Knockdown of the Chromatin Remodeling Gene Brahma by RNA Interference Reduces Reproductive Fitness and Lifespan in Common Bed Bug (Hemiptera: Cimicidae)
The common bed bug, Cimex lectularius L. (Hemiptera: Cimicidae) is a nuisance household pest causing significant medical and economic impacts. RNA interference (RNAi) of genes that are involved in vital physiological processes can serve as potential RNAi targets for insect control. Brahma is an ATPa...
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| Veröffentlicht in: | Journal of medical entomology 2018-05, Vol.55 (3), p.534-539 |
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| creator | Basnet, Sanjay Kamble, Shripat T. |
| description | The common bed bug, Cimex lectularius L. (Hemiptera: Cimicidae) is a nuisance household pest causing significant medical and economic impacts. RNA interference (RNAi) of genes that are involved in vital physiological processes can serve as potential RNAi targets for insect control. Brahma is an ATPase subunit of a chromatin-remodeling complex involved in transcription of several genes for cellular processes, most importantly the homeotic genes. In this study, we used a microinjection technique to deliver double stranded RNA into female bed bugs. Delivery of 0.05 and 0.5 μg/insect of brahma dsRNA directly into hemocele resulted substantial reduction in oviposition. Eggs laid by bed bugs receiving both doses of brahma dsRNA exhibited significantly lower hatching percentage as compared to controls. In addition, brahma RNAi in female bed bugs caused significant mortality. Our results disclosed the potential of brahma RNAi to suppress bed bug population through injection of specific dsRNA, suggesting a critical function of this gene in bed bugs' reproduction and survival. Based on our data, brahma can be a promising RNAi target for suppression of bed bug population. |
| doi_str_mv | 10.1093/jme/tjx234 |
| format | Article |
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(Hemiptera: Cimicidae) is a nuisance household pest causing significant medical and economic impacts. RNA interference (RNAi) of genes that are involved in vital physiological processes can serve as potential RNAi targets for insect control. Brahma is an ATPase subunit of a chromatin-remodeling complex involved in transcription of several genes for cellular processes, most importantly the homeotic genes. In this study, we used a microinjection technique to deliver double stranded RNA into female bed bugs. Delivery of 0.05 and 0.5 μg/insect of brahma dsRNA directly into hemocele resulted substantial reduction in oviposition. Eggs laid by bed bugs receiving both doses of brahma dsRNA exhibited significantly lower hatching percentage as compared to controls. In addition, brahma RNAi in female bed bugs caused significant mortality. Our results disclosed the potential of brahma RNAi to suppress bed bug population through injection of specific dsRNA, suggesting a critical function of this gene in bed bugs' reproduction and survival. Based on our data, brahma can be a promising RNAi target for suppression of bed bug population.</description><identifier>ISSN: 0022-2585</identifier><identifier>EISSN: 1938-2928</identifier><identifier>DOI: 10.1093/jme/tjx234</identifier><identifier>PMID: 29272428</identifier><language>eng</language><publisher>England: Entomological Society of America</publisher><subject>Adenosine triphosphatase ; Animals ; Bedbugs - genetics ; Bedbugs - physiology ; Chromatin Assembly and Disassembly - genetics ; Chromatin remodeling ; Cimex lectularius ; Cimicidae ; Double-stranded RNA ; Economic impact ; egg viability ; Eggs ; fecundity ; Females ; Gene expression ; Genes ; Genetic Fitness ; Hatching ; Hemiptera ; Insect control ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Insects ; Interference ; Life span ; Longevity - genetics ; Microinjection ; MOLECULAR BIOLOGY/GENOMICS ; Oviposition ; Reproduction - genetics ; Ribonucleic acid ; RNA ; RNA Interference ; RNA, Double-Stranded - administration & dosage ; RNA-mediated interference ; RNAi ; survival ; Transcription</subject><ispartof>Journal of medical entomology, 2018-05, Vol.55 (3), p.534-539</ispartof><rights>Published by Oxford University Press on behalf of Entomological Society of America 2018. 