Metabolic compensation of steroidal glycoalkaloid biosynthesis in transgenic potato tubers: using reverse genetics to confirm the in vivo enzyme function of a steroidal alkaloid galactosyltransferase
Steroidal glycoalkaloids (SGAs) are secondary metabolites of Solanaceous plants. Two predominant glycoalkaloids, α-chaconine and α-solanine are produced in potatoes. An antisense transgene was constructed to down-regulate glycoalkaloid biosynthesis using a potato cDNA encoding a sterol alkaloid glyc...
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| Veröffentlicht in: | Plant science (Limerick) 2005, Vol.168 (1), p.267-273 |
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| creator | McCue, Kent F. Shepherd, Louise V.T. Allen, Paul V. Maccree, M. Malendia Rockhold, David R. Corsini, Dennis L. Davies, Howard V. Belknap, William R. |
| description | Steroidal glycoalkaloids (SGAs) are secondary metabolites of Solanaceous plants. Two predominant glycoalkaloids, α-chaconine and α-solanine are produced in potatoes. An antisense transgene was constructed to down-regulate glycoalkaloid biosynthesis using a potato cDNA encoding a sterol alkaloid glycosyltransferase (
Sgt1). Introduction of this construct into potatoes resulted in some lines with an almost complete inhibition of α-solanine accumulation. This inhibition was compensated by elevated levels of α-chaconine and resulted in wild type total SGA levels in the transgenic lines. In vitro assays with the recombinant SGT1 isolated from yeast demonstrated that
Sgt1 encodes an enzyme capable of both glucosyltransferase and galactosyltransferase activity with a preference for UDP-galactose as the sugar donor. Together this data confirms SGT1's role in vivo as the solanidine:UDP-galactose galactosyltransferase. |
| doi_str_mv | 10.1016/j.plantsci.2004.08.006 |
| format | Article |
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Sgt1). Introduction of this construct into potatoes resulted in some lines with an almost complete inhibition of α-solanine accumulation. This inhibition was compensated by elevated levels of α-chaconine and resulted in wild type total SGA levels in the transgenic lines. In vitro assays with the recombinant SGT1 isolated from yeast demonstrated that
Sgt1 encodes an enzyme capable of both glucosyltransferase and galactosyltransferase activity with a preference for UDP-galactose as the sugar donor. Together this data confirms SGT1's role in vivo as the solanidine:UDP-galactose galactosyltransferase.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/j.plantsci.2004.08.006</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Antisense ; Biological and medical sciences ; biosynthesis ; Chaconine ; chemical structure ; enzyme activity ; Fundamental and applied biological sciences. Psychology ; Galactosyl transferase ; galactosyltransferases ; Genetic engineering applications ; Genetics and breeding of economic plants ; glycoalkaloids ; messenger RNA ; Plant breeding: fundamental aspects and methodology ; potatoes ; recombinant fusion proteins ; reverse genetics ; Secondary metabolism ; Solanine ; Solanum tuberosum ; steroids ; Transgene ; transgenes ; transgenic plants ; tubers</subject><ispartof>Plant science (Limerick), 2005, Vol.168 (1), p.267-273</ispartof><rights>2004 Elsevier Ireland Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-5b17ba517cbc66ccd97728ddbead20948620259f3c9734ba06d5f7a75139818b3</citedby><cites>FETCH-LOGICAL-c461t-5b17ba517cbc66ccd97728ddbead20948620259f3c9734ba06d5f7a75139818b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168945204003760$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16295290$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>McCue, Kent F.</creatorcontrib><creatorcontrib>Shepherd, Louise V.T.</creatorcontrib><creatorcontrib>Allen, Paul V.</creatorcontrib><creatorcontrib>Maccree, M. Malendia</creatorcontrib><creatorcontrib>Rockhold, David R.</creatorcontrib><creatorcontrib>Corsini, Dennis L.</creatorcontrib><creatorcontrib>Davies, Howard V.</creatorcontrib><creatorcontrib>Belknap, William R.</creatorcontrib><title>Metabolic compensation of steroidal glycoalkaloid biosynthesis in transgenic potato tubers: using reverse genetics to confirm the in vivo enzyme function of a steroidal alkaloid galactosyltransferase</title><title>Plant science (Limerick)</title><description>Steroidal glycoalkaloids (SGAs) are secondary metabolites of Solanaceous plants. Two predominant glycoalkaloids, α-chaconine and α-solanine are produced in potatoes. An antisense transgene was constructed to down-regulate glycoalkaloid biosynthesis using a potato cDNA encoding a sterol alkaloid glycosyltransferase (
