Improved Procedure for Induction of the Androgenetic Doubled Haploids in Zebrafish
Androgenesis is useful for induction of doubled haploids from male genetic resources and contributes to the restoration of individuals from cryopreserved sperm. Here, we determined the suitable conditions for egg in vitro preservation and the suitable dose of UV irradiation for genetic inactivation...
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| Veröffentlicht in: | Zebrafish 2018-02, Vol.15 (1), p.33-44 |
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| creator | Endoh, Mitsuru Fujimoto, Takafumi Yamaha, Etsuro Arai, Katsutoshi |
| description | Androgenesis is useful for induction of doubled haploids from male genetic resources and contributes to the restoration of individuals from cryopreserved sperm. Here, we determined the suitable conditions for egg
in vitro
preservation and the suitable dose of UV irradiation for genetic inactivation of the egg nucleus, and established an improved procedure for induction of androgenetic-doubled haploids in zebrafish. The suitable solution for egg preservation was evaluated by the fertilization rate using different types of solutions or conditions. Hank's solution with 0.5% bovine serum albumin (pH8.0) was suitable for the preservation of zebrafish eggs. In addition, we discovered an improvement of fertilization rates by temporal preservation of ovulated eggs in the suitable solution. UV irradiation of eggs at 50–75 mJ/cm
2
induced haploid embryos. Microsatellite genotyping using eight loci revealed the paternity and homozygosity of the putative androgenetic doubled haploids. The yield rate of androgenetic doubled haploids, which were induced by UV irradiation and heat shock, ranged from 0.4% to 10.7%. |
| doi_str_mv | 10.1089/zeb.2017.1482 |
| format | Article |
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in vitro
preservation and the suitable dose of UV irradiation for genetic inactivation of the egg nucleus, and established an improved procedure for induction of androgenetic-doubled haploids in zebrafish. The suitable solution for egg preservation was evaluated by the fertilization rate using different types of solutions or conditions. Hank's solution with 0.5% bovine serum albumin (pH8.0) was suitable for the preservation of zebrafish eggs. In addition, we discovered an improvement of fertilization rates by temporal preservation of ovulated eggs in the suitable solution. UV irradiation of eggs at 50–75 mJ/cm
2
induced haploid embryos. Microsatellite genotyping using eight loci revealed the paternity and homozygosity of the putative androgenetic doubled haploids. The yield rate of androgenetic doubled haploids, which were induced by UV irradiation and heat shock, ranged from 0.4% to 10.7%.</description><identifier>ISSN: 1545-8547</identifier><identifier>EISSN: 1557-8542</identifier><identifier>DOI: 10.1089/zeb.2017.1482</identifier><identifier>PMID: 29261026</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Androgenesis ; Animal embryos ; Biological fertilization ; Bovine serum albumin ; Cryopreservation ; Danio rerio ; Deactivation ; Eggs ; Embryos ; Fertilization ; Freshwater fishes ; Genetic resources ; Genotyping ; Haploidy ; Heat shock ; Homozygosity ; Inactivation ; Irradiation ; Microsatellites ; Nuclei ; Nucleus ; Original Articles ; Paternity ; Radiation dosage ; Restoration ; Serum ; Serum albumin ; Solutions ; Spermatozoa ; Ultraviolet radiation ; Yields ; Zebrafish</subject><ispartof>Zebrafish, 2018-02, Vol.15 (1), p.33-44</ispartof><rights>2018, Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2018, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-fb2047e97c0f3c46bdf2557099cbd9acd8eef13c91d81cb1e090288b529b4bf13</citedby><cites>FETCH-LOGICAL-c431t-fb2047e97c0f3c46bdf2557099cbd9acd8eef13c91d81cb1e090288b529b4bf13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29261026$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Endoh, Mitsuru</creatorcontrib><creatorcontrib>Fujimoto, Takafumi</creatorcontrib><creatorcontrib>Yamaha, Etsuro</creatorcontrib><creatorcontrib>Arai, Katsutoshi</creatorcontrib><title>Improved