Quantification of homogentisate-1,2-dioxygenase expression in a fungus degrading ethylbenzene

A quantitative real time reverse transcription polymerase chain reaction (qRT-PCR) assay was utilized to quantify the expression of ElHDO in the fungus Exophiala lecanii-corni during the biodegradation of ethylbenzene and other volatile organic pollutants. The assay was applied to measure the impact...

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Veröffentlicht in:	Journal of microbiological methods 2006-11, Vol.67 (2), p.257-265
Hauptverfasser:	Gunsch, Claudia K., Kinney, Kerry A., Szaniszlo, Paul J., Whitman, Christian P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Benzene Derivatives - metabolism Biodegradation, Environmental Biological and medical sciences Bioremediation Comparative quantification Exophiala Exophiala - enzymology Exophiala - genetics Fundamental and applied biological sciences. Psychology Gene expression Homogentisate 1,2-Dioxygenase - blood Homogentisate 1,2-Dioxygenase - genetics Homogentisate 1,2-Dioxygenase - metabolism Microbiology Mycological methods and techniques used in mycology Mycology Pentanones - metabolism Phenylacetates - metabolism Quantitative RT-PCR Real time PCR Reverse Transcriptase Polymerase Chain Reaction Water Pollutants, Chemical - metabolism Xylenes - metabolism
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