GENE EXPRESSION PROFILE IN HUMAN CELLS EXPOSED TO ZINC
Although Zn is an essential trace metal for humans, a comprehensive view of its effects on cellular functions has not been obtained. We used a DNA microarray to assess transcriptional alterations in human HeLa cells after exposure to a moderate concentration of Zn (100 μM ZnSO4). Out of 9,182 human...
Gespeichert in:
| Veröffentlicht in: | Journal of toxicological sciences 2007, Vol.32(2), pp.193-196 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 196 |
|---|---|
| container_issue | 2 |
| container_start_page | 193 |
| container_title | Journal of toxicological sciences |
| container_volume | 32 |
| creator | YAMADA, Hirotomo SUZUKI, Kaoru KOIZUMI, Shinji |
| description | Although Zn is an essential trace metal for humans, a comprehensive view of its effects on cellular functions has not been obtained. We used a DNA microarray to assess transcriptional alterations in human HeLa cells after exposure to a moderate concentration of Zn (100 μM ZnSO4). Out of 9,182 human genes, expression was increased in 7 genes and decreased in 4 genes twofold or greater. Four of the 7 upregulated genes were those coding for metallothionein isoforms or related proteins. An unexpectedly small extent of changes in gene expression might reflect rapid sequestration of Zn ions by metallothioneins, and the absence of most of the other protective responses indicated the non-toxic nature of Zn at this concentration. Comparison with our previous DNA microarray results for 5 μM CdSO4-exposed HeLa cells revealed several genes that are regulated by both metals in parallel, and a gene reciprocally regulated by them. |
| doi_str_mv | 10.2131/jts.32.193 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19846330</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>19846330</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4983-1dcad43cd23df344af19750fb71c3ce4bc332f62730a8ff735a5b7cca944d6b73</originalsourceid><addsrcrecordid>eNpd0E1LwzAcx_EgipvTiy9ACoIHoTPJP23Tk4zZbYXajj2AeAlpmupK92DTHnz3dmxO8JIc8uFL-CF0S3CfEiBPRW36QPvEhzPUJZxjG3zun6MuBs5tAg7uoCtjCoyphx12iTrEc4BTBl3kjoM4sIK36SyYz8MktqazZBRGgRXG1mT5OoitYRBF871I5sGLtUis9zAeXqOLXJZG3xzvHlqOgsVwYkfJOBwOIlsxn4NNMiUzBiqjkOXAmMyJ7zk4Tz2iQGmWKgCau9QDLHmee-BIJ_WUkj5jmZt60EMPh-6u2n412tRivTJKl6Xc6G1jBPE5cwFwC-__wWLbVJv2b4Iwl3MO3OWtejwoVW2NqXQudtVqLatvQbDYbynaLQXQtgstvjsmm3Stsz96HK8FzwdQmFp-6BOQVb1Spf5t0cPRJk8v6lNWQm_gBzZJf5k</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1468883868</pqid></control><display><type>article</type><title>GENE EXPRESSION PROFILE IN HUMAN CELLS EXPOSED TO ZINC</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese</source><source>Free Full-Text Journals in Chemistry</source><creator>YAMADA, Hirotomo ; SUZUKI, Kaoru ; KOIZUMI, Shinji</creator><creatorcontrib>YAMADA, Hirotomo ; SUZUKI, Kaoru ; KOIZUMI, Shinji</creatorcontrib><description>Although Zn is an essential trace metal for humans, a comprehensive view of its effects on cellular functions has not been obtained. We used a DNA microarray to assess transcriptional alterations in human HeLa cells after exposure to a moderate concentration of Zn (100 μM ZnSO4). Out of 9,182 human genes, expression was increased in 7 genes and decreased in 4 genes twofold or greater. Four of the 7 upregulated genes were those coding for metallothionein isoforms or related proteins. An unexpectedly small extent of changes in gene expression might reflect rapid sequestration of Zn ions by metallothioneins, and the absence of most of the other protective responses indicated the non-toxic nature of Zn at this concentration. Comparison with our previous DNA microarray results for 5 μM CdSO4-exposed HeLa cells revealed several genes that are regulated by both metals in parallel, and a gene reciprocally regulated by them.