Production of baculoviruses and expression of green fluorescent protein in immobilised Sf21 insect cell cultivation
Spodoptera frugiperda (Sf21) insect cells were grown in the microspheres that were prepared using sodium-cellulosesulfate (NaCS) and poly-diallyldimethylammoniumchloride (PDADMAC). The highest Sf21 cell density was 1 × 10 8 cells/ml in the microshperes. The immobilised Sf21 cells were infected with...
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| Veröffentlicht in: | Biochemical engineering journal 2006-04, Vol.29 (1), p.55-61 |
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| creator | Son, Jeong Hwa Buchholz, Rainer Park, Jung-Keug Kim, Sung Koo |
| description | Spodoptera frugiperda (Sf21) insect cells were grown in the microspheres that were prepared using sodium-cellulosesulfate (NaCS) and poly-diallyldimethylammoniumchloride (PDADMAC). The highest Sf21 cell density was 1
×
10
8
cells/ml in the microshperes. The immobilised Sf21 cells were infected with
Autograpa californica multiple nuclear polyhedrosis virus (AcMNPV) at a “theoretical” MOI of 1.0 and a TOI of 3.9
×
10
7
cells/ml in the microspheres. The AcMNPV polyhedral inclusion bodies (PIBs) titer reached was 1.76
×
10
10
PIBs/ml in the microspheres, which was 58 times higher than that (2.75
×
10
7
PIBs/ml) in a suspension condition. The immobilised Sf21 cells were infected with a recombinant baculovirus, Ac-omega-GFP baculovirus, and the highest concentration of green fluorescent protein (GFP) produced was 0.0048
mg/ml in suspension culture and 0.159
mg/ml in the microspheres. |
| doi_str_mv | 10.1016/j.bej.2005.02.029 |
| format | Article |
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×
10
8
cells/ml in the microshperes. The immobilised Sf21 cells were infected with
Autograpa californica multiple nuclear polyhedrosis virus (AcMNPV) at a “theoretical” MOI of 1.0 and a TOI of 3.9
×
10
7
cells/ml in the microspheres. The AcMNPV polyhedral inclusion bodies (PIBs) titer reached was 1.76
×
10
10
PIBs/ml in the microspheres, which was 58 times higher than that (2.75
×
10
7
PIBs/ml) in a suspension condition. The immobilised Sf21 cells were infected with a recombinant baculovirus, Ac-omega-GFP baculovirus, and the highest concentration of green fluorescent protein (GFP) produced was 0.0048
mg/ml in suspension culture and 0.159
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×
10
8
cells/ml in the microshperes. The immobilised Sf21 cells were infected with
Autograpa californica multiple nuclear polyhedrosis virus (AcMNPV) at a “theoretical” MOI of 1.0 and a TOI of 3.9
×
10
7
cells/ml in the microspheres. The AcMNPV polyhedral inclusion bodies (PIBs) titer reached was 1.76
×
10
10
PIBs/ml in the microspheres, which was 58 times higher than that (2.75
×
10
7
PIBs/ml) in a suspension condition. The immobilised Sf21 cells were infected with a recombinant baculovirus, Ac-omega-GFP baculovirus, and the highest concentration of green fluorescent protein (GFP) produced was 0.0048
mg/ml in suspension culture and 0.159
mg/ml in the microspheres.</description><subject>Ac-omega-GFP baculovirus</subject><subject>Baculovirus</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GFP</subject><subject>Immobilisation</subject><subject>NaCS</subject><subject>Nuclear polyhedrosis virus</subject><subject>PDADMAC</subject><subject>PIBs</subject><subject>Spodoptera