A new isolate of Melolontha melolontha entomopoxvirus in Turkey: Morphology, infectivity and prevalence in the field
A virus infection caused by Melolontha melolontha L. (Coleoptera: Scarabaeidae) entomopoxvirus (MmEPV) was recorded in 0.87% of 1,260 larvae collected in 2001–2002 in Trabzon, Turkey. The diseased larvae had dark brown or black and white spots, compared to the normal white-cream colour of healthy la...
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| Veröffentlicht in: | Applied Entomology and Zoology 2006, Vol.41(3), pp.471-477 |
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| creator | Sezen, K.(Karadeniz Technical Univ., Trabzon (Turkey)) Demirbag, Z |
| description | A virus infection caused by Melolontha melolontha L. (Coleoptera: Scarabaeidae) entomopoxvirus (MmEPV) was recorded in 0.87% of 1,260 larvae collected in 2001–2002 in Trabzon, Turkey. The diseased larvae had dark brown or black and white spots, compared to the normal white-cream colour of healthy larvae. MmEPV infections reduced the larval life span from 6 mo to 15±5 d (Mean±S.D.). Based on light and electron microscopic observations, the average length of the spheroids was 5.45±2.34 μm (Mean±S.D.) and the average width was 2.89±1.13 μm (Mean±S.D.) (n=50). The presence of a virus was also demonstrated in a DNA-DNA hybridization assay. The bioassays carried out to determine the infectivity of MmEPV proved its effectiveness against the third-instar larvae of the M. melolontha. The highest insecticidal activity determined in the M. melolontha larvae was 93.3% at the concentration of 7.5×106 spheroids/ml. Our results suggest that a Turkish isolate of MmEPV is promising for biological control of the M. melolontha larvae. |
| doi_str_mv | 10.1303/aez.2006.471 |
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(Coleoptera: Scarabaeidae) entomopoxvirus (MmEPV) was recorded in 0.87% of 1,260 larvae collected in 2001–2002 in Trabzon, Turkey. The diseased larvae had dark brown or black and white spots, compared to the normal white-cream colour of healthy larvae. MmEPV infections reduced the larval life span from 6 mo to 15±5 d (Mean±S.D.). Based on light and electron microscopic observations, the average length of the spheroids was 5.45±2.34 μm (Mean±S.D.) and the average width was 2.89±1.13 μm (Mean±S.D.) (n=50). The presence of a virus was also demonstrated in a DNA-DNA hybridization assay. The bioassays carried out to determine the infectivity of MmEPV proved its effectiveness against the third-instar larvae of the M. melolontha. The highest insecticidal activity determined in the M. melolontha larvae was 93.3% at the concentration of 7.5×106 spheroids/ml. 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(Coleoptera: Scarabaeidae) entomopoxvirus (MmEPV) was recorded in 0.87% of 1,260 larvae collected in 2001–2002 in Trabzon, Turkey. The diseased larvae had dark brown or black and white spots, compared to the normal white-cream colour of healthy larvae. MmEPV infections reduced the larval life span from 6 mo to 15±5 d (Mean±S.D.). Based on light and electron microscopic observations, the average length of the spheroids was 5.45±2.34 μm (Mean±S.D.) and the average width was 2.89±1.13 μm (Mean±S.D.) (n=50). The presence of a virus was also demonstrated in a DNA-DNA hybridization assay. The bioassays carried out to determine the infectivity of MmEPV proved its effectiveness against the third-instar larvae of the M. melolontha. The highest insecticidal activity determined in the M. melolontha larvae was 93.3% at the concentration of 7.5×106 spheroids/ml. Our results suggest that a Turkish isolate of MmEPV is promising for biological control of the M. melolontha larvae.</description><subject>AGENT DE LUTTE BIOLOGIQUE</subject><subject>AGENTES DE CONTROL BIOLOGICO</subject><subject>biological control</subject><subject>BIOLOGICAL CONTROL AGENTS</subject><subject>Coleoptera</subject><subject>Entomopoxvirus</subject><subject>INFECCION</subject><subject>INFECTION</subject><subject>insect virus</subject><subject>INSECTA</subject><subject>Melolontha</subject><subject>Melolontha melolontha</subject><subject>Melolontha melolontha entomopoxvirus</subject><subject>PIEGE</subject><subject>PLANTE</subject><subject>PLANTS</subject><subject>POXVIRIDAE</subject><subject>Scarabaeidae</subject><subject>TRAMPAS</subject><subject>TRAPS</subject><issn>0003-6862</issn><issn>1347-605X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNpFkc1vEzEQxS0EEqFw44rkE6du8NfaXi6oqsqXWpVDkbhZjjNOXJz1Yjsp6V-P01Tl8mY085t3eIPQW0rmlBP-wcL9nBEi50LRZ2hGuVCdJP2v52hGCOGd1JK9RK9KuSWEiUGLGapneIQ7HEqKtgJOHl9BTDGNdW3x5n8LY02bNKW_u5C3BYcR32zzb9h_xFcpT-uGrfanbezB1bALdY_tuMRThp2NMDo4XNQ1YB8gLl-jF97GAm8e6wn6-fni5vxrd3n95dv52WXneiVqR3uhejU4aKKZdcwrRb2TdlhIrZeSC7JgjGulgGkley-BkwUZeiXFkqsFP0Hvj75TTn-2UKrZhOIgRjtC2hZDB90LTnQDT4-gy6mUDN5MOWxs3htKzCFa06I1h2hNi7bhn474bal2BU-wzTW4CA-woIY_iqJPG7e22cDYHN4dHbxNxq5yKOb7j-av2mM0YfwfC3eNLg</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>Sezen, K.