Sgt1). Introduction of this construct into potatoes resulted in some lines with an almost complete inhibition of α-solanine accumulation. This inhibition was compensated by elevated levels of α-chaconine and resulted in wild type total SGA levels in the transgenic lines. In vitro assays with the recombinant SGT1 isolated from yeast demonstrated that
Sgt1 encodes an enzyme capable of both glucosyltransferase and galactosyltransferase activity with a preference for UDP-galactose as the sugar donor. Together this data confirms SGT1's role in vivo as the solanidine:UDP-galactose galactosyltransferase.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Antisense</subject><subject>Biological and medical sciences</subject><subject>biosynthesis</subject><subject>Chaconine</subject><subject>chemical structure</subject><subject>enzyme activity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galactosyl transferase</subject><subject>galactosyltransferases</subject><subject>Genetic engineering applications</subject><subject>Genetics and breeding of economic plants</subject><subject>glycoalkaloids</subject><subject>messenger RNA</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>potatoes</subject><subject>recombinant fusion proteins</subject><subject>reverse genetics</subject><subject>Secondary metabolism</subject><subject>Solanine</subject><subject>Solanum tuberosum</subject><subject>steroids</subject><subject>Transgene</subject><subject>transgenes</subject><subject>transgenic plants</subject><subject>tubers</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkU-P0zAQxSMEEmXhK4AvcGuxncRJOIFWyx9pEQfYszVxxsXFsYPHrVS-IF8Ll-4CN07WSL9573leVT0VfCO4UC93m8VDyGTcRnLebHi_4Vzdq1ai7-q1lO1wv1oVsF8PTSsfVo-Idpxz2bbdqvr5ETOM0TvDTJwXDATZxcCiZZQxRTeBZ1t_NBH8N_BlZqOLdAz5K5Ij5gLLCQJtMRSJJWbIkeX9iIlesT25sGUJD2VCVhDMzhArhInBujSzonKSOLhDZBh-HGdkdh_MXQT4J8Qf_y14MLlk8L-dLSYgfFw9sOAJn9y-F9XN26svl-_X15_efbh8c702jRJ53Y6iG6EVnRmNUsZMQ9fJfppGhEnyoemVLIcZbG2Grm5G4GpqbQddK-qhF_1YX1QvzrpLit_3SFnPjgz60gDGPWlRMFVuXkB1Bk2KRAmtXpKbIR214PrUm97pu970qTfNe116K4vPbx2ADHhb_mgc_d1WcmjlwAv37MxZiBq2qTA3nyUXNeeDaur-RLw-E1gOcnCYdPHCYHByCU3WU3T_C_MLbX3CYg</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>McCue, Kent F.</creator><creator>Shepherd, Louise V.T.</creator><creator>Allen, Paul V.</creator><creator>Maccree, M. Malendia</creator><creator>Rockhold, David R.</creator><creator>Corsini, Dennis L.</creator><creator>Davies, Howard V.</creator><creator>Belknap, William R.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>2005</creationdate><title>Metabolic compensation of steroidal glycoalkaloid biosynthesis in transgenic potato tubers: using reverse genetics to confirm the in vivo enzyme function of a steroidal alkaloid galactosyltransferase</title><author>McCue, Kent F. ; Shepherd, Louise V.T. ; Allen, Paul V. ; Maccree, M. 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Sgt1). Introduction of this construct into potatoes resulted in some lines with an almost complete inhibition of α-solanine accumulation. This inhibition was compensated by elevated levels of α-chaconine and resulted in wild type total SGA levels in the transgenic lines. In vitro assays with the recombinant SGT1 isolated from yeast demonstrated that
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| identifier | ISSN: 0168-9452 |
| ispartof | Plant science (Limerick), 2005, Vol.168 (1), p.267-273 |
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| source | Elsevier ScienceDirect Journals |
| subjects | Agronomy. Soil science and plant productions Antisense Biological and medical sciences biosynthesis Chaconine chemical structure enzyme activity Fundamental and applied biological sciences. Psychology Galactosyl transferase galactosyltransferases Genetic engineering applications Genetics and breeding of economic plants glycoalkaloids messenger RNA Plant breeding: fundamental aspects and methodology potatoes recombinant fusion proteins reverse genetics Secondary metabolism Solanine Solanum tuberosum steroids Transgene transgenes transgenic plants tubers |
| title | Metabolic compensation of steroidal glycoalkaloid biosynthesis in transgenic potato tubers: using reverse genetics to confirm the in vivo enzyme function of a steroidal alkaloid galactosyltransferase |
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