Molecular responses during cadmium-induced stress in Daphnia magna: Integration of differential gene expression with higher-level effects
DNA microarrays offer great potential in revealing insight into mechanistic toxicity of contaminants. The aim of the present study was (i) to gain insight in concentration- and time-dependent cadmium-induced molecular responses by using a customized Daphnia magna microarray, and (ii) to compare the...
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| Veröffentlicht in: | Aquatic toxicology 2007-07, Vol.83 (3), p.212-222 |
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| creator | Soetaert, Anneleen Vandenbrouck, Tine van der Ven, Karlijn Maras, Marleen van Remortel, Piet Blust, Ronny De Coen, Wim M. |
| description | DNA microarrays offer great potential in revealing insight into mechanistic toxicity of contaminants. The aim of the present study was (i) to gain insight in concentration- and time-dependent cadmium-induced molecular responses by using a customized
Daphnia magna microarray, and (ii) to compare the gene expression profiles with effects at higher levels of biological organization (e.g. total energy budget and growth). Daphnids were exposed to three cadmium concentrations (nominal value of 10, 50, 100
μg/l) for two time intervals (48 and 96
h). In general, dynamic expression patterns were obtained with a clear increase of gene expression changes at higher concentrations and longer exposure duration. Microarray analysis revealed cadmium affected molecular pathways associated with processes such as digestion, oxygen transport, cuticula metabolism and embryo development. These effects were compared with higher-level effects (energy budgets and growth). For instance, next to reduced energy budgets due to a decline in lipid, carbohydrate and protein content, we found an up-regulated expression of genes related to digestive processes (e.g. α-esterase, cellulase, α-amylase). Furthermore, cadmium affected the expression of genes coding for proteins involved in molecular pathways associated with immune response, stress response, cell adhesion, visual perception and signal transduction in the present study. |
| doi_str_mv | 10.1016/j.aquatox.2007.04.010 |
| format | Article |
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Daphnia magna microarray, and (ii) to compare the gene expression profiles with effects at higher levels of biological organization (e.g. total energy budget and growth). Daphnids were exposed to three cadmium concentrations (nominal value of 10, 50, 100
μg/l) for two time intervals (48 and 96
h). In general, dynamic expression patterns were obtained with a clear increase of gene expression changes at higher concentrations and longer exposure duration. Microarray analysis revealed cadmium affected molecular pathways associated with processes such as digestion, oxygen transport, cuticula metabolism and embryo development. These effects were compared with higher-level effects (energy budgets and growth). For instance, next to reduced energy budgets due to a decline in lipid, carbohydrate and protein content, we found an up-regulated expression of genes related to digestive processes (e.g. α-esterase, cellulase, α-amylase). Furthermore, cadmium affected the expression of genes coding for proteins involved in molecular pathways associated with immune response, stress response, cell adhesion, visual perception and signal transduction in the present study.</description><identifier>ISSN: 0166-445X</identifier><identifier>EISSN: 1879-1514</identifier><identifier>DOI: 10.1016/j.aquatox.2007.04.010</identifier><identifier>PMID: 17582521</identifier><identifier>CODEN: AQTODG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>acid-base balance ; animal cuticle ; animal growth ; Animal, plant and microbial ecology ; Animals ; Applied ecology ; aquatic arthropods ; Biological and medical sciences ; Cadmium ; Cadmium - toxicity ; Cadmium Poisoning - genetics ; Cadmium Poisoning - metabolism ; Daphnia - drug effects ; Daphnia - physiology ; Daphnia magna ; digestion ; dose response ; Dose-Response Relationship, Drug ; ecotoxicogenomics ; Ecotoxicology, biological effects of pollution ; embryogenesis ; energy balance ; Energy budget ; Energy Metabolism ; esterases ; Freshwater ; Fundamental and applied biological sciences. Psychology ; gene expression ; Gene Expression Profiling ; General aspects ; immune response ; metabolism ; Microarray ; microarray technology ; Multiple levels of biological organization ; Oligonucleotide Array Sequence Analysis ; physiological response ; Reverse Transcriptase Polymerase Chain Reaction ; signal transduction ; stress tolerance ; toxicity ; Toxicogenomics ; vision ; vitellogenin</subject><ispartof>Aquatic toxicology, 2007-07, Vol.83 (3), p.212-222</ispartof><rights>2007 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-eb772c6ecf464533c704f38dc56d1b0df5028e6d4ae2b5959212e5ee71d4f8403</citedby><cites>FETCH-LOGICAL-c479t-eb772c6ecf464533c704f38dc56d1b0df5028e6d4ae2b5959212e5ee71d4f8403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aquatox.2007.04.