Genotoxic and cytotoxic effects of manganese chloride in cultured human lymphocytes treated in different phases of cell cycle
Manganese (Mn) has a natural occurrence and is necessary during the initial periods of the development. However, in high concentrations, Mn can be related to neurodegenerative disorders. The aim of the present study was to evaluate the mutagenic potential of manganese chloride (MnCl2·4H2O). Comet as...
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| Veröffentlicht in: | Toxicology in vitro 2008-06, Vol.22 (4), p.1032-1037 |
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| creator | Lima, P.D.L. Vasconcellos, M.C. Bahia, M.O. Montenegro, R.C. Pessoa, C.O. Costa-Lotufo, L.V. Moraes, M.O. Burbano, R.R. |
| description | Manganese (Mn) has a natural occurrence and is necessary during the initial periods of the development. However, in high concentrations, Mn can be related to neurodegenerative disorders. The aim of the present study was to evaluate the mutagenic potential of manganese chloride (MnCl2·4H2O). Comet assay and chromosome aberrations analysis were applied to determine the DNA-damaging and clastogenic effects of MnCl2·4H2O. Cultured human lymphocytes were treated with 15, 20 and 25μM manganese chloride during the G1, G1/S, S (pulses of 1 and 6h), and G2 phases of the cell cycle. All tested concentrations were cytotoxic and reduced significantly the mitotic index in G1, G1/S and S (1 and 6h) treatments, while in G2 treatment only the higher concentrations (20 and 25μM) showed cytotoxic effects. Clastogenicity and DNA damage were found only in treatments with the highest concentration (25μM). Chromosome aberrations were found exclusively in the G2 phase of the cell cycle. The absence of polyploidy in mitosis, suggests that manganese does not affect the formation of the mitotic spindle with the concentrations tested. The genotoxicity found in G2 phase and in the comet assay can be related to the short time of treatment in both cases. |
| doi_str_mv | 10.1016/j.tiv.2007.12.011 |
| format | Article |
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However, in high concentrations, Mn can be related to neurodegenerative disorders. The aim of the present study was to evaluate the mutagenic potential of manganese chloride (MnCl2·4H2O). Comet assay and chromosome aberrations analysis were applied to determine the DNA-damaging and clastogenic effects of MnCl2·4H2O. Cultured human lymphocytes were treated with 15, 20 and 25μM manganese chloride during the G1, G1/S, S (pulses of 1 and 6h), and G2 phases of the cell cycle. All tested concentrations were cytotoxic and reduced significantly the mitotic index in G1, G1/S and S (1 and 6h) treatments, while in G2 treatment only the higher concentrations (20 and 25μM) showed cytotoxic effects. Clastogenicity and DNA damage were found only in treatments with the highest concentration (25μM). Chromosome aberrations were found exclusively in the G2 phase of the cell cycle. The absence of polyploidy in mitosis, suggests that manganese does not affect the formation of the mitotic spindle with the concentrations tested. The genotoxicity found in G2 phase and in the comet assay can be related to the short time of treatment in both cases.</description><identifier>ISSN: 0887-2333</identifier><identifier>EISSN: 1879-3177</identifier><identifier>DOI: 10.1016/j.tiv.2007.12.011</identifier><identifier>PMID: 18258413</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Cell Cycle - drug effects ; Cells, Cultured ; Chlorides - administration & dosage ; Chlorides - toxicity ; Chromosome aberration ; Chromosome Aberrations - drug effects ; Comet Assay ; Environmental Pollutants - administration & dosage ; Environmental Pollutants - toxicity ; Humans ; Lymphocytes - drug effects ; Lymphocytes - metabolism ; Manganese chloride ; Manganese Compounds - administration & dosage ; Mitotic Index ; Mutagenicity Tests ; Mutagens - administration & dosage ; Mutagens - toxicity ; Time Factors</subject><ispartof>Toxicology in vitro, 2008-06, Vol.22 (4), p.1032-1037</ispartof><rights>2007 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-15a13edc9d4076d73e098d31907f8cb023a268d04e97259e1791a19a4295c1073</citedby><cites>FETCH-LOGICAL-c382t-15a13edc9d4076d73e098d31907f8cb023a268d04e97259e1791a19a4295c1073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0887233307003669$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18258413$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lima, P.D.L.