A recombinant Escherichia coli expressing an α-alkyl-β-ketoester reductase with unusual stereoselectivity
A recombinant Escherichia coli expressing an α-alkyl-β-ketoester reductase that yields the anti (2R,3R) α-alkyl-β-hydroxyester as the major product was constructed. The enzyme was identified from genomic libraries of Paucimonas lemoignei, cloned and expressed in E. coli providing a whole cell biocat...
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| Veröffentlicht in: | Biocatalysis and biotransformation 2007-01, Vol.25 (5), p.414-417 |
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| container_title | Biocatalysis and biotransformation |
| container_volume | 25 |
| creator | Panizza, Paola Onetto, Silvia Rodríguez, Sonia |
| description | A recombinant Escherichia coli expressing an α-alkyl-β-ketoester reductase that yields the anti (2R,3R) α-alkyl-β-hydroxyester as the major product was constructed. The enzyme was identified from genomic libraries of Paucimonas lemoignei, cloned and expressed in E. coli providing a whole cell biocatalyst with novel stereoselectivity. This constitutes the first report on a whole cell biocatalyst for the synthesis of (2R,3R) α-alkyl-β-hydroxy-esters. |
| doi_str_mv | 10.1080/10242420701510684 |
| format | Article |
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| fulltext | fulltext |
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| ispartof | Biocatalysis and biotransformation, 2007-01, Vol.25 (5), p.414-417 |
| issn | 1024-2422 1029-2446 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19802692 |
| source | Taylor & Francis Journals Complete |
| subjects | Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Escherichia coli Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Paucimonas lemoignei recombinant E. coli reductase β-ketoester |
| title | A recombinant Escherichia coli expressing an α-alkyl-β-ketoester reductase with unusual stereoselectivity |
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