Water soluble two-photon fluorescent organic probes for long-term imaging of lysosomes in live cells and tumor spheroids

The morphological alteration of lysosomes is a powerful indicator of various pathological disorders. In this regard, we have designed and synthesized a new water soluble fluorescent Schiff-base ligand (L-lyso) containing two hydroxyl groups. L-lyso exhibits excellent two-photon properties with track...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2018, Vol.54 (5), p.539-542
Hauptverfasser:	Kumari, Pratibha, Verma, Sanjay K, Mobin, Shaikh M
Format:	Artikel
Sprache:	eng
Schlagworte:	Fluorescence Hydroxyl groups Lysosomes Spheroids Water chemistry
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creator	Kumari, Pratibha Verma, Sanjay K Mobin, Shaikh M
description	The morphological alteration of lysosomes is a powerful indicator of various pathological disorders. In this regard, we have designed and synthesized a new water soluble fluorescent Schiff-base ligand (L-lyso) containing two hydroxyl groups. L-lyso exhibits excellent two-photon properties with tracking of lysosomes in live cells as well as in 3D tumor spheroids. Furthermore, it can label lysosomes for more than 3 days. Thus, L-lyso has an edge over the commercially available expensive LysoTracker probes and also over other reported probes in terms of its long-term imaging, water solubility and facile synthesis.
doi_str_mv	10.1039/c7cc07812a
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subjects	Fluorescence Hydroxyl groups Lysosomes Spheroids Water chemistry
title	Water soluble two-photon fluorescent organic probes for long-term imaging of lysosomes in live cells and tumor spheroids
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