(Karadeniz Technical Univ., Trabzon (Turkey))</creator><creator>Demirbag, Z</creator><general>JAPANESE SOCIETY OF APPLIED ENTOMOLOGY AND ZOOLOGY</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>2006</creationdate><title>A new isolate of Melolontha melolontha entomopoxvirus in Turkey: Morphology, infectivity and prevalence in the field</title><author>Sezen, K.(Karadeniz Technical Univ., Trabzon (Turkey)) ; Demirbag, Z</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c574t-1547579ce57982ac2f771fc6a9b688d6340b223877e28765f6e30b095764d37b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>AGENT DE LUTTE BIOLOGIQUE</topic><topic>AGENTES DE CONTROL BIOLOGICO</topic><topic>biological control</topic><topic>BIOLOGICAL CONTROL AGENTS</topic><topic>Coleoptera</topic><topic>Entomopoxvirus</topic><topic>INFECCION</topic><topic>INFECTION</topic><topic>insect virus</topic><topic>INSECTA</topic><topic>Melolontha</topic><topic>Melolontha melolontha</topic><topic>Melolontha melolontha entomopoxvirus</topic><topic>PIEGE</topic><topic>PLANTE</topic><topic>PLANTS</topic><topic>POXVIRIDAE</topic><topic>Scarabaeidae</topic><topic>TRAMPAS</topic><topic>TRAPS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sezen, K.(Karadeniz Technical Univ., Trabzon (Turkey))</creatorcontrib><creatorcontrib>Demirbag, Z</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Applied Entomology and Zoology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sezen, K.(Karadeniz Technical Univ., Trabzon (Turkey))</au><au>Demirbag, Z</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new isolate of Melolontha melolontha entomopoxvirus in Turkey: Morphology, infectivity and prevalence in the field</atitle><jtitle>Applied Entomology and Zoology</jtitle><date>2006</date><risdate>2006</risdate><volume>41</volume><issue>3</issue><spage>471</spage><epage>477</epage><pages>471-477</pages><issn>0003-6862</issn><eissn>1347-605X</eissn><abstract>A virus infection caused by Melolontha melolontha L. (Coleoptera: Scarabaeidae) entomopoxvirus (MmEPV) was recorded in 0.87% of 1,260 larvae collected in 2001–2002 in Trabzon, Turkey. The diseased larvae had dark brown or black and white spots, compared to the normal white-cream colour of healthy larvae. MmEPV infections reduced the larval life span from 6 mo to 15±5 d (Mean±S.D.). Based on light and electron microscopic observations, the average length of the spheroids was 5.45±2.34 μm (Mean±S.D.) and the average width was 2.89±1.13 μm (Mean±S.D.) (n=50). The presence of a virus was also demonstrated in a DNA-DNA hybridization assay. The bioassays carried out to determine the infectivity of MmEPV proved its effectiveness against the third-instar larvae of the M. melolontha. The highest insecticidal activity determined in the M. melolontha larvae was 93.3% at the concentration of 7.5×106 spheroids/ml. Our results suggest that a Turkish isolate of MmEPV is promising for biological control of the M. melolontha larvae.</abstract><pub>JAPANESE SOCIETY OF APPLIED ENTOMOLOGY AND ZOOLOGY</pub><doi>10.1303/aez.2006.471</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied Entomology and Zoology, 2006, Vol.41(3), pp.471-477 |
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| source | J-STAGE Free; EZB-FREE-00999 freely available EZB journals |
| subjects | AGENT DE LUTTE BIOLOGIQUE AGENTES DE CONTROL BIOLOGICO biological control BIOLOGICAL CONTROL AGENTS Coleoptera Entomopoxvirus INFECCION INFECTION insect virus INSECTA Melolontha Melolontha melolontha Melolontha melolontha entomopoxvirus PIEGE PLANTE PLANTS POXVIRIDAE Scarabaeidae TRAMPAS TRAPS |
| title | A new isolate of Melolontha melolontha entomopoxvirus in Turkey: Morphology, infectivity and prevalence in the field |
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