Estrogen Selectively Increases Tryptophan Hydroxylase-2 mRNA Expression in Distinct Subregions of Rat Midbrain Raphe Nucleus: Association between Gene Expression and Anxiety Behavior in the Open Field
Ovarian steroids modulate anxiety behavior, perhaps by regulating the serotonergic neurons in the midbrain raphe nucleus. The regulation of the brain-specific isoform of rat tryptophan hydroxylase (TPH2) by ovarian hormones has not yet been investigated. Therefore, we examined the effects of estroge...
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| Veröffentlicht in: | Biological psychiatry (1969) 2006-08, Vol.60 (3), p.288-295 |
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| creator | Hiroi, Ryoko McDevitt, Ross A. Neumaier, John F. |
| description | Ovarian steroids modulate anxiety behavior, perhaps by regulating the serotonergic neurons in the midbrain raphe nucleus. The regulation of the brain-specific isoform of rat tryptophan hydroxylase (TPH2) by ovarian hormones has not yet been investigated. Therefore, we examined the effects of estrogen and progesterone on TPH2 mRNA in the rat dorsal and median raphe nuclei (DRN and MRN, respectively) and whether TPH2 mRNA levels correlated with anxiety behavior.
Ovariectomized rats were treated for two weeks with placebo, estrogen or estrogen plus progesterone, exposed to the open field test, and TPH2 mRNA was quantified by in situ hybridization histochemistry.
Estrogen increased TPH2 mRNA in the mid-ventromedial and caudal subregions of the DRN and the caudal MRN. Combined estrogen and progesterone treatment did not change TPH2 mRNA relative to ovariectomized controls. TPH2 mRNA in caudal DRN was associated with lower anxiety-like behavior, whereas TPH2 mRNA in rostral dorsomedial DRN was associated with increased anxiety-like behavior.
These results suggest that estrogen may increase the capacity for serotonin synthesis in discrete subgroups of raphe neurons, and reinforce previous observations that different subregions of DRN contribute to distinct components of anxiety behavior. |
| doi_str_mv | 10.1016/j.biopsych.2005.10.019 |
| format | Article |
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Ovariectomized rats were treated for two weeks with placebo, estrogen or estrogen plus progesterone, exposed to the open field test, and TPH2 mRNA was quantified by in situ hybridization histochemistry.
Estrogen increased TPH2 mRNA in the mid-ventromedial and caudal subregions of the DRN and the caudal MRN. Combined estrogen and progesterone treatment did not change TPH2 mRNA relative to ovariectomized controls. TPH2 mRNA in caudal DRN was associated with lower anxiety-like behavior, whereas TPH2 mRNA in rostral dorsomedial DRN was associated with increased anxiety-like behavior.
These results suggest that estrogen may increase the capacity for serotonin synthesis in discrete subgroups of raphe neurons, and reinforce previous observations that different subregions of DRN contribute to distinct components of anxiety behavior.</description><identifier>ISSN: 0006-3223</identifier><identifier>EISSN: 1873-2402</identifier><identifier>DOI: 10.1016/j.biopsych.2005.10.019</identifier><identifier>PMID: 16458260</identifier><identifier>CODEN: BIPCBF</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Anxiety - genetics ; Anxiety - metabolism ; Behavior, Animal ; Behavioral psychophysiology ; Biological and medical sciences ; Densitometry - methods ; dorsal raphe ; Estrogens - pharmacology ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression - genetics ; Gene Expression - physiology ; Immunohistochemistry - methods ; In Situ Hybridization - methods ; median raphe ; Miscellaneous ; Ovarian hormones ; Ovariectomy ; progesterone ; Progesterone - pharmacology ; Psychology. Psychoanalysis. Psychiatry ; Psychology. Psychophysiology ; Raphe Nuclei - drug effects ; Raphe Nuclei - metabolism ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; serotonin ; Spatial Behavior ; TPH2 ; Tryptophan Hydroxylase - biosynthesis ; Tryptophan Hydroxylase - genetics</subject><ispartof>Biological psychiatry (1969), 2006-08, Vol.60 (3), p.288-295</ispartof><rights>2006 Society of Biological Psychiatry</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-ddbfc422be87dff6c52a00f6dfc6a6b2aa4da8cf1023ac457ba53ba9feb68df63</citedby><cites>FETCH-LOGICAL-c462t-ddbfc422be87dff6c52a00f6dfc6a6b2aa4da8cf1023ac457ba53ba9feb68df63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biopsych.2005.10.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18013371$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16458260$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hiroi, Ryoko</creatorcontrib><creatorcontrib>McDevitt, Ross A.</creatorcontrib><creatorcontrib>Neumaier, John F.</creatorcontrib><title>Estrogen Selectively Increases Tryptophan Hydroxylase-2 mRNA Expression in Distinct Subregions of Rat Midbrain Raphe Nucleus: Association between Gene Expression and Anxiety Behavior in the Open Field</title><title>Biological psychiatry (1969)</title><addtitle>Biol Psychiatry</addtitle><description>Ovarian steroids modulate anxiety behavior, perhaps by regulating the serotonergic neurons in the midbrain raphe nucleus. The regulation of the brain-specific isoform of rat tryptophan hydroxylase (TPH2) by ovarian hormones has not yet been investigated. Therefore, we examined the effects of estrogen and progesterone on TPH2 mRNA in the rat dorsal and median raphe nuclei (DRN and MRN, respectively) and whether TPH2 mRNA levels correlated with anxiety behavior.
