High-yield expression and purification of soluble forms of the anti- apoptotic Bcl-x sub(L) and Bcl-2 as TolAIII-fusion proteins

A method is presented to produce large amounts of Bcl-2 and Bcl-x sub(L), two anti-apoptotic proteins of considerable biomedical interest. Expression constructs were prepared in which the Escherichia coli protein TolAIII, known to promote over expression of soluble product, was added to the N- termi...

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Veröffentlicht in:	Protein expression and purification 2008-08, Vol.60 (2), p.214-220
Hauptverfasser:	Nedelkina, Svetlana, Gokce, Isa, Ridley, Helen, Weckerle, Celine, Magnin, Thierry, Vallette, Francois, Pattus, Franc, Lakey, Jeremy H, Bechinger, Burkhard
Format:	Artikel
Sprache:	eng
Schlagworte:	Escherichia coli
Online-Zugang:	Volltext
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