cDNA cloning, sequence analysis and expression profiling of HaKettin1 gene from Helicoverpa armigera
[Objective] To clone the full length cDNA sequence of Kettin gene from Helicoverpa armigera and analyze its expression profiles in the life cycle of H. armigera development. [Methods] Using multiple RT-PCR and 5'-, 3'-RACE to clone the full length cDNA sequence of HaKettin1, and semi-quant...
Gespeichert in:
| Veröffentlicht in: | K'un ch'ung hsueh pao 2008-03, Vol.51 (3), p.258-263 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | chi |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 263 |
|---|---|
| container_issue | 3 |
| container_start_page | 258 |
| container_title | K'un ch'ung hsueh pao |
| container_volume | 51 |
| creator | Qi, X-P Niu, B-L He, L-H Weng, H-B Liu, Y Shen, W-F Meng, Z-Q |
| description | [Objective] To clone the full length cDNA sequence of Kettin gene from Helicoverpa armigera and analyze its expression profiles in the life cycle of H. armigera development. [Methods] Using multiple RT-PCR and 5'-, 3'-RACE to clone the full length cDNA sequence of HaKettin1, and semi-quantitative RT-PCR to analyze the expression profiles. [Results] The full length of HaKettin1 cDNA sequence is 13 805 bp, containing an open reading frame of 13 365 bp. The deduced amino acid sequence consists of 4 454 amino acids, with molecular mass about 504.3 kD. The results of expression profiles showed HaKettin1 was expressed in all the cycle of insect life, and the expression of HaKettin1 at larval stage was higher than at other developmental stages. [Conclusions] Percent identity of HaKettin1 protein and Bombyx mori Kettin protein is 90%, suggesting that Kettin proteins are much conserved among Lepidoptera insects. The results of expression profiles suggest that HaKettin1 plays an important role in the development of H. |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_19883231</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>19883231</sourcerecordid><originalsourceid>FETCH-proquest_miscellaneous_198832313</originalsourceid><addsrcrecordid>eNqNjb0KwjAUhTMoWLTvcCcnC-mPJR3FHwqCk3uJ6W0JpEnNbUXf3gw-gNM5w_eds2ARL_ZFUmZVuWIxkX5wzkUpBM8j1qrT7QDKOKttvwPC54xWIUgrzYc0hdICvkePQXQWRu86bQILroNaXnGatE2hR4vQeTdAjUYr90I_SpB-0D16uWHLThrC-Jdrtr2c78c6CWvhj6Zm0KTQGGnRzdSklRB5lqf53-AXEIZJww</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19883231</pqid></control><display><type>article</type><title>cDNA cloning, sequence analysis and expression profiling of HaKettin1 gene from Helicoverpa armigera</title><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Qi, X-P ; Niu, B-L ; He, L-H ; Weng, H-B ; Liu, Y ; Shen, W-F ; Meng, Z-Q</creator><creatorcontrib>Qi, X-P ; Niu, B-L ; He, L-H ; Weng, H-B ; Liu, Y ; Shen, W-F ; Meng, Z-Q</creatorcontrib><description>[Objective] To clone the full length cDNA sequence of Kettin gene from Helicoverpa armigera and analyze its expression profiles in the life cycle of H. armigera development. [Methods] Using multiple RT-PCR and 5'-, 3'-RACE to clone the full length cDNA sequence of HaKettin1, and semi-quantitative RT-PCR to analyze the expression profiles. [Results] The full length of HaKettin1 cDNA sequence is 13 805 bp, containing an open reading frame of 13 365 bp. The deduced amino acid sequence consists of 4 454 amino acids, with molecular mass about 504.3 kD. The results of expression profiles showed HaKettin1 was expressed in all the cycle of insect life, and the expression of HaKettin1 at larval stage was higher than at other developmental stages. [Conclusions] Percent identity of HaKettin1 protein and Bombyx mori Kettin protein is 90%, suggesting that Kettin proteins are much conserved among Lepidoptera insects. The results of expression profiles suggest that HaKettin1 plays an important role in the development of H.</description><identifier>ISSN: 0454-6296</identifier><language>chi</language><subject>Bombyx mori ; Helicoverpa armigera ; Lepidoptera</subject><ispartof>K'un ch'ung hsueh pao, 2008-03, Vol.51 (3), p.258-263</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Qi, X-P</creatorcontrib><creatorcontrib>Niu, B-L</creatorcontrib><creatorcontrib>He, L-H</creatorcontrib><creatorcontrib>Weng, H-B</creatorcontrib><creatorcontrib>Liu, Y</creatorcontrib><creatorcontrib>Shen, W-F</creatorcontrib><creatorcontrib>Meng, Z-Q</creatorcontrib><title>cDNA cloning, sequence analysis and expression profiling of HaKettin1 gene from Helicoverpa armigera</title><title>K'un ch'ung hsueh pao</title><description>[Objective] To clone the full length cDNA sequence of Kettin gene from Helicoverpa armigera and analyze its expression profiles in the life cycle of H. armigera development. [Methods] Using multiple RT-PCR and 5'-, 3'-RACE to clone the full length cDNA sequence of HaKettin1, and semi-quantitative RT-PCR to analyze the expression profiles. [Results] The full length of HaKettin1 cDNA sequence is 13 805 bp, containing an open reading frame of 13 365 bp. The deduced amino acid sequence consists of 4 454 amino acids, with molecular mass about 504.3 kD. The results of expression profiles showed HaKettin1 was expressed in all the cycle of insect life, and the expression of HaKettin1 at larval stage was higher than at other developmental stages. [Conclusions] Percent identity of HaKettin1 protein and Bombyx mori Kettin protein is 90%, suggesting that Kettin proteins are much conserved among Lepidoptera insects. The results of expression profiles suggest that HaKettin1 plays an important role in the development of H.