn>0920-1742</issn><eissn>1573-5168</eissn><abstract>The peroxiredoxin 6 gene (Prdx6) of channel catfish, Ictalurus punctatus, has been cloned and characterized. The sequence of the channel catfish Prdx6 gene consists of 1,003 nucleotides. Analysis of the nucleotide sequence reveals one open reading frame and 55-bp 5' and 276-bp 3'-untranslated regions. The open reading frame potentially encodes 223 amino acids with a calculated molecular mass of 24.6 kDa. The amino acid sequence of channel catfish Prdx6 had 85.2%, 77.7%, 74.1%, 73.7%, 73.7%, 73.7%, 73.2%, and 71.9% identity with that of zebrafish, chicken, human, mouse, Norway rat, pig, cow, and Xenopus laevis, respectively. Channel catfish Prdx6 had four cysteine residues. In addition to one conserved at Cys46, the Cys132 residue seems to be conserved among fish and frog. Channel catfish Prdx6 conserved two reactive motifs with peroxidase and phopholipase A₂ activity. The channel catfish Prdx6 transcript was detected in all the tissues analyzed, suggesting the gene is constitutively expressed and is involved in protecting channel catfish tissues from oxidative damage. This result will not only provide information for further exploration of the role of Prdx6 in fish pathophysiology but also fill the gap in the evolutionary tree.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Kluwer Academic Publishers</pub><doi>10.1007/s10695-007-9135-8</doi><tpages>7</tpages></addata></record> |
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| ispartof | Fish physiology and biochemistry, 2007-09, Vol.33 (3), p.233-239 |
| issn | 0920-1742 1573-5168 |
| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | 3' Untranslated regions Amino acid sequence Amino acid sequences Amino acids Antioxidant gene Anura Aquaculture Catfish Cloning Complementary DNA conserved sequences Danio rerio DNA Fish Freshwater Freshwater fishes Ictalurus punctatus molecular cloning molecular sequence data Molecular weight Nucleotide sequence nucleotide sequences Nucleotides Peroxidase Peroxiredoxin Peroxiredoxin 6 Prdx6 Residues sequence analysis Sequencing Tissue tissue distribution Xenopus laevis Zebrafish |
| title | cDNA cloning, characterization, and expression analysis of channel catfish (Ictalurus punctatus Rafinesque, 1818) Peroxiredoxin 6 gene |
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