Improved Procedure for Induction of the Androgenetic Doubled Haploids in Zebrafish

Androgenesis is useful for induction of doubled haploids from male genetic resources and contributes to the restoration of individuals from cryopreserved sperm. Here, we determined the suitable conditions for egg in vitro preservation and the suitable dose of UV irradiation for genetic inactivation...

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Veröffentlicht in:	Zebrafish 2018-02, Vol.15 (1), p.33-44
Hauptverfasser:	Endoh, Mitsuru, Fujimoto, Takafumi, Yamaha, Etsuro, Arai, Katsutoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Androgenesis Animal embryos Biological fertilization Bovine serum albumin Cryopreservation Danio rerio Deactivation Eggs Embryos Fertilization Freshwater fishes Genetic resources Genotyping Haploidy Heat shock Homozygosity Inactivation Irradiation Microsatellites Nuclei Nucleus Original Articles Paternity Radiation dosage Restoration Serum Serum albumin Solutions Spermatozoa Ultraviolet radiation Yields Zebrafish
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