Effects of telmisartan and losartan on irradiated testes
To analyze the effects of radiation on the reproductive tissue of male Wistar rats and to evaluate whether treatment with the Ang II AT1 receptor antagonists telmisartan and losartan mitigate the dysfunctions resulting from this exposure. Rats were randomly divided into groups: Control, Irradiated,...
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| Veröffentlicht in: | Life sciences (1973) 2018-02, Vol.194, p.157-167 |
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| creator | da Silva Mansano, Naira Jorge, Isabela Fernandes Chies, Agnaldo Bruno Viani, Gustavo Arruda Spadella, Maria Angélica |
| description | To analyze the effects of radiation on the reproductive tissue of male Wistar rats and to evaluate whether treatment with the Ang II AT1 receptor antagonists telmisartan and losartan mitigate the dysfunctions resulting from this exposure.
Rats were randomly divided into groups: Control, Irradiated, Telmisartan, Losartan, Irradiated+Telmisartan, and Irradiated+Losartan. Single dose of 5Gy was administered directly into the scrotum, followed by treatment with telmisartan (12mg/kg/day) or losartan (34mg/kg/two times/day) for 60days. Testicular function parameters were evaluated from spermatozoa of the vas deferens. Testes were processed for histopathological and morphometric-stereological analysis. Proliferating cell nuclear antigen (PCNA) immunohistochemistry was evaluated.
Radiation significantly reduced sperm motility, concentration, vitality, and increased the number of abnormal spermatozoa. Telmisartan and losartan did not significantly prevent these radiation-induced disorders. Seminiferous tubules were atrophied in both untreated and treated irradiated testes, and exhibited vacuoles, increased interstitial tissue and high number of blood vessels. However, several seminiferous tubules in recuperation were founded among damaged tubules in the testes of treated animals. The PCNA immunohistochemistry confirmed these outcomes. PCNA-positive cells were detected in dividing spermatogonia and spermatocytes from irradiated telmisartan and losartan treated rats whereas in the only-irradiated group, PCNA staining was observed in the nuclei of only the surviving spermatogonia.
Under these experimental conditions, the testicular function parameters showed that radiation produced marked damage that was not reversed by treatments. However, gonadal restructuring and recovery of spermatogenesis in treated animals may to reflect attenuation of radiation-induced damages and potential start of recovery. |
| doi_str_mv | 10.1016/j.lfs.2017.12.031 |
| format | Article |
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Rats were randomly divided into groups: Control, Irradiated, Telmisartan, Losartan, Irradiated+Telmisartan, and Irradiated+Losartan. Single dose of 5Gy was administered directly into the scrotum, followed by treatment with telmisartan (12mg/kg/day) or losartan (34mg/kg/two times/day) for 60days. Testicular function parameters were evaluated from spermatozoa of the vas deferens. Testes were processed for histopathological and morphometric-stereological analysis. Proliferating cell nuclear antigen (PCNA) immunohistochemistry was evaluated.
Radiation significantly reduced sperm motility, concentration, vitality, and increased the number of abnormal spermatozoa. Telmisartan and losartan did not significantly prevent these radiation-induced disorders. Seminiferous tubules were atrophied in both untreated and treated irradiated testes, and exhibited vacuoles, increased interstitial tissue and high number of blood vessels. However, several seminiferous tubules in recuperation were founded among damaged tubules in the testes of treated animals. The PCNA immunohistochemistry confirmed these outcomes. PCNA-positive cells were detected in dividing spermatogonia and spermatocytes from irradiated telmisartan and losartan treated rats whereas in the only-irradiated group, PCNA staining was observed in the nuclei of only the surviving spermatogonia.
Under these experimental conditions, the testicular function parameters showed that radiation produced marked damage that was not reversed by treatments. However, gonadal restructuring and recovery of spermatogenesis in treated animals may to reflect attenuation of radiation-induced damages and potential start of recovery.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2017.12.031</identifier><identifier>PMID: 29287783</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Angiotensin II ; Angiotensin II Type 1 Receptor Blockers - pharmacology ; Animals ; Benzimidazoles - pharmacology ; Benzoates - pharmacology ; Blood vessels ; Cells ; Impact analysis ; Ionizing radiation ; Losartan ; Losartan - pharmacology ; Male ; Nuclei ; Oxidative stress ; Proliferating cell nuclear antigen ; Radiation ; Radiation effects ; Radiation-Protective Agents - pharmacology ; Radiotherapy ; Rat testes ; Rats ; Rats, Wistar ; Scrotum ; Sperm ; Spermatocytes ; Spermatogenesis - drug effects ; Spermatogenesis - radiation effects ; Spermatogonia ; Spermatozoa ; Spermatozoa - drug effects ; Spermatozoa - pathology ; Spermatozoa - radiation effects ; Studies ; Telmisartan ; Testes ; Testis - drug effects ; Testis - pathology ; Testis - physiopathology ; Testis - radiation effects ; Tubules ; Vacuoles ; Vas deferens</subject><ispartof>Life sciences (1973), 2018-02, Vol.194, p.157-167</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><rights>Copyright Elsevier BV Feb 1, 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-4333d57ee25600e780c6ccff882110351a55908d0334892f4a6fbaff9521be243</citedby><cites>FETCH-LOGICAL-c490t-4333d57ee25600e780c6ccff882110351a55908d0334892f4a6fbaff9521be243</cites><orcidid>0000-0003-4545-6434</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.lfs.2017.12.031$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29287783$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Silva Mansano, Naira</creatorcontrib><creatorcontrib>Jorge, Isabela Fernandes</creatorcontrib><creatorcontrib>Chies, Agnaldo Bruno</creatorcontrib><creatorcontrib>Viani, Gustavo Arruda</creatorcontrib><creatorcontrib>Spadella, Maria Angélica</creatorcontrib><title>Effects of telmisartan and losartan on irradiated testes</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>To analyze the effects of radiation on the reproductive tissue of male Wistar rats and to evaluate whether treatment with the Ang II AT1 receptor antagonists telmisartan and losartan mitigate the dysfunctions resulting from this exposure.
Rats were randomly divided into groups: Control, Irradiated, Telmisartan, Losartan, Irradiated+Telmisartan, and Irradiated+Losartan. Single dose of 5Gy was administered directly into the scrotum, followed by treatment with telmisartan (12mg/kg/day) or losartan (34mg/kg/two times/day) for 60days. Testicular function parameters were evaluated from spermatozoa of the vas deferens. Testes were processed for histopathological and morphometric-stereological analysis. Proliferating cell nuclear antigen (PCNA) immunohistochemistry was evaluated.
Radiation significantly reduced sperm motility, concentration, vitality, and increased the number of abnormal spermatozoa. Telmisartan and losartan did not significantly prevent these radiation-induced disorders. Seminiferous tubules were atrophied in both untreated and treated irradiated testes, and exhibited vacuoles, increased interstitial tissue and high number of blood vessels. However, several seminiferous tubules in recuperation were founded among damaged tubules in the testes of treated animals. The PCNA immunohistochemistry confirmed these outcomes. PCNA-positive cells were detected in dividing spermatogonia and spermatocytes from irradiated telmisartan and losartan treated rats whereas in the only-irradiated group, PCNA staining was observed in the nuclei of only the surviving spermatogonia.
Under these experimental conditions, the testicular function parameters showed that radiation produced marked damage that was not reversed by treatments. However, gonadal restructuring and recovery of spermatogenesis in treated animals may to reflect attenuation of radiation-induced damages and potential start of recovery.</description><subject>Angiotensin II</subject><subject>Angiotensin II Type 1 Receptor Blockers - pharmacology</subject><subject>Animals</subject><subject>Benzimidazoles - pharmacology</subject><subject>Benzoates - pharmacology</subject><subject>Blood vessels</subject><subject>Cells</subject><subject>Impact analysis</subject><subject>Ionizing radiation</subject><subject>Losartan</subject><subject>Losartan - pharmacology</subject><subject>Male</subject><subject>Nuclei</subject><subject>Oxidative stress</subject><subject>Proliferating cell nuclear antigen</subject><subject>Radiation</subject><subject>Radiation effects</subject><subject>Radiation-Protective Agents - pharmacology</subject><subject>Radiotherapy</subject><subject>Rat testes</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Scrotum</subject><subject>Sperm</subject><subject>Spermatocytes</subject><subject>Spermatogenesis - drug effects</subject><subject>Spermatogenesis - radiation effects</subject><subject>Spermatogonia</subject><subject>Spermatozoa</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - pathology</subject><subject>Spermatozoa - radiation effects</subject><subject>Studies</subject><subject>Telmisartan</subject><subject>Testes</subject><subject>Testis - drug effects</subject><subject>Testis - pathology</subject><subject>Testis - physiopathology</subject><subject>Testis - radiation effects</subject><subject>Tubules</subject><subject>Vacuoles</subject><subject>Vas deferens</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1Lw0AQhhdRtFZ_gBcJePGSOLMfyQZPUuoHFLzoednuBySkSd1NBP-9W1o9eBAGhoFn3hkeQq4QCgQs79qi87GggFWBtACGR2SGsqpzKBkekxkA5TmjIM7IeYwtAAhRsVNyRmsqq0qyGZFL750ZYzb4bHTdpok6jLrPdG-zbjgMQ581IWjb6NHZhMVUF-TE6y66y0Ofk_fH5dviOV-9Pr0sHla54TWMOWeMWVE5R0UJ4CoJpjTGeykpIjCBWogapAXGuKyp57r0a-19LSiuHeVsTm73udswfEzptEo_Gtd1unfDFBXWkkpOS14l9OYP2g5T6NN3iiYTQJEhSxTuKROGGIPzahuajQ5fCkHttKpWJa1qp1UhVUlr2rk-JE_rjbO_Gz8eE3C_B1xS8dm4oKJpXG-cbULSq-zQ_BP_DQObhYw</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>da Silva Mansano, Naira</creator><creator>Jorge, Isabela Fernandes</creator><creator>Chies, Agnaldo Bruno</creator><creator>Viani, Gustavo Arruda</creator><creator>Spadella, Maria Angélica</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4545-6434</orcidid></search><sort><creationdate>20180201</creationdate><title>Effects of telmisartan and losartan on irradiated testes</title><author>da Silva Mansano, Naira ; Jorge, Isabela Fernandes ; Chies, Agnaldo Bruno ; Viani, Gustavo Arruda ; Spadella, Maria Angélica</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-4333d57ee25600e780c6ccff882110351a55908d0334892f4a6fbaff9521be243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Angiotensin II</topic><topic>Angiotensin II Type 1 Receptor Blockers - pharmacology</topic><topic>Animals</topic><topic>Benzimidazoles - pharmacology</topic><topic>Benzoates - pharmacology</topic><topic>Blood vessels</topic><topic>Cells</topic><topic>Impact analysis</topic><topic>Ionizing radiation</topic><topic>Losartan</topic><topic>Losartan - pharmacology</topic><topic>Male</topic><topic>Nuclei</topic><topic>Oxidative stress</topic><topic>Proliferating cell nuclear antigen</topic><topic>Radiation</topic><topic>Radiation effects</topic><topic>Radiation-Protective Agents - pharmacology</topic><topic>Radiotherapy</topic><topic>Rat testes</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Scrotum</topic><topic>Sperm</topic><topic>Spermatocytes</topic><topic>Spermatogenesis - drug effects</topic><topic>Spermatogenesis - radiation effects</topic><topic>Spermatogonia</topic><topic>Spermatozoa</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - pathology</topic><topic>Spermatozoa - radiation effects</topic><topic>Studies</topic><topic>Telmisartan</topic><topic>Testes</topic><topic>Testis - drug effects</topic><topic>Testis - pathology</topic><topic>Testis - physiopathology</topic><topic>Testis - radiation effects</topic><topic>Tubules</topic><topic>Vacuoles</topic><topic>Vas deferens</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>da Silva Mansano, Naira</creatorcontrib><creatorcontrib>Jorge, Isabela Fernandes</creatorcontrib><creatorcontrib>Chies, Agnaldo Bruno</creatorcontrib><creatorcontrib>Viani, Gustavo Arruda</creatorcontrib><creatorcontrib>Spadella, Maria Angélica</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>da Silva Mansano, Naira</au><au>Jorge, Isabela Fernandes</au><au>Chies, Agnaldo Bruno</au><au>Viani, Gustavo Arruda</au><au>Spadella, Maria Angélica</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of telmisartan and losartan on irradiated testes</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2018-02-01</date><risdate>2018</risdate><volume>194</volume><spage>157</spage><epage>167</epage><pages>157-167</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>To analyze the effects of radiation on the reproductive tissue of male Wistar rats and to evaluate whether treatment with the Ang II AT1 receptor antagonists telmisartan and losartan mitigate the dysfunctions resulting from this exposure.
Rats were randomly divided into groups: Control, Irradiated, Telmisartan, Losartan, Irradiated+Telmisartan, and Irradiated+Losartan. Single dose of 5Gy was administered directly into the scrotum, followed by treatment with telmisartan (12mg/kg/day) or losartan (34mg/kg/two times/day) for 60days. Testicular function parameters were evaluated from spermatozoa of the vas deferens. Testes were processed for histopathological and morphometric-stereological analysis. Proliferating cell nuclear antigen (PCNA) immunohistochemistry was evaluated.
Radiation significantly reduced sperm motility, concentration, vitality, and increased the number of abnormal spermatozoa. Telmisartan and losartan did not significantly prevent these radiation-induced disorders. Seminiferous tubules were atrophied in both untreated and treated irradiated testes, and exhibited vacuoles, increased interstitial tissue and high number of blood vessels. However, several seminiferous tubules in recuperation were founded among damaged tubules in the testes of treated animals. The PCNA immunohistochemistry confirmed these outcomes. PCNA-positive cells were detected in dividing spermatogonia and spermatocytes from irradiated telmisartan and losartan treated rats whereas in the only-irradiated group, PCNA staining was observed in the nuclei of only the surviving spermatogonia.
Under these experimental conditions, the testicular function parameters showed that radiation produced marked damage that was not reversed by treatments. However, gonadal restructuring and recovery of spermatogenesis in treated animals may to reflect attenuation of radiation-induced damages and potential start of recovery.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>29287783</pmid><doi>10.1016/j.lfs.2017.12.031</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-4545-6434</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Angiotensin II Angiotensin II Type 1 Receptor Blockers - pharmacology Animals Benzimidazoles - pharmacology Benzoates - pharmacology Blood vessels Cells Impact analysis Ionizing radiation Losartan Losartan - pharmacology Male Nuclei Oxidative stress Proliferating cell nuclear antigen Radiation Radiation effects Radiation-Protective Agents - pharmacology Radiotherapy Rat testes Rats Rats, Wistar Scrotum Sperm Spermatocytes Spermatogenesis - drug effects Spermatogenesis - radiation effects Spermatogonia Spermatozoa Spermatozoa - drug effects Spermatozoa - pathology Spermatozoa - radiation effects Studies Telmisartan Testes Testis - drug effects Testis - pathology Testis - physiopathology Testis - radiation effects Tubules Vacuoles Vas deferens |
| title | Effects of telmisartan and losartan on irradiated testes |
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