Binding of cholera toxin B subunit to intestinal epithelial cells

We have prepared 125I-labeled cholera toxin B subunit (125I-labeled CT-B, a specific activity of 98Ci/mmol) and found that it binds to rat IEC-6 and human Caco-2 intestinal epithelial cells with high affinity (Kd 3.6 and 3.7nM, respectively). The binding of labeled protein was completely inhibited b...

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Veröffentlicht in:	Toxicology in vitro 2018-03, Vol.47, p.269-273
Hauptverfasser:	Navolotskaya, Elena V., Sadovnikov, Vladimir B., Lipkin, Valery M., Zav'yalov, Vladimir P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Amino acid sequence Binding Cells Cholera Cholera toxin Cholera toxin B subunit Epithelial cells Guanylate cyclase Infections Interferon Interferon-α Intestinal epithelial cells Intestine Nitric oxide Peptides Protein: Peptide Proteins Public health Receptor Studies Thymosin-α1 Toxicity Waterborne diseases
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