Determination of estrogenic activity in landfill leachate by simplified yeast two-hybrid assay

We previously developed a simplified yeast two-hybrid assay of estrogenic activity. In the present study, the optimal conditions for sensitivity and stability of this assay were decided. The assay could determine the estrogenic activity of 4 x 10(-11) mol dm(-1) 17beta-estradiol and also anti-estrog...

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Veröffentlicht in:	Journal of environmental monitoring 2002-12, Vol.4 (6), p.1040-1046
Hauptverfasser:	Kawagoshi, Yasunori, Tsukagoshi, Yukiko, Fukunaga, Isao
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological Assay - methods Cell Division Environmental Monitoring - methods Estradiol - pharmacology Estrogen Antagonists - pharmacology estrogens Hydrocarbons - adverse effects Organisms, Genetically Modified Phenols - adverse effects Receptors, Estrogen - drug effects Reference Values Refuse Disposal Soil Pollutants - adverse effects Tamoxifen - analogs & derivatives Tamoxifen - pharmacology Water Pollutants - adverse effects Yeasts - genetics Yeasts - physiology
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