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(Hemiptera: Cimicidae) is a nuisance household pest causing significant medical and economic impacts. RNA interference (RNAi) of genes that are involved in vital physiological processes can serve as potential RNAi targets for insect control. Brahma is an ATPase subunit of a chromatin-remodeling complex involved in transcription of several genes for cellular processes, most importantly the homeotic genes. In this study, we used a microinjection technique to deliver double stranded RNA into female bed bugs. Delivery of 0.05 and 0.5 μg/insect of brahma dsRNA directly into hemocele resulted substantial reduction in oviposition. Eggs laid by bed bugs receiving both doses of brahma dsRNA exhibited significantly lower hatching percentage as compared to controls. In addition, brahma RNAi in female bed bugs caused significant mortality. Our results disclosed the potential of brahma RNAi to suppress bed bug population through injection of specific dsRNA, suggesting a critical function of this gene in bed bugs' reproduction and survival. Based on our data, brahma can be a promising RNAi target for suppression of bed bug population.</description><subject>Adenosine triphosphatase</subject><subject>Animals</subject><subject>Bedbugs - genetics</subject><subject>Bedbugs - physiology</subject><subject>Chromatin Assembly and Disassembly - genetics</subject><subject>Chromatin remodeling</subject><subject>Cimex lectularius</subject><subject>Cimicidae</subject><subject>Double-stranded RNA</subject><subject>Economic impact</subject><subject>egg viability</subject><subject>Eggs</subject><subject>fecundity</subject><subject>Females</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic Fitness</subject><subject>Hatching</subject><subject>Hemiptera</subject><subject>Insect control</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>Insects</subject><subject>Interference</subject><subject>Life span</subject><subject>Longevity - genetics</subject><subject>Microinjection</subject><subject>MOLECULAR BIOLOGY/GENOMICS</subject><subject>Oviposition</subject><subject>Reproduction - genetics</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>RNA, Double-Stranded - administration & dosage</subject><subject>RNA-mediated interference</subject><subject>RNAi</subject><subject>survival</subject><subject>Transcription</subject><issn>0022-2585</issn><issn>1938-2928</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kd1qFDEYhoNY7Fo98QIk4EktrM3PZCfxrDvYH1wUih4PmeRLN-sm2SYzau_Ey23KVgUPPPoCeXh4X16EXlHyjhLFTzcBTsfNT8abJ2hGFZdzpph8imaEMDZnQopD9LyUDSFE0kY9Q4f1v2UNkzP062NM5ptNPyJODo9rwN06p6BHH_E1hGRh6-MNvoAIeJn1Omg83OHrT2f4Ko6QHWSIBipqJwOl3l1O9Tn674DP_RihFKyjxSvvoOx0xNXbpRBSxEuweDnd4ONLCH5XZfo97nzwxlsNb1-gA6e3BV4-3iP09fzDl-5yvvp8cdWdreYDl81Y27XMEcJFrSbA1cKubZ3SZlAMKEjjqDWqkVaBcNZq0ipJQYBQpDWcW36EjvfeGvx2gjL2wRcD262OkKbSU9UqtWiIWFT0zT_oJk051nQ94wuulGqFqNTJnjI5lZLB9bvsg853PSX9w1593avf71Xh14_KaQhg_6C_B_obb_ApRfif6x7SIp-f</recordid><startdate>20180504</startdate><enddate>20180504</enddate><creator>Basnet, Sanjay</creator><creator>Kamble, Shripat T.</creator><general>Entomological Society of America</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>7X8</scope></search><sort><creationdate>20180504</creationdate><title>Knockdown of the Chromatin Remodeling Gene Brahma by RNA Interference Reduces Reproductive Fitness and Lifespan in Common Bed Bug (Hemiptera: Cimicidae)</title><author>Basnet, Sanjay ; Kamble, Shripat T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b384t-2572f00350815ef022f77f9acb92e1e8cf1dc948d9e5fdda07981e5e5907c33d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adenosine triphosphatase</topic><topic>Animals</topic><topic>Bedbugs - genetics</topic><topic>Bedbugs - physiology</topic><topic>Chromatin Assembly and Disassembly - genetics</topic><topic>Chromatin remodeling</topic><topic>Cimex lectularius</topic><topic>Cimicidae</topic><topic>Double-stranded RNA</topic><topic>Economic impact</topic><topic>egg viability</topic><topic>Eggs</topic><topic>fecundity</topic><topic>Females</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic Fitness</topic><topic>Hatching</topic><topic>Hemiptera</topic><topic>Insect control</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - metabolism</topic><topic>Insects</topic><topic>Interference</topic><topic>Life span</topic><topic>Longevity - genetics</topic><topic>Microinjection</topic><topic>MOLECULAR BIOLOGY/GENOMICS</topic><topic>Oviposition</topic><topic>Reproduction - genetics</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Interference</topic><topic>RNA, Double-Stranded - administration & dosage</topic><topic>RNA-mediated interference</topic><topic>RNAi</topic><topic>survival</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Basnet, Sanjay</creatorcontrib><creatorcontrib>Kamble, Shripat T.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Basnet, Sanjay</au><au>Kamble, Shripat T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Knockdown of the Chromatin Remodeling Gene Brahma by RNA Interference Reduces Reproductive Fitness and Lifespan in Common Bed Bug (Hemiptera: Cimicidae)</atitle><jtitle>Journal of medical entomology</jtitle><addtitle>J Med Entomol</addtitle><date>2018-05-04</date><risdate>2018</risdate><volume>55</volume><issue>3</issue><spage>534</spage><epage>539</epage><pages>534-539</pages><issn>0022-2585</issn><eissn>1938-2928</eissn><abstract>The common bed bug, Cimex lectularius L. (Hemiptera: Cimicidae) is a nuisance household pest causing significant medical and economic impacts. RNA interference (RNAi) of genes that are involved in vital physiological processes can serve as potential RNAi targets for insect control. Brahma is an ATPase subunit of a chromatin-remodeling complex involved in transcription of several genes for cellular processes, most importantly the homeotic genes. In this study, we used a microinjection technique to deliver double stranded RNA into female bed bugs. Delivery of 0.05 and 0.5 μg/insect of brahma dsRNA directly into hemocele resulted substantial reduction in oviposition. Eggs laid by bed bugs receiving both doses of brahma dsRNA exhibited significantly lower hatching percentage as compared to controls. In addition, brahma RNAi in female bed bugs caused significant mortality. Our results disclosed the potential of brahma RNAi to suppress bed bug population through injection of specific dsRNA, suggesting a critical function of this gene in bed bugs' reproduction and survival. Based on our data, brahma can be a promising RNAi target for suppression of bed bug population.</abstract><cop>England</cop><pub>Entomological Society of America</pub><pmid>29272428</pmid><doi>10.1093/jme/tjx234</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2585 |
| ispartof | Journal of medical entomology, 2018-05, Vol.55 (3), p.534-539 |
| issn | 0022-2585 1938-2928 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Adenosine triphosphatase Animals Bedbugs - genetics Bedbugs - physiology Chromatin Assembly and Disassembly - genetics Chromatin remodeling Cimex lectularius Cimicidae Double-stranded RNA Economic impact egg viability Eggs fecundity Females Gene expression Genes Genetic Fitness Hatching Hemiptera Insect control Insect Proteins - genetics Insect Proteins - metabolism Insects Interference Life span Longevity - genetics Microinjection MOLECULAR BIOLOGY/GENOMICS Oviposition Reproduction - genetics Ribonucleic acid RNA RNA Interference RNA, Double-Stranded - administration & dosage RNA-mediated interference RNAi survival Transcription |
| title | Knockdown of the Chromatin Remodeling Gene Brahma by RNA Interference Reduces Reproductive Fitness and Lifespan in Common Bed Bug (Hemiptera: Cimicidae) |
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