</creatorcontrib><creatorcontrib>Vasconcellos, M.C.</creatorcontrib><creatorcontrib>Bahia, M.O.</creatorcontrib><creatorcontrib>Montenegro, R.C.</creatorcontrib><creatorcontrib>Pessoa, C.O.</creatorcontrib><creatorcontrib>Costa-Lotufo, L.V.</creatorcontrib><creatorcontrib>Moraes, M.O.</creatorcontrib><creatorcontrib>Burbano, R.R.</creatorcontrib><title>Genotoxic and cytotoxic effects of manganese chloride in cultured human lymphocytes treated in different phases of cell cycle</title><title>Toxicology in vitro</title><addtitle>Toxicol In Vitro</addtitle><description>Manganese (Mn) has a natural occurrence and is necessary during the initial periods of the development. However, in high concentrations, Mn can be related to neurodegenerative disorders. The aim of the present study was to evaluate the mutagenic potential of manganese chloride (MnCl2·4H2O). Comet assay and chromosome aberrations analysis were applied to determine the DNA-damaging and clastogenic effects of MnCl2·4H2O. Cultured human lymphocytes were treated with 15, 20 and 25μM manganese chloride during the G1, G1/S, S (pulses of 1 and 6h), and G2 phases of the cell cycle. All tested concentrations were cytotoxic and reduced significantly the mitotic index in G1, G1/S and S (1 and 6h) treatments, while in G2 treatment only the higher concentrations (20 and 25μM) showed cytotoxic effects. Clastogenicity and DNA damage were found only in treatments with the highest concentration (25μM). Chromosome aberrations were found exclusively in the G2 phase of the cell cycle. The absence of polyploidy in mitosis, suggests that manganese does not affect the formation of the mitotic spindle with the concentrations tested. The genotoxicity found in G2 phase and in the comet assay can be related to the short time of treatment in both cases.</description><subject>Cell Cycle - drug effects</subject><subject>Cells, Cultured</subject><subject>Chlorides - administration & dosage</subject><subject>Chlorides - toxicity</subject><subject>Chromosome aberration</subject><subject>Chromosome Aberrations - drug effects</subject><subject>Comet Assay</subject><subject>Environmental Pollutants - administration & dosage</subject><subject>Environmental Pollutants - toxicity</subject><subject>Humans</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - metabolism</subject><subject>Manganese chloride</subject><subject>Manganese Compounds - administration & dosage</subject><subject>Mitotic Index</subject><subject>Mutagenicity Tests</subject><subject>Mutagens - administration & dosage</subject><subject>Mutagens - toxicity</subject><subject>Time Factors</subject><issn>0887-2333</issn><issn>1879-3177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFvFCEYholpY9fqD_BiOHmbkQ9mBogn09hq0sSLngmFb1w2M8MKTOMe-t9lu5t464kQnvfl-x5C3gNrgcHwadeW8NhyxmQLvGUAr8gGlNSNACkvyIYpJRsuhLgib3LeMcZ6xdlrcgWK96oDsSFPd7jEEv8GR-3iqTuU8w3HEV3JNI50tstvu2BG6rZTTMEjDQt161TWhJ5u1wrQ6TDvt7HmMdOS0Jb6Uikfak_CpdD91mZ87nM4TfUnN-FbcjnaKeO783lNft1-_Xnzrbn_cff95st944TipYHegkDvtO-YHLwUyLTyAjSTo3IPjAvLB-VZh1ryXiNIDRa07bjuHTAprsnHU-8-xT8r5mLmkI9j1LXimg1oqeXQDRWEE-hSzDnhaPYpzDYdDDBzdG52pjo3R-cGuKnOa-bDuXx9mNH_T5wlV-DzCcC64mPAZLILuDj0IVXHxsfwQv0_e5uTiA</recordid><startdate>200806</startdate><enddate>200806</enddate><creator>Lima, P.D.L.</creator><creator>Vasconcellos, M.C.</creator><creator>Bahia, M.O.</creator><creator>Montenegro, R.C.</creator><creator>Pessoa, C.O.</creator><creator>Costa-Lotufo, L.V.</creator><creator>Moraes, M.O.</creator><creator>Burbano, R.R.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200806</creationdate><title>Genotoxic and cytotoxic effects of manganese chloride in cultured human lymphocytes treated in different phases of cell cycle</title><author>Lima, P.D.L. ; Vasconcellos, M.C. ; Bahia, M.O. ; Montenegro, R.C. ; Pessoa, C.O. ; Costa-Lotufo, L.V. ; Moraes, M.O. ; Burbano, R.