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18893524$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17582521$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soetaert, Anneleen</creatorcontrib><creatorcontrib>Vandenbrouck, Tine</creatorcontrib><creatorcontrib>van der Ven, Karlijn</creatorcontrib><creatorcontrib>Maras, Marleen</creatorcontrib><creatorcontrib>van Remortel, Piet</creatorcontrib><creatorcontrib>Blust, Ronny</creatorcontrib><creatorcontrib>De Coen, Wim M.</creatorcontrib><title>Molecular responses during cadmium-induced stress in Daphnia magna: Integration of differential gene expression with higher-level effects</title><title>Aquatic toxicology</title><addtitle>Aquat Toxicol</addtitle><description>DNA microarrays offer great potential in revealing insight into mechanistic toxicity of contaminants. The aim of the present study was (i) to gain insight in concentration- and time-dependent cadmium-induced molecular responses by using a customized
Daphnia magna microarray, and (ii) to compare the gene expression profiles with effects at higher levels of biological organization (e.g. total energy budget and growth). Daphnids were exposed to three cadmium concentrations (nominal value of 10, 50, 100
μg/l) for two time intervals (48 and 96
h). In general, dynamic expression patterns were obtained with a clear increase of gene expression changes at higher concentrations and longer exposure duration. Microarray analysis revealed cadmium affected molecular pathways associated with processes such as digestion, oxygen transport, cuticula metabolism and embryo development. These effects were compared with higher-level effects (energy budgets and growth). For instance, next to reduced energy budgets due to a decline in lipid, carbohydrate and protein content, we found an up-regulated expression of genes related to digestive processes (e.g. α-esterase, cellulase, α-amylase). Furthermore, cadmium affected the expression of genes coding for proteins involved in molecular pathways associated with immune response, stress response, cell adhesion, visual perception and signal transduction in the present study.</description><subject>acid-base balance</subject><subject>animal cuticle</subject><subject>animal growth</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>aquatic arthropods</subject><subject>Biological and medical sciences</subject><subject>Cadmium</subject><subject>Cadmium - toxicity</subject><subject>Cadmium Poisoning - genetics</subject><subject>Cadmium Poisoning - metabolism</subject><subject>Daphnia - drug effects</subject><subject>Daphnia - physiology</subject><subject>Daphnia magna</subject><subject>digestion</subject><subject>dose response</subject><subject>Dose-Response Relationship, Drug</subject><subject>ecotoxicogenomics</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>embryogenesis</subject><subject>energy balance</subject><subject>Energy budget</subject><subject>Energy Metabolism</subject><subject>esterases</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Gene Expression Profiling</subject><subject>General aspects</subject><subject>immune response</subject><subject>metabolism</subject><subject>Microarray</subject><subject>microarray technology</subject><subject>Multiple levels of biological organization</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>physiological response</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>signal transduction</subject><subject>stress tolerance</subject><subject>toxicity</subject><subject>Toxicogenomics</subject><subject>vision</subject><subject>vitellogenin</subject><issn>0166-445X</issn><issn>1879-1514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U-P1CAYBnBiNO64-hFULnprBQqFejFm_bfJGg-6iTfCwEuHSUtnoV3Xj-C3lsk02eNy4cDvAfI-CL2kpKaEtu_2tblZzDzd1YwQWRNeE0oeoQ1VsquooPwx2hTXVpyL32foWc57Uhbj3VN0RqVQTDC6Qf--TwPYZTAJJ8iHKWbI2C0pxB5b48awjFWIbrHgcJ4LyThE_MkcdjEYPJo-mvf4Ms7QJzOHKeLJYxe8hwRxDmbAPUTAcHc4Ro_nf8K8w7vQ7yBVA9zCgKFoO-fn6Ik3Q4YX636Orr98_nXxrbr68fXy4uNVZbns5gq2UjLbgvW85aJprCTcN8pZ0Tq6Jc4LwhS0jhtgW9GJjlEGAkBSx73ipDlHb0_3HtJ0s0Ce9RiyhWEwEaYla9op2ahOPgy5Im1LmgLFCdo05ZzA60MKo0l_NSX6WJbe67UsfSxLE65LWSX3an1g2Y7g7lNrOwW8WYHJ1gw-mWhDvndKdY1gvLjXJ-fNpE2firn-yQhtCFGcSqWK-HASUCZ7GyDpbAPE0mpIZfjaTeGBz_4Hi-vBsQ</recordid><startdate>20070720</startdate><enddate>20070720</enddate><creator>Soetaert, Anneleen</creator><creator>Vandenbrouck, Tine</creator><creator>van der Ven, Karlijn</creator><creator>Maras, Marleen</creator><creator>van Remortel, Piet</creator><creator>Blust, Ronny</creator><creator>De Coen, Wim M.