Ovariectomized rats were treated for two weeks with placebo, estrogen or estrogen plus progesterone, exposed to the open field test, and TPH2 mRNA was quantified by in situ hybridization histochemistry.
Estrogen increased TPH2 mRNA in the mid-ventromedial and caudal subregions of the DRN and the caudal MRN. Combined estrogen and progesterone treatment did not change TPH2 mRNA relative to ovariectomized controls. TPH2 mRNA in caudal DRN was associated with lower anxiety-like behavior, whereas TPH2 mRNA in rostral dorsomedial DRN was associated with increased anxiety-like behavior.
These results suggest that estrogen may increase the capacity for serotonin synthesis in discrete subgroups of raphe neurons, and reinforce previous observations that different subregions of DRN contribute to distinct components of anxiety behavior.</description><subject>Animals</subject><subject>Anxiety - genetics</subject><subject>Anxiety - metabolism</subject><subject>Behavior, Animal</subject><subject>Behavioral psychophysiology</subject><subject>Biological and medical sciences</subject><subject>Densitometry - methods</subject><subject>dorsal raphe</subject><subject>Estrogens - pharmacology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - genetics</subject><subject>Gene Expression - physiology</subject><subject>Immunohistochemistry - methods</subject><subject>In Situ Hybridization - methods</subject><subject>median raphe</subject><subject>Miscellaneous</subject><subject>Ovarian hormones</subject><subject>Ovariectomy</subject><subject>progesterone</subject><subject>Progesterone - pharmacology</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychology. Psychophysiology</subject><subject>Raphe Nuclei - drug effects</subject><subject>Raphe Nuclei - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>serotonin</subject><subject>Spatial Behavior</subject><subject>TPH2</subject><subject>Tryptophan Hydroxylase - biosynthesis</subject><subject>Tryptophan Hydroxylase - genetics</subject><issn>0006-3223</issn><issn>1873-2402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9v0zAUxSMEYmPwFSa_wFuK7aROyhNldH-ksUndeI4c-3p1ldqZr1Oab8jHwlWLxhtPlo9-59yre7LsnNEJo0x8Xk9a63sc1WrCKZ0mcULZ7FV2yuqqyHlJ-evslFIq8oLz4iR7h7hO34pz9jY7YaKc1lzQ0-z3AmPwT-DIA3Sgot1CN5IbpwJIBCSPYeyj71fSketRB78bu6TnnGyWd3Oy2PUBEK13xDry3WK0TkXyMLQBnpKKxBuylJH8sLoNMjFL2a-A3A2qgwG_kDmiV1bGfUIL8RekRa7Awb_J0mkydzsLcSTfYCW31of9uJiC7vtkuLTQ6ffZGyM7hA_H9yz7ebl4vLjOb--vbi7mt7kqBY-51q1RJect1JU2Rqgpl5QaoY0SUrRcylLLWhlGeSFVOa1aOS1aOTPQilobUZxlnw65ffDPA2BsNhYVdJ104Ads2KyuypIWCRQHUAWPGMA0fbAbGcaG0WbfYbNu_nbY7Dvc66nDZDw_ThjaDegX27G0BHw8AhKV7EyQTll84WrKiqJiift64CDdY2shNKgsOAXahlR1o7393y5_AMhgxAs</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>Hiroi, Ryoko</creator><creator>McDevitt, Ross A.</creator><creator>Neumaier, John F.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20060801</creationdate><title>Estrogen Selectively Increases Tryptophan Hydroxylase-2 mRNA Expression in Distinct Subregions of Rat Midbrain Raphe Nucleus: Association between Gene Expression and Anxiety Behavior in the Open Field</title><author>Hiroi, Ryoko ; McDevitt, Ross A. ; Neumaier, John F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-ddbfc422be87dff6c52a00f6dfc6a6b2aa4da8cf1023ac457ba53ba9feb68df63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Anxiety - genetics</topic><topic>Anxiety - metabolism</topic><topic>Behavior, Animal</topic><topic>Behavioral psychophysiology</topic><topic>Biological and medical sciences</topic><topic>Densitometry - methods</topic><topic>dorsal raphe</topic><topic>Estrogens - pharmacology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - genetics</topic><topic>Gene Expression - physiology</topic><topic>Immunohistochemistry - methods</topic><topic>In Situ Hybridization - methods</topic><topic>median raphe</topic><topic>Miscellaneous</topic><topic>Ovarian hormones</topic><topic>Ovariectomy</topic><topic>progesterone</topic><topic>Progesterone - pharmacology</topic><topic>Psychology. Psychoanalysis. Psychiatry</topic><topic>Psychology. Psychophysiology</topic><topic>Raphe Nuclei - drug effects</topic><topic>Raphe Nuclei - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>serotonin</topic><topic>Spatial Behavior</topic><topic>TPH2</topic><topic>Tryptophan Hydroxylase - biosynthesis</topic><topic>Tryptophan Hydroxylase - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hiroi, Ryoko</creatorcontrib><creatorcontrib>McDevitt, Ross A.