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-15a13edc9d4076d73e098d31907f8cb023a268d04e97259e1791a19a4295c1073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Cell Cycle - drug effects</topic><topic>Cells, Cultured</topic><topic>Chlorides - administration & dosage</topic><topic>Chlorides - toxicity</topic><topic>Chromosome aberration</topic><topic>Chromosome Aberrations - drug effects</topic><topic>Comet Assay</topic><topic>Environmental Pollutants - administration & dosage</topic><topic>Environmental Pollutants - toxicity</topic><topic>Humans</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - metabolism</topic><topic>Manganese chloride</topic><topic>Manganese Compounds - administration & dosage</topic><topic>Mitotic Index</topic><topic>Mutagenicity Tests</topic><topic>Mutagens - administration & dosage</topic><topic>Mutagens - toxicity</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lima, P.D.L.</creatorcontrib><creatorcontrib>Vasconcellos, M.C.</creatorcontrib><creatorcontrib>Bahia, M.O.</creatorcontrib><creatorcontrib>Montenegro, R.C.</creatorcontrib><creatorcontrib>Pessoa, C.O.</creatorcontrib><creatorcontrib>Costa-Lotufo, L.V.</creatorcontrib><creatorcontrib>Moraes, M.O.</creatorcontrib><creatorcontrib>Burbano, R.R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Toxicology in vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lima, P.D.L.</au><au>Vasconcellos, M.C.</au><au>Bahia, M.O.</au><au>Montenegro, R.C.</au><au>Pessoa, C.O.</au><au>Costa-Lotufo, L.V.</au><au>Moraes, M.O.</au><au>Burbano, R.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotoxic and cytotoxic effects of manganese chloride in cultured human lymphocytes treated in different phases of cell cycle</atitle><jtitle>Toxicology in vitro</jtitle><addtitle>Toxicol In Vitro</addtitle><date>2008-06</date><risdate>2008</risdate><volume>22</volume><issue>4</issue><spage>1032</spage><epage>1037</epage><pages>1032-1037</pages><issn>0887-2333</issn><eissn>1879-3177</eissn><abstract>Manganese (Mn) has a natural occurrence and is necessary during the initial periods of the development. However, in high concentrations, Mn can be related to neurodegenerative disorders. The aim of the present study was to evaluate the mutagenic potential of manganese chloride (MnCl2·4H2O). Comet assay and chromosome aberrations analysis were applied to determine the DNA-damaging and clastogenic effects of MnCl2·4H2O. Cultured human lymphocytes were treated with 15, 20 and 25μM manganese chloride during the G1, G1/S, S (pulses of 1 and 6h), and G2 phases of the cell cycle. All tested concentrations were cytotoxic and reduced significantly the mitotic index in G1, G1/S and S (1 and 6h) treatments, while in G2 treatment only the higher concentrations (20 and 25μM) showed cytotoxic effects. Clastogenicity and DNA damage were found only in treatments with the highest concentration (25μM). Chromosome aberrations were found exclusively in the G2 phase of the cell cycle. The absence of polyploidy in mitosis, suggests that manganese does not affect the formation of the mitotic spindle with the concentrations tested. The genotoxicity found in G2 phase and in the comet assay can be related to the short time of treatment in both cases.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>18258413</pmid><doi>10.1016/j.tiv.2007.12.011</doi><tpages>6</tpages></addata></record> |
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| subjects | Cell Cycle - drug effects Cells, Cultured Chlorides - administration & dosage Chlorides - toxicity Chromosome aberration Chromosome Aberrations - drug effects Comet Assay Environmental Pollutants - administration & dosage Environmental Pollutants - toxicity Humans Lymphocytes - drug effects Lymphocytes - metabolism Manganese chloride Manganese Compounds - administration & dosage Mitotic Index Mutagenicity Tests Mutagens - administration & dosage Mutagens - toxicity Time Factors |
| title | Genotoxic and cytotoxic effects of manganese chloride in cultured human lymphocytes treated in different phases of cell cycle |
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