</description><identifier>ISSN: 0388-1350</identifier><identifier>EISSN: 1880-3989</identifier><identifier>DOI: 10.2131/jts.32.193</identifier><identifier>PMID: 17538243</identifier><language>eng</language><publisher>Japan: The Japanese Society of Toxicology</publisher><subject>Gene Expression - drug effects ; Gene Expression Profiling - methods ; HeLa Cells - drug effects ; HeLa Cells - metabolism ; Humans ; Metallothionein - genetics ; Metallothionein - metabolism ; Oligonucleotide Array Sequence Analysis ; RNA, Messenger - metabolism ; Zinc Sulfate - metabolism ; Zinc Sulfate - toxicity</subject><ispartof>The Journal of Toxicological Sciences, 2007, Vol.32(2), pp.193-196</ispartof><rights>2007 The Japanese Society of Toxicology</rights><rights>Copyright Japan Science and Technology Agency 2007</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4983-1dcad43cd23df344af19750fb71c3ce4bc332f62730a8ff735a5b7cca944d6b73</citedby><cites>FETCH-LOGICAL-c4983-1dcad43cd23df344af19750fb71c3ce4bc332f62730a8ff735a5b7cca944d6b73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17538243$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YAMADA, Hirotomo</creatorcontrib><creatorcontrib>SUZUKI, Kaoru</creatorcontrib><creatorcontrib>KOIZUMI, Shinji</creatorcontrib><title>GENE EXPRESSION PROFILE IN HUMAN CELLS EXPOSED TO ZINC</title><title>Journal of toxicological sciences</title><addtitle>J Toxicol Sci</addtitle><description>Although Zn is an essential trace metal for humans, a comprehensive view of its effects on cellular functions has not been obtained. We used a DNA microarray to assess transcriptional alterations in human HeLa cells after exposure to a moderate concentration of Zn (100 μM ZnSO4). Out of 9,182 human genes, expression was increased in 7 genes and decreased in 4 genes twofold or greater. Four of the 7 upregulated genes were those coding for metallothionein isoforms or related proteins. An unexpectedly small extent of changes in gene expression might reflect rapid sequestration of Zn ions by metallothioneins, and the absence of most of the other protective responses indicated the non-toxic nature of Zn at this concentration. Comparison with our previous DNA microarray results for 5 μM CdSO4-exposed HeLa cells revealed several genes that are regulated by both metals in parallel, and a gene reciprocally regulated by them.</description><subject>Gene Expression - drug effects</subject><subject>Gene Expression Profiling - methods</subject><subject>HeLa Cells - drug effects</subject><subject>HeLa Cells - metabolism</subject><subject>Humans</subject><subject>Metallothionein - genetics</subject><subject>Metallothionein - metabolism</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>RNA, Messenger - metabolism</subject><subject>Zinc Sulfate - metabolism</subject><subject>Zinc Sulfate - toxicity</subject><issn>0388-1350</issn><issn>1880-3989</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0E1LwzAcx_EgipvTiy9ACoIHoTPJP23Tk4zZbYXajj2AeAlpmupK92DTHnz3dmxO8JIc8uFL-CF0S3CfEiBPRW36QPvEhzPUJZxjG3zun6MuBs5tAg7uoCtjCoyphx12iTrEc4BTBl3kjoM4sIK36SyYz8MktqazZBRGgRXG1mT5OoitYRBF871I5sGLtUis9zAeXqOLXJZG3xzvHlqOgsVwYkfJOBwOIlsxn4NNMiUzBiqjkOXAmMyJ7zk4Tz2iQGmWKgCau9QDLHmee-BIJ_WUkj5jmZt60EMPh-6u2n412tRivTJKl6Xc6G1jBPE5cwFwC-__wWLbVJv2b4Iwl3MO3OWtejwoVW2NqXQudtVqLatvQbDYbynaLQXQtgstvjsmm3Stsz96HK8FzwdQmFp-6BOQVb1Spf5t0cPRJk8v6lNWQm_gBzZJf5k</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>YAMADA, Hirotomo</creator><creator>SUZUKI, Kaoru</creator><creator>KOIZUMI, Shinji</creator><general>The Japanese Society of Toxicology</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7U7</scope><scope>C1K</scope><scope>SOI</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>2007</creationdate><title>GENE