AN IMPROVIZED TECHNIQUE FOR MEASUREMENT OF NITROGEN FIXATION BY BLUE GREEN ALGAE AND AZOLLA USING MOIST SOIL CORES FROM RICE FIELDS

A modified technique for measurement of nitrogen fixation by blue green algae and Azolla sp. using intact, moist soil cores was developed for use in field studies in which chemical fertilizers, blue green algae and Azolla biofertilizers had been used in association with a rice crop. This method invo...

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Veröffentlicht in:Experimental agriculture 2003-04, Vol.39 (2), p.145-150
Hauptverfasser: PRASANNA, RADHA, TRIPATHI, U., DOMINIC, T. K., SINGH, A. K., YADAV, A. K., SINGH, P. K.
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container_issue 2
container_start_page 145
container_title Experimental agriculture
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creator PRASANNA, RADHA
TRIPATHI, U.
DOMINIC, T. K.
SINGH, A. K.
YADAV, A. K.
SINGH, P. K.
description A modified technique for measurement of nitrogen fixation by blue green algae and Azolla sp. using intact, moist soil cores was developed for use in field studies in which chemical fertilizers, blue green algae and Azolla biofertilizers had been used in association with a rice crop. This method involves collection of fresh and moist soil cores (0–30 mm) using a soil auger, incubation with 10% acetylene in airtight glass vials under field conditions for three hours, and measurement of the ethylene produced using a gas chromatograph. Experiments carried out over a period of three consecutive years revealed that the estimates of nitrogen fixation were comparable with data of other researchers obtained through the use of soil water columns, sophisticated chambers and automated sampling devices. This simplified technique, therefore, can provide rapid and reliable measurements of nitrogen fixation in field experiments.
doi_str_mv 10.1017/S0014479702001187
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This method involves collection of fresh and moist soil cores (0–30 mm) using a soil auger, incubation with 10% acetylene in airtight glass vials under field conditions for three hours, and measurement of the ethylene produced using a gas chromatograph. Experiments carried out over a period of three consecutive years revealed that the estimates of nitrogen fixation were comparable with data of other researchers obtained through the use of soil water columns, sophisticated chambers and automated sampling devices. This simplified technique, therefore, can provide rapid and reliable measurements of nitrogen fixation in field experiments.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><doi>10.1017/S0014479702001187</doi><tpages>6</tpages></addata></record>
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ispartof Experimental agriculture, 2003-04, Vol.39 (2), p.145-150
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source Cambridge Journals
subjects Agrochemicals
Agronomy. Soil science and plant productions
Algae
Aquatic plants
Azolla
Biofertilizers
Biological and medical sciences
Cereal crops
Cores
Cyanobacteria
Cyanophyta
Field tests
Fundamental and applied biological sciences. Psychology
General agronomy. Plant production
Moisture content
Nitrogen
Nitrogen fertilization
Nitrogen fixation
Nitrogen, phosphorus, potassium fertilizations
Oryza sativa
Rice
Rice fields
Soil water
Soil-plant relationships. Soil fertility. Fertilization. Amendments
Statistical analysis
title AN IMPROVIZED TECHNIQUE FOR MEASUREMENT OF NITROGEN FIXATION BY BLUE GREEN ALGAE AND AZOLLA USING MOIST SOIL CORES FROM RICE FIELDS
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