Capillary Isoelectric Focusing-Mass Spectrometry Method for the Separation and Online Characterization of Intact Monoclonal Antibody Charge Variants

We report a new online capillary isoelectric focusing-mass spectrometry (CIEF-MS) method for monoclonal antibody (mAb) charge variant analysis using an electrokinetically pumped sheath-flow nanospray ion source and a time-of-flight MS with pressure-assisted chemical mobilization. To develop a succes...

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Veröffentlicht in:	Analytical chemistry (Washington) 2018-02, Vol.90 (3), p.2246-2254
Hauptverfasser:	Dai, Jun, Lamp, Jared, Xia, Qiangwei, Zhang, Yingru
Format:	Artikel
Sprache:	eng
Schlagworte:	Anolytes Bevacizumab Chemistry Correlation analysis Electrokinetics Electrophoretic mobility Field strength Glycerol Infliximab Internet Ionization Isoelectric focusing Kinetics Markers Mass spectrometry Mass spectroscopy Migration Monoclonal antibodies Optimization Pressure Reagents Spectroscopy Trastuzumab Tumor necrosis factor-α
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