Procedure for Induction of the Androgenetic Doubled Haploids in Zebrafish</title><title>Zebrafish</title><addtitle>Zebrafish</addtitle><description>Androgenesis is useful for induction of doubled haploids from male genetic resources and contributes to the restoration of individuals from cryopreserved sperm. Here, we determined the suitable conditions for egg
in vitro
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2
induced haploid embryos. Microsatellite genotyping using eight loci revealed the paternity and homozygosity of the putative androgenetic doubled haploids. The yield rate of androgenetic doubled haploids, which were induced by UV irradiation and heat shock, ranged from 0.4% to 10.7%.</description><subject>Androgenesis</subject><subject>Animal embryos</subject><subject>Biological fertilization</subject><subject>Bovine serum albumin</subject><subject>Cryopreservation</subject><subject>Danio rerio</subject><subject>Deactivation</subject><subject>Eggs</subject><subject>Embryos</subject><subject>Fertilization</subject><subject>Freshwater fishes</subject><subject>Genetic resources</subject><subject>Genotyping</subject><subject>Haploidy</subject><subject>Heat shock</subject><subject>Homozygosity</subject><subject>Inactivation</subject><subject>Irradiation</subject><subject>Microsatellites</subject><subject>Nuclei</subject><subject>Nucleus</subject><subject>Original Articles</subject><subject>Paternity</subject><subject>Radiation dosage</subject><subject>Restoration</subject><subject>Serum</subject><subject>Serum albumin</subject><subject>Solutions</subject><subject>Spermatozoa</subject><subject>Ultraviolet radiation</subject><subject>Yields</subject><subject>Zebrafish</subject><issn>1545-8547</issn><issn>1557-8542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqFkD1PwzAQhi0EgvIxsiJLLCwptuPU8ViVr0qVQAgWlii2zzQotYudIMGvx1ELAwuTT77nXt09CJ1SMqaklJdfoMaMUDGmvGQ7aESLQmRlwdnuUPNiqMUBOozxjZA8lznfRwdMsgklbDJCj_PVOvgPMPgheA2mD4CtD3juTK-7xjvsLe6WgKfOBP8KDrpG4yvfqzbN3NXr1jcm4sbhF1Chtk1cHqM9W7cRTrbvEXq-uX6a3WWL-9v5bLrINM9pl1nFCBcghSY213yijGVpdyKlVkbW2pQAluZaUlNSrSgQSVhZqoJJxVXqHKGLTW464L2H2FWrJmpo29qB72NFpZBUkEKyhJ7_Qd98H1zaLlGpLwRlPFHZhtLBxxjAVuvQrOrwWVFSDbKrJLsaZFeD7MSfbVN7tQLzS__YTUC-AYbv2rm2AQWh-yf2Gzs0i0g</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Endoh, Mitsuru</creator><creator>Fujimoto, Takafumi</creator><creator>Yamaha, Etsuro</creator><creator>Arai, Katsutoshi</creator><general>Mary Ann Liebert, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20180201</creationdate><title>Improved Procedure for Induction of the Androgenetic Doubled Haploids in Zebrafish</title><author>Endoh, Mitsuru ; Fujimoto, Takafumi ; Yamaha, Etsuro ; Arai, Katsutoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-fb2047e97c0f3c46bdf2557099cbd9acd8eef13c91d81cb1e090288b529b4bf13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Androgenesis</topic><topic>Animal embryos</topic><topic>Biological fertilization</topic><topic>Bovine serum albumin</topic><topic>Cryopreservation</topic><topic>Danio rerio</topic><topic>Deactivation</topic><topic>Eggs</topic><topic>Embryos</topic><topic>Fertilization</topic><topic>Freshwater fishes</topic><topic>Genetic resources</topic><topic>Genotyping</topic><topic>Haploidy</topic><topic>Heat shock</topic><topic>Homozygosity</topic><topic>Inactivation</topic><topic>Irradiation</topic><topic>Microsatellites</topic><topic>Nuclei</topic><topic>Nucleus</topic><topic>Original Articles</topic><topic>Paternity</topic><topic>Radiation dosage</topic><topic>Restoration</topic