Comparison of the l-malic acid production by isolated fumarase and fumarase in permeabilized baker's yeast cells
Production of l-malic acid from fumaric acid was performed by the action of the fumarase isolated from porcine heart and the fumarase in permeabilized baker's yeast cells. For each biocatalyst kinetic parameters were determined by measuring the initial reaction rate, and confirmed in the batch...
Gespeichert in:
| Veröffentlicht in: | Enzyme and microbial technology 2007-10, Vol.41 (5), p.605-612 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 612 |
|---|---|
| container_issue | 5 |
| container_start_page | 605 |
| container_title | Enzyme and microbial technology |
| container_volume | 41 |
| creator | Presecki, Ana Vrsalovic Zelic, Bruno Vasic-Racki, Durda |
| description | Production of
l-malic acid from fumaric acid was performed by the action of the fumarase isolated from porcine heart and the fumarase in permeabilized baker's yeast cells. For each biocatalyst kinetic parameters were determined by measuring the initial reaction rate, and confirmed in the batch experiments. It was found that this reaction is inhibited by the product. Equilibrium conversion of about 80% was achieved in a batch mode by both biocatalysts. No by-product was detected during the
l-malic production by permeabilized yeast cell. It was calculated by using the proposed model and experimentally verifies that 10
mg isolated fumarase gives the identical productivity in this biotransformation as 94.4
g of baker's yeast. |
| doi_str_mv | 10.1016/j.enzmictec.2007.05.007 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19888780</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0141022907001809</els_id><sourcerecordid>19888780</sourcerecordid><originalsourceid>FETCH-LOGICAL-c442t-f7b0f0c9128d011bd1823c62c51c3f48dc0fc33b140c6dcdf2dc8c2850a5bc73</originalsourceid><addsrcrecordid>eNqFkMtqHDEQRUWIIRPH3xBt7Ky6U1K_pKUZ4gcYvPFeqEslokm_IvUYxl8fDWMc77y6lOqUqu5l7LuAUoBof-5Kml7GgCthKQG6Epoyyye2EarTBWjQn9kGRC0KkFJ_YV9T2gHkhxo2bNnO42JjSPPEZ8_X38SHYrRDQG4xOL7E2e1xDbndH3jGBruS434_2mgTcTu9K8LEF4oj2T4M4SVjvf1D8UfiB7Jp5UjDkL6xM2-HRBeves6ebn49be-Kh8fb--31Q4F1LdfCdz14QC2kciBE74SSFbYSG4GVr5VD8FhVvagBW4fOS4cKpWrANj121Tm7On2bDfzdU1rNGNLxADvRvE9GaKVUpyCD3QnEOKcUyZslhuznYASYY8BmZ94CNseADTQmS568fF1hE9rBRzthSP_HldZKt23mrk8cZbvPgaJJGGhCciESrsbN4cNd_wBFLJfn</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19888780</pqid></control><display><type>article</type><title>Comparison of the l-malic acid production by isolated fumarase and fumarase in permeabilized baker's yeast cells</title><source>Elsevier ScienceDirect Journals</source><creator>Presecki, Ana Vrsalovic ; Zelic, Bruno ; Vasic-Racki, Durda</creator><creatorcontrib>Presecki, Ana Vrsalovic ; Zelic, Bruno ; Vasic-Racki, Durda</creatorcontrib><description>Production of
l-malic acid from fumaric acid was performed by the action of the fumarase isolated from porcine heart and the fumarase in permeabilized baker's yeast cells. For each biocatalyst kinetic parameters were determined by measuring the initial reaction rate, and confirmed in the batch experiments. It was found that this reaction is inhibited by the product. Equilibrium conversion of about 80% was achieved in a batch mode by both biocatalysts. No by-product was detected during the
l-malic production by permeabilized yeast cell. It was calculated by using the proposed model and experimentally verifies that 10
mg isolated fumarase gives the identical productivity in this biotransformation as 94.4
g of baker's yeast.</description><identifier>ISSN: 0141-0229</identifier><identifier>EISSN: 1879-0909</identifier><identifier>DOI: 10.1016/j.enzmictec.2007.05.007</identifier><identifier>CODEN: EMTED2</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Baker's yeast ; Biological and medical sciences ; Biotechnology ; Fumarase ; Fundamental and applied biological sciences. Psychology ; l-Malic acid ; Modelling ; Permeabilization ; Saccharomyces cerevisiae</subject><ispartof>Enzyme and microbial technology, 2007-10, Vol.41 (5), p.605-612</ispartof><rights>2007 Elsevier Inc.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-f7b0f0c9128d011bd1823c62c51c3f48dc0fc33b140c6dcdf2dc8c2850a5bc73</citedby><cites>FETCH-LOGICAL-c442t-f7b0f0c9128d011bd1823c62c51c3f48dc0fc33b140c6dcdf2dc8c2850a5bc73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0141022907001809$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18998966$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Presecki, Ana Vrsalovic</creatorcontrib><creatorcontrib>Zelic, Bruno</creatorcontrib><creatorcontrib>Vasic-Racki, Durda</creatorcontrib><title>Comparison of the l-malic acid production by isolated fumarase and fumarase in permeabilized baker's yeast cells</title><title>Enzyme and microbial technology</title><description>Production of