</creatorcontrib><creatorcontrib>Neumaier, John F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Biological psychiatry (1969)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hiroi, Ryoko</au><au>McDevitt, Ross A.</au><au>Neumaier, John F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Estrogen Selectively Increases Tryptophan Hydroxylase-2 mRNA Expression in Distinct Subregions of Rat Midbrain Raphe Nucleus: Association between Gene Expression and Anxiety Behavior in the Open Field</atitle><jtitle>Biological psychiatry (1969)</jtitle><addtitle>Biol Psychiatry</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>60</volume><issue>3</issue><spage>288</spage><epage>295</epage><pages>288-295</pages><issn>0006-3223</issn><eissn>1873-2402</eissn><coden>BIPCBF</coden><abstract>Ovarian steroids modulate anxiety behavior, perhaps by regulating the serotonergic neurons in the midbrain raphe nucleus. The regulation of the brain-specific isoform of rat tryptophan hydroxylase (TPH2) by ovarian hormones has not yet been investigated. Therefore, we examined the effects of estrogen and progesterone on TPH2 mRNA in the rat dorsal and median raphe nuclei (DRN and MRN, respectively) and whether TPH2 mRNA levels correlated with anxiety behavior.
Ovariectomized rats were treated for two weeks with placebo, estrogen or estrogen plus progesterone, exposed to the open field test, and TPH2 mRNA was quantified by in situ hybridization histochemistry.
Estrogen increased TPH2 mRNA in the mid-ventromedial and caudal subregions of the DRN and the caudal MRN. Combined estrogen and progesterone treatment did not change TPH2 mRNA relative to ovariectomized controls. TPH2 mRNA in caudal DRN was associated with lower anxiety-like behavior, whereas TPH2 mRNA in rostral dorsomedial DRN was associated with increased anxiety-like behavior.
These results suggest that estrogen may increase the capacity for serotonin synthesis in discrete subgroups of raphe neurons, and reinforce previous observations that different subregions of DRN contribute to distinct components of anxiety behavior.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>16458260</pmid><doi>10.1016/j.biopsych.2005.10.019</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biological psychiatry (1969), 2006-08, Vol.60 (3), p.288-295 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Anxiety - genetics Anxiety - metabolism Behavior, Animal Behavioral psychophysiology Biological and medical sciences Densitometry - methods dorsal raphe Estrogens - pharmacology Female Fundamental and applied biological sciences. Psychology Gene Expression - genetics Gene Expression - physiology Immunohistochemistry - methods In Situ Hybridization - methods median raphe Miscellaneous Ovarian hormones Ovariectomy progesterone Progesterone - pharmacology Psychology. Psychoanalysis. Psychiatry Psychology. Psychophysiology Raphe Nuclei - drug effects Raphe Nuclei - metabolism Rats Rats, Sprague-Dawley RNA, Messenger - biosynthesis RNA, Messenger - genetics serotonin Spatial Behavior TPH2 Tryptophan Hydroxylase - biosynthesis Tryptophan Hydroxylase - genetics |
| title | Estrogen Selectively Increases Tryptophan Hydroxylase-2 mRNA Expression in Distinct Subregions of Rat Midbrain Raphe Nucleus: Association between Gene Expression and Anxiety Behavior in the Open Field |
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