frugiperda</subject><issn>1369-703X</issn><issn>1873-295X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNp9kEFr3DAQhU1oIWmaH5CbLsnN25G1smRyKiFpC4EWmsDehCyNghavtdXYS_PvI7MLvRUeSIy-eTN6VXXNYcWBt1-2qx63qwZArqAp6s6qC66VqJtObj6Uu2i7WoHYnFefiLYA0AqlLir6lZOf3RTTyFJgvXXzkA4xz4TE7OgZ_t1nJDq9v2bEkYVhTqXocJzYPqcJ48gW7Xapj0Mk9Ox3aHipEbqJORwGVnyneLDLoM_Vx2AHwqvTeVm9PD4833-vn35--3H_9al2Quqp7uQahBQQwHO1dlwqb3sJmre-k14J0SgpeAs6SOmwb0XfhYJr0YBsnViLy-r26Ft2_DMjTWYXaVnGjphmMrzTaw16AfkRdDkRZQxmn-PO5jfDwSzxmq0p8ZolXgNNUVd6bk7mlpwdQraji_SvUbWy0RoKd3fksPz0EDEbchFHhz7mEo7xKf5nyjutyJCR</recordid><startdate>20060401</startdate><enddate>20060401</enddate><creator>Son, Jeong Hwa</creator><creator>Buchholz, Rainer</creator><creator>Park, Jung-Keug</creator><creator>Kim, Sung Koo</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SS</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20060401</creationdate><title>Production of baculoviruses and expression of green fluorescent protein in immobilised Sf21 insect cell cultivation</title><author>Son, Jeong Hwa ; Buchholz, Rainer ; Park, Jung-Keug ; Kim, Sung Koo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-95403530f0d174c157dab50816d95d73327531608f55ceb63b9f530832056c343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Ac-omega-GFP baculovirus</topic><topic>Baculovirus</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GFP</topic><topic>Immobilisation</topic><topic>NaCS</topic><topic>Nuclear polyhedrosis virus</topic><topic>PDADMAC</topic><topic>PIBs</topic><topic>Spodoptera frugiperda</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Son, Jeong Hwa</creatorcontrib><creatorcontrib>Buchholz, Rainer</creatorcontrib><creatorcontrib>Park, Jung-Keug</creatorcontrib><creatorcontrib>Kim, Sung Koo</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biochemical engineering journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Son, Jeong Hwa</au><au>Buchholz, Rainer</au><au>Park, Jung-Keug</au><au>Kim, Sung Koo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of baculoviruses and expression of green fluorescent protein in immobilised Sf21 insect cell cultivation</atitle><jtitle>Biochemical engineering journal</jtitle><date>2006-04-01</date><risdate>2006</risdate><volume>29</volume><issue>1</issue><spage>55</spage><epage>61</epage><pages>55-61</pages><issn>1369-703X</issn><eissn>1873-295X</eissn><abstract>Spodoptera frugiperda (Sf21) insect cells were grown in the microspheres that were prepared using sodium-cellulosesulfate (NaCS) and poly-diallyldimethylammoniumchloride (PDADMAC). The highest Sf21 cell density was 1
×
10
8
cells/ml in the microshperes. The immobilised Sf21 cells were infected with
Autograpa californica multiple nuclear polyhedrosis virus (AcMNPV) at a “theoretical” MOI of 1.0 and a TOI of 3.9
×
10
7
cells/ml in the microspheres. The AcMNPV polyhedral inclusion bodies (PIBs) titer reached was 1.76
×
10
10
PIBs/ml in the microspheres, which was 58 times higher than that (2.75
×
10
7
PIBs/ml) in a suspension condition. The immobilised Sf21 cells were infected with a recombinant baculovirus, Ac-omega-GFP baculovirus, and the highest concentration of green fluorescent protein (GFP) produced was 0.0048
mg/ml in suspension culture and 0.159
mg/ml in the microspheres.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><doi>10.1016/j.bej.2005.02.029</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1369-703X |
| ispartof | Biochemical engineering journal, 2006-04, Vol.29 (1), p.55-61 |
| issn | 1369-703X 1873-295X |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Ac-omega-GFP baculovirus Baculovirus Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology GFP Immobilisation NaCS Nuclear polyhedrosis virus PDADMAC PIBs Spodoptera frugiperda |
| title | Production of baculoviruses and expression of green fluorescent protein in immobilised Sf21 insect cell cultivation |
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