l-malic acid from fumaric acid was performed by the action of the fumarase isolated from porcine heart and the fumarase in permeabilized baker's yeast cells. For each biocatalyst kinetic parameters were determined by measuring the initial reaction rate, and confirmed in the batch experiments. It was found that this reaction is inhibited by the product. Equilibrium conversion of about 80% was achieved in a batch mode by both biocatalysts. No by-product was detected during the
l-malic production by permeabilized yeast cell. It was calculated by using the proposed model and experimentally verifies that 10
mg isolated fumarase gives the identical productivity in this biotransformation as 94.4
g of baker's yeast.</description><subject>Baker's yeast</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fumarase</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>l-Malic acid</subject><subject>Modelling</subject><subject>Permeabilization</subject><subject>Saccharomyces cerevisiae</subject><issn>0141-0229</issn><issn>1879-0909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkMtqHDEQRUWIIRPH3xBt7Ky6U1K_pKUZ4gcYvPFeqEslokm_IvUYxl8fDWMc77y6lOqUqu5l7LuAUoBof-5Kml7GgCthKQG6Epoyyye2EarTBWjQn9kGRC0KkFJ_YV9T2gHkhxo2bNnO42JjSPPEZ8_X38SHYrRDQG4xOL7E2e1xDbndH3jGBruS434_2mgTcTu9K8LEF4oj2T4M4SVjvf1D8UfiB7Jp5UjDkL6xM2-HRBeves6ebn49be-Kh8fb--31Q4F1LdfCdz14QC2kciBE74SSFbYSG4GVr5VD8FhVvagBW4fOS4cKpWrANj121Tm7On2bDfzdU1rNGNLxADvRvE9GaKVUpyCD3QnEOKcUyZslhuznYASYY8BmZ94CNseADTQmS568fF1hE9rBRzthSP_HldZKt23mrk8cZbvPgaJJGGhCciESrsbN4cNd_wBFLJfn</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>Presecki, Ana Vrsalovic</creator><creator>Zelic, Bruno</creator><creator>Vasic-Racki, Durda</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20071001</creationdate><title>Comparison of the l-malic acid production by isolated fumarase and fumarase in permeabilized baker's yeast cells</title><author>Presecki, Ana Vrsalovic ; Zelic, Bruno ; Vasic-Racki, Durda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-f7b0f0c9128d011bd1823c62c51c3f48dc0fc33b140c6dcdf2dc8c2850a5bc73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Baker's yeast</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Fumarase</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>l-Malic acid</topic><topic>Modelling</topic><topic>Permeabilization</topic><topic>Saccharomyces cerevisiae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Presecki, Ana Vrsalovic</creatorcontrib><creatorcontrib>Zelic, Bruno</creatorcontrib><creatorcontrib>Vasic-Racki, Durda</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Enzyme and microbial technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Presecki, Ana Vrsalovic</au><au>Zelic, Bruno</au><au>Vasic-Racki, Durda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of the l-malic acid production by isolated fumarase and fumarase in permeabilized baker's yeast cells</atitle><jtitle>Enzyme and microbial technology</jtitle><date>2007-10-01</date><risdate>2007</risdate><volume>41</volume><issue>5</issue><spage>605</spage><epage>612</epage><pages>605-612</pages><issn>0141-0229</issn><eissn>1879-0909</eissn><coden>EMTED2</coden><abstract>Production of
l-malic acid from fumaric acid was performed by the action of the fumarase isolated from porcine heart and the fumarase in permeabilized baker's yeast cells. For each biocatalyst kinetic parameters were determined by measuring the initial reaction rate, and confirmed in the batch experiments. It was found that this reaction is inhibited by the product. Equilibrium conversion of about 80% was achieved in a batch mode by both biocatalysts. No by-product was detected during the
l-malic production by permeabilized yeast cell. It was calculated by using the proposed model and experimentally verifies that 10
mg isolated fumarase gives the identical productivity in this biotransformation as 94.4
g of baker's yeast.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><doi>10.1016/j.enzmictec.2007.05.007</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-0229 |
| ispartof | Enzyme and microbial technology, 2007-10, Vol.41 (5), p.605-612 |
| issn | 0141-0229 1879-0909 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19888780 |
| source | Elsevier ScienceDirect Journals |
| subjects | Baker's yeast Biological and medical sciences Biotechnology Fumarase Fundamental and applied biological sciences. Psychology l-Malic acid Modelling Permeabilization Saccharomyces cerevisiae |
| title | Comparison of the l-malic acid production by isolated fumarase and fumarase in permeabilized baker's yeast cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T05%3A47%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20the%20l-malic%20acid%20production%20by%20isolated%20fumarase%20and%20fumarase%20in%20permeabilized%20baker's%20yeast%20cells&rft.jtitle=Enzyme%20and%20microbial%20technology&rft.au=Presecki,%20Ana%20Vrsalovic&rft.date=2007-10-01&rft.volume=41&rft.issue=5&rft.spage=605&rft.epage=612&rft.pages=605-612&rft.issn=0141-0229&rft.eissn=1879-0909&rft.coden=EMTED2&rft_id=info:doi/10.1016/j.enzmictec.2007.05.007&rft_dat=%3Cproquest_cross%3E19888780%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19888780&rft_id=info:pmid/&rft_els_id=S0141022907001809&rfr_iscdi=true |