</description><subject>Bombyx mori</subject><subject>Helicoverpa armigera</subject><subject>Lepidoptera</subject><issn>0454-6296</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqNjb0KwjAUhTMoWLTvcCcnC-mPJR3FHwqCk3uJ6W0JpEnNbUXf3gw-gNM5w_eds2ARL_ZFUmZVuWIxkX5wzkUpBM8j1qrT7QDKOKttvwPC54xWIUgrzYc0hdICvkePQXQWRu86bQILroNaXnGatE2hR4vQeTdAjUYr90I_SpB-0D16uWHLThrC-Jdrtr2c78c6CWvhj6Zm0KTQGGnRzdSklRB5lqf53-AXEIZJww</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Qi, X-P</creator><creator>Niu, B-L</creator><creator>He, L-H</creator><creator>Weng, H-B</creator><creator>Liu, Y</creator><creator>Shen, W-F</creator><creator>Meng, Z-Q</creator><scope>7SS</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20080301</creationdate><title>cDNA cloning, sequence analysis and expression profiling of HaKettin1 gene from Helicoverpa armigera</title><author>Qi, X-P ; Niu, B-L ; He, L-H ; Weng, H-B ; Liu, Y ; Shen, W-F ; Meng, Z-Q</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_198832313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2008</creationdate><topic>Bombyx mori</topic><topic>Helicoverpa armigera</topic><topic>Lepidoptera</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qi, X-P</creatorcontrib><creatorcontrib>Niu, B-L</creatorcontrib><creatorcontrib>He, L-H</creatorcontrib><creatorcontrib>Weng, H-B</creatorcontrib><creatorcontrib>Liu, Y</creatorcontrib><creatorcontrib>Shen, W-F</creatorcontrib><creatorcontrib>Meng, Z-Q</creatorcontrib><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>K'un ch'ung hsueh pao</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qi, X-P</au><au>Niu, B-L</au><au>He, L-H</au><au>Weng, H-B</au><au>Liu, Y</au><au>Shen, W-F</au><au>Meng, Z-Q</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cDNA cloning, sequence analysis and expression profiling of HaKettin1 gene from Helicoverpa armigera</atitle><jtitle>K'un ch'ung hsueh pao</jtitle><date>2008-03-01</date><risdate>2008</risdate><volume>51</volume><issue>3</issue><spage>258</spage><epage>263</epage><pages>258-263</pages><issn>0454-6296</issn><abstract>[Objective] To clone the full length cDNA sequence of Kettin gene from Helicoverpa armigera and analyze its expression profiles in the life cycle of H. armigera development. [Methods] Using multiple RT-PCR and 5'-, 3'-RACE to clone the full length cDNA sequence of HaKettin1, and semi-quantitative RT-PCR to analyze the expression profiles. [Results] The full length of HaKettin1 cDNA sequence is 13 805 bp, containing an open reading frame of 13 365 bp. The deduced amino acid sequence consists of 4 454 amino acids, with molecular mass about 504.3 kD. The results of expression profiles showed HaKettin1 was expressed in all the cycle of insect life, and the expression of HaKettin1 at larval stage was higher than at other developmental stages. [Conclusions] Percent identity of HaKettin1 protein and Bombyx mori Kettin protein is 90%, suggesting that Kettin proteins are much conserved among Lepidoptera insects. The results of expression profiles suggest that HaKettin1 plays an important role in the development of H.</abstract></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0454-6296 |
| ispartof | K'un ch'ung hsueh pao, 2008-03, Vol.51 (3), p.258-263 |
| issn | 0454-6296 |
| language | chi |
| recordid | cdi_proquest_miscellaneous_19883231 |
| source | EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Bombyx mori Helicoverpa armigera Lepidoptera |
| title | cDNA cloning, sequence analysis and expression profiling of HaKettin1 gene from Helicoverpa armigera |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T02%3A14%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=cDNA%20cloning,%20sequence%20analysis%20and%20expression%20profiling%20of%20HaKettin1%20gene%20from%20Helicoverpa%20armigera&rft.jtitle=K'un%20ch'ung%20hsueh%20pao&rft.au=Qi,%20X-P&rft.date=2008-03-01&rft.volume=51&rft.issue=3&rft.spage=258&rft.epage=263&rft.pages=258-263&rft.issn=0454-6296&rft_id=info:doi/&rft_dat=%3Cproquest%3E19883231%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19883231&rft_id=info:pmid/&rfr_iscdi=true |