EXPRESSION PROFILE IN HUMAN CELLS EXPOSED TO ZINC</title><author>YAMADA, Hirotomo ; SUZUKI, Kaoru ; KOIZUMI, Shinji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4983-1dcad43cd23df344af19750fb71c3ce4bc332f62730a8ff735a5b7cca944d6b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Gene Expression - drug effects</topic><topic>Gene Expression Profiling - methods</topic><topic>HeLa Cells - drug effects</topic><topic>HeLa Cells - metabolism</topic><topic>Humans</topic><topic>Metallothionein - genetics</topic><topic>Metallothionein - metabolism</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>RNA, Messenger - metabolism</topic><topic>Zinc Sulfate - metabolism</topic><topic>Zinc Sulfate - toxicity</topic><toplevel>online_resources</toplevel><creatorcontrib>YAMADA, Hirotomo</creatorcontrib><creatorcontrib>SUZUKI, Kaoru</creatorcontrib><creatorcontrib>KOIZUMI, Shinji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of toxicological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YAMADA, Hirotomo</au><au>SUZUKI, Kaoru</au><au>KOIZUMI, Shinji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GENE EXPRESSION PROFILE IN HUMAN CELLS EXPOSED TO ZINC</atitle><jtitle>Journal of toxicological sciences</jtitle><addtitle>J Toxicol Sci</addtitle><date>2007</date><risdate>2007</risdate><volume>32</volume><issue>2</issue><spage>193</spage><epage>196</epage><pages>193-196</pages><issn>0388-1350</issn><eissn>1880-3989</eissn><abstract>Although Zn is an essential trace metal for humans, a comprehensive view of its effects on cellular functions has not been obtained. We used a DNA microarray to assess transcriptional alterations in human HeLa cells after exposure to a moderate concentration of Zn (100 μM ZnSO4). Out of 9,182 human genes, expression was increased in 7 genes and decreased in 4 genes twofold or greater. Four of the 7 upregulated genes were those coding for metallothionein isoforms or related proteins. An unexpectedly small extent of changes in gene expression might reflect rapid sequestration of Zn ions by metallothioneins, and the absence of most of the other protective responses indicated the non-toxic nature of Zn at this concentration. Comparison with our previous DNA microarray results for 5 μM CdSO4-exposed HeLa cells revealed several genes that are regulated by both metals in parallel, and a gene reciprocally regulated by them.</abstract><cop>Japan</cop><pub>The Japanese Society of Toxicology</pub><pmid>17538243</pmid><doi>10.2131/jts.32.193</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0388-1350 |
| ispartof | The Journal of Toxicological Sciences, 2007, Vol.32(2), pp.193-196 |
| issn | 0388-1350 1880-3989 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19846330 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Free Full-Text Journals in Chemistry |
| subjects | Gene Expression - drug effects Gene Expression Profiling - methods HeLa Cells - drug effects HeLa Cells - metabolism Humans Metallothionein - genetics Metallothionein - metabolism Oligonucleotide Array Sequence Analysis RNA, Messenger - metabolism Zinc Sulfate - metabolism Zinc Sulfate - toxicity |
| title | GENE EXPRESSION PROFILE IN HUMAN CELLS EXPOSED TO ZINC |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T17%3A57%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=GENE%20EXPRESSION%20PROFILE%20IN%20HUMAN%20CELLS%20EXPOSED%20TO%20ZINC&rft.jtitle=Journal%20of%20toxicological%20sciences&rft.au=YAMADA,%20Hirotomo&rft.date=2007&rft.volume=32&rft.issue=2&rft.spage=193&rft.epage=196&rft.pages=193-196&rft.issn=0388-1350&rft.eissn=1880-3989&rft_id=info:doi/10.2131/jts.32.193&rft_dat=%3Cproquest_cross%3E19846330%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1468883868&rft_id=info:pmid/17538243&rfr_iscdi=true |