Characterization and functional assay of a fatty acyl‐CoA reductase gene in the scale insect, Ericerus pela Chavannes (Hemiptera: Coccoidae)
Ericerus pela Chavannes (Hemiptera: Coccoidae) is an economically important scale insect because the second instar males secrete a harvestable wax‐like substance. In this study, we report the molecular cloning of a fatty acyl‐CoA reductase gene (EpFAR) of E. pela. We predicted a 520‐aa protein with...
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| Veröffentlicht in: | Archives of insect biochemistry and physiology 2018-04, Vol.97 (4), p.n/a |
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| creator | Hu, Yan‐Hong Chen, Xiao‐Ming Yang, Pu Ding, Wei‐Feng |
| description | Ericerus pela Chavannes (Hemiptera: Coccoidae) is an economically important scale insect because the second instar males secrete a harvestable wax‐like substance. In this study, we report the molecular cloning of a fatty acyl‐CoA reductase gene (EpFAR) of E. pela. We predicted a 520‐aa protein with the FAR family features from the deduced amino acid sequence. The EpFAR mRNA was expressed in five tested tissues, testis, alimentary canal, fat body, Malpighian tubules, and mostly in cuticle. The EpFAR protein was localized by immunofluorescence only in the wax glands and testis. EpFAR expression in High Five insect cells documented the recombinant EpFAR reduced 26‐0:(S) CoA and to its corresponding alcohol. The data illuminate the molecular mechanism for fatty alcohol biosynthesis in a beneficial insect, E. pela. |
| doi_str_mv | 10.1002/arch.21445 |
| format | Article |
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In this study, we report the molecular cloning of a fatty acyl‐CoA reductase gene (EpFAR) of E. pela. We predicted a 520‐aa protein with the FAR family features from the deduced amino acid sequence. The EpFAR mRNA was expressed in five tested tissues, testis, alimentary canal, fat body, Malpighian tubules, and mostly in cuticle. The EpFAR protein was localized by immunofluorescence only in the wax glands and testis. EpFAR expression in High Five insect cells documented the recombinant EpFAR reduced 26‐0:(S) CoA and to its corresponding alcohol. The data illuminate the molecular mechanism for fatty alcohol biosynthesis in a beneficial insect, E. pela.</description><identifier>ISSN: 0739-4462</identifier><identifier>EISSN: 1520-6327</identifier><identifier>DOI: 10.1002/arch.21445</identifier><identifier>PMID: 29277917</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Alcohol ; Alcohols ; Amino acid sequence ; Biosynthesis ; Cloning ; distribution characterization ; Ericerus pela ; Fat body ; fatty acyl‐CoA reductase ; fatty alcohol ; Gastrointestinal tract ; Glands ; Hemiptera ; Immunofluorescence ; Insect cells ; Insects ; Males ; Malpighian tubules ; mRNA ; Reductase ; Tissues</subject><ispartof>Archives of insect biochemistry and physiology, 2018-04, Vol.97 (4), p.n/a</ispartof><rights>2017 Wiley Periodicals, Inc.</rights><rights>Copyright © 2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3575-d753251f4385ebead3de6a98311a5f55ac6e759418adfba90d3b4f06751b1033</citedby><cites>FETCH-LOGICAL-c3575-d753251f4385ebead3de6a98311a5f55ac6e759418adfba90d3b4f06751b1033</cites><orcidid>0000-0003-3989-9925</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Farch.21445$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Farch.21445$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29277917$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Yan‐Hong</creatorcontrib><creatorcontrib>Chen, Xiao‐Ming</creatorcontrib><creatorcontrib>Yang, Pu</creatorcontrib><creatorcontrib>Ding, Wei‐Feng</creatorcontrib><title>Characterization and functional assay of a fatty acyl‐CoA reductase gene in the scale insect, Ericerus pela Chavannes (Hemiptera: Coccoidae)</title><title>Archives of insect biochemistry and physiology</title><addtitle>Arch Insect Biochem Physiol</addtitle><description>Ericerus pela Chavannes (Hemiptera: Coccoidae) is an economically important scale insect because the second instar males secrete a harvestable wax‐like substance. 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The data illuminate the molecular mechanism for fatty alcohol biosynthesis in a beneficial insect, E. pela.