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7QH</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H97</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20070720</creationdate><title>Molecular responses during cadmium-induced stress in Daphnia magna: Integration of differential gene expression with higher-level effects</title><author>Soetaert, Anneleen ; Vandenbrouck, Tine ; van der Ven, Karlijn ; Maras, Marleen ; van Remortel, Piet ; Blust, Ronny ; De Coen, Wim M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-eb772c6ecf464533c704f38dc56d1b0df5028e6d4ae2b5959212e5ee71d4f8403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>acid-base balance</topic><topic>animal cuticle</topic><topic>animal growth</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Applied ecology</topic><topic>aquatic arthropods</topic><topic>Biological and medical sciences</topic><topic>Cadmium</topic><topic>Cadmium - toxicity</topic><topic>Cadmium Poisoning - genetics</topic><topic>Cadmium Poisoning - metabolism</topic><topic>Daphnia - drug effects</topic><topic>Daphnia - physiology</topic><topic>Daphnia magna</topic><topic>digestion</topic><topic>dose response</topic><topic>Dose-Response Relationship, Drug</topic><topic>ecotoxicogenomics</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>embryogenesis</topic><topic>energy balance</topic><topic>Energy budget</topic><topic>Energy Metabolism</topic><topic>esterases</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Gene Expression Profiling</topic><topic>General aspects</topic><topic>immune response</topic><topic>metabolism</topic><topic>Microarray</topic><topic>microarray technology</topic><topic>Multiple levels of biological organization</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>physiological response</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>signal transduction</topic><topic>stress tolerance</topic><topic>toxicity</topic><topic>Toxicogenomics</topic><topic>vision</topic><topic>vitellogenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Soetaert, Anneleen</creatorcontrib><creatorcontrib>Vandenbrouck, Tine</creatorcontrib><creatorcontrib>van der Ven, Karlijn</creatorcontrib><creatorcontrib>Maras, Marleen</creatorcontrib><creatorcontrib>van Remortel, Piet</creatorcontrib><creatorcontrib>Blust, Ronny</creatorcontrib><creatorcontrib>De Coen, Wim M.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Aqualine</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Aquatic toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soetaert, Anneleen</au><au>Vandenbrouck, Tine</au><au>van der Ven, Karlijn</au><au>Maras, Marleen</au><au>van Remortel, Piet</au><au>Blust, Ronny</au><au>De Coen, Wim M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular responses during cadmium-induced stress in Daphnia magna: Integration of differential gene expression with higher-level effects</atitle><jtitle>Aquatic toxicology</jtitle><addtitle>Aquat Toxicol</addtitle><date>2007-07-20</date><risdate>2007</risdate><volume>83</volume><issue>3</issue><spage>212</spage><epage>222</epage><pages>212-222</pages><issn>0166-445X</issn><eissn>1879-1514</eissn><coden>AQTODG</coden><abstract>DNA microarrays offer great potential in revealing insight into mechanistic toxicity of contaminants. The aim of the present study was (i) to gain insight in concentration- and time-dependent cadmium-induced molecular responses by using a customized
Daphnia magna microarray, and (ii) to compare the gene expression profiles with effects at higher levels of biological organization (e.g. total energy budget and growth). Daphnids were exposed to three cadmium concentrations (nominal value of 10, 50, 100
μg/l) for two time intervals (48 and 96
h). In general, dynamic expression patterns were obtained with a clear increase of gene expression changes at higher concentrations and longer exposure duration. Microarray analysis revealed cadmium affected molecular pathways associated with processes such as digestion, oxygen transport, cuticula metabolism and embryo development. These effects were compared with higher-level effects (energy budgets and growth). For instance, next to reduced energy budgets due to a decline in lipid, carbohydrate and protein content, we found an up-regulated expression of genes related to digestive processes (e.g. α-esterase, cellulase, α-amylase). Furthermore, cadmium affected the expression of genes coding for proteins involved in molecular pathways associated with immune response, stress response, cell adhesion, visual perception and signal transduction in the present study.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17582521</pmid><doi>10.1016/j.aquatox.2007.04.010</doi><tpages>11</tpages></addata></record> |
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| subjects | acid-base balance animal cuticle animal growth Animal, plant and microbial ecology Animals Applied ecology aquatic arthropods Biological and medical sciences Cadmium Cadmium - toxicity Cadmium Poisoning - genetics Cadmium Poisoning - metabolism Daphnia - drug effects Daphnia - physiology Daphnia magna digestion dose response Dose-Response Relationship, Drug ecotoxicogenomics Ecotoxicology, biological effects of pollution embryogenesis energy balance Energy budget Energy Metabolism esterases Freshwater Fundamental and applied biological sciences. Psychology gene expression Gene Expression Profiling General aspects immune response metabolism Microarray microarray technology Multiple levels of biological organization Oligonucleotide Array Sequence Analysis physiological response Reverse Transcriptase Polymerase Chain Reaction signal transduction stress tolerance toxicity Toxicogenomics vision vitellogenin |
| title | Molecular responses during cadmium-induced stress in Daphnia magna: Integration of differential gene expression with higher-level effects |
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