A novel and highly sensitive real-time nested RT-PCR assay in a single closed tube for detection of enterovirus

The sensitivity of qRT-PCR assay is not adequate for the detection of the samples with lower viral load, particularly in the cerebrospinal fluid (CSF) of patients. Here, we present the development of a highly sensitive real-time nested RT-PCR (RTN RT-PCR) assay in a single closed tube for detection...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Diagnostic microbiology and infectious disease 2018-03, Vol.90 (3), p.181-185
Hauptverfasser:	Shen, Xin-xin, Qiu, Fang-zhou, Zhao, Huai-long, Yang, Meng-jie, Hong, Liu, Xu, Song-tao, Zhou, Shuai-feng, Li, Gui-xia, Feng, Zhi-shan, Ma, Xue-jun
Format:	Artikel
Sprache:	eng
Schlagworte:	Detection Human enteroviruses RTN RT-PCR
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!