</description><subject>Alcohol</subject><subject>Alcohols</subject><subject>Amino acid sequence</subject><subject>Biosynthesis</subject><subject>Cloning</subject><subject>distribution characterization</subject><subject>Ericerus pela</subject><subject>Fat body</subject><subject>fatty acyl‐CoA reductase</subject><subject>fatty alcohol</subject><subject>Gastrointestinal tract</subject><subject>Glands</subject><subject>Hemiptera</subject><subject>Immunofluorescence</subject><subject>Insect cells</subject><subject>Insects</subject><subject>Males</subject><subject>Malpighian tubules</subject><subject>mRNA</subject><subject>Reductase</subject><subject>Tissues</subject><issn>0739-4462</issn><issn>1520-6327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kc2K1EAUhQtRnJ7RjQ8gBW5mxIz1m6TcNWG0hQFBZl_cVN3YNaRTbVWixJVPID6jT2J6enThwtXlwMd34B5CnnF2yRkTryG57aXgSukHZMW1YEUpRfWQrFglTaFUKU7Iac63jDFT8voxORFGVJXh1Yr8aLaQwI2YwjcYQxwoDJ520-AOAXoKOcNMY0eBdjCOMwU397--_2zimib0kxshI_2EA9Iw0HGLNDvoDyGjG1_RqxQcpinTPfZAl7YvMAyY6fkGd2G_9MIb2kTnYvCAF0_Iow76jE_v7xm5eXt102yK6w_v3jfr68JJXenCV1oKzTsla40tgpceSzC15Bx0pzW4EittFK_Bdy0Y5mWrOlZWmrecSXlGzo_afYqfJ8yj3YXssO9hwDhly03NtDTqDn3xD3obp7R8JlvBuFSmNrVaqJdHyqWYc8LO7lPYQZotZ_Ywkj2MZO9GWuDn98qp3aH_i_5ZZQH4Efgaepz_o7Lrj83mKP0N5YGdlg</recordid><startdate>201804</startdate><enddate>201804</enddate><creator>Hu, Yan‐Hong</creator><creator>Chen, Xiao‐Ming</creator><creator>Yang, Pu</creator><creator>Ding, Wei‐Feng</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3989-9925</orcidid></search><sort><creationdate>201804</creationdate><title>Characterization and functional assay of a fatty acyl‐CoA reductase gene in the scale insect, Ericerus pela Chavannes (Hemiptera: Coccoidae)</title><author>Hu, Yan‐Hong ; Chen, Xiao‐Ming ; Yang, Pu ; Ding, Wei‐Feng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3575-d753251f4385ebead3de6a98311a5f55ac6e759418adfba90d3b4f06751b1033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alcohol</topic><topic>Alcohols</topic><topic>Amino acid sequence</topic><topic>Biosynthesis</topic><topic>Cloning</topic><topic>distribution characterization</topic><topic>Ericerus pela</topic><topic>Fat body</topic><topic>fatty acyl‐CoA reductase</topic><topic>fatty alcohol</topic><topic>Gastrointestinal tract</topic><topic>Glands</topic><topic>Hemiptera</topic><topic>Immunofluorescence</topic><topic>Insect cells</topic><topic>Insects</topic><topic>Males</topic><topic>Malpighian tubules</topic><topic>mRNA</topic><topic>Reductase</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Yan‐Hong</creatorcontrib><creatorcontrib>Chen, Xiao‐Ming</creatorcontrib><creatorcontrib>Yang, Pu</creatorcontrib><creatorcontrib>Ding, Wei‐Feng</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of insect biochemistry and physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Yan‐Hong</au><au>Chen, Xiao‐Ming</au><au>Yang, Pu</au><au>Ding, Wei‐Feng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and functional assay of a fatty acyl‐CoA reductase gene in the scale insect, Ericerus pela Chavannes (Hemiptera: Coccoidae)</atitle><jtitle>Archives of insect biochemistry and physiology</jtitle><addtitle>Arch Insect Biochem Physiol</addtitle><date>2018-04</date><risdate>2018</risdate><volume>97</volume><issue>4</issue><epage>n/a</epage><issn>0739-4462</issn><eissn>1520-6327</eissn><abstract>Ericerus pela Chavannes (Hemiptera: Coccoidae) is an economically important scale insect because the second instar males secrete a harvestable wax‐like substance. In this study, we report the molecular cloning of a fatty acyl‐CoA reductase gene (EpFAR) of E. pela. We predicted a 520‐aa protein with the FAR family features from the deduced amino acid sequence. The EpFAR mRNA was expressed in five tested tissues, testis, alimentary canal, fat body, Malpighian tubules, and mostly in cuticle. The EpFAR protein was localized by immunofluorescence only in the wax glands and testis. EpFAR expression in High Five insect cells documented the recombinant EpFAR reduced 26‐0:(S) CoA and to its corresponding alcohol. The data illuminate the molecular mechanism for fatty alcohol biosynthesis in a beneficial insect, E. pela.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29277917</pmid><doi>10.1002/arch.21445</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-3989-9925</orcidid></addata></record> |
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| subjects | Alcohol Alcohols Amino acid sequence Biosynthesis Cloning distribution characterization Ericerus pela Fat body fatty acyl‐CoA reductase fatty alcohol Gastrointestinal tract Glands Hemiptera Immunofluorescence Insect cells Insects Males Malpighian tubules mRNA Reductase Tissues |
| title | Characterization and functional assay of a fatty acyl‐CoA reductase gene in the scale insect, Ericerus pela Chavannes (Hemiptera: Coccoidae) |
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