Enhancement of Cry19Aa mosquitocidal activity against Aedes aegypti by mutations in the putative loop regions of domain II

Improvements in the mosquitocidal activity of Bacillus thuringiensis Cry19Aa were achieved by protein engineering of putative surface loop residues in domain II through rational design. The improvement of Aedes toxicity in Cry19Aa was 42,000-fold and did not affect its toxicity against Anopheles or...

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Veröffentlicht in:	Applied and Environmental Microbiology 2004-06, Vol.70 (6), p.3769-3771
Hauptverfasser:	Abdullah, M.A.F, Dean, D.H
Format:	Artikel
Sprache:	eng
Schlagworte:	Aedes - drug effects Aedes aegypti Amino Acid Sequence amino acid sequences Animals Anopheles Anopheles quadrimaculatus Bacillus thuringiensis Bacillus thuringiensis - metabolism bacterial insecticides Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - toxicity bacterial toxins Bacterial Toxins - chemistry Bacterial Toxins - genetics Bacterial Toxins - toxicity Biochemistry Biopesticides crystal proteins Culex Culex pipiens Culex quinquefasciatus Culicidae Endotoxins - chemistry Endotoxins - genetics Endotoxins - toxicity Genetic engineering Hemolysin Proteins insecticidal properties Invertebrate Microbiology Models, Molecular Molecular Sequence Data Mosquitoes mutants Mutation Pest Control, Biological protein engineering Protein Engineering - methods sequence alignment
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Beschreibung
Zusammenfassung:	Improvements in the mosquitocidal activity of Bacillus thuringiensis Cry19Aa were achieved by protein engineering of putative surface loop residues in domain II through rational design. The improvement of Aedes toxicity in Cry19Aa was 42,000-fold and did not affect its toxicity against Anopheles or Culex.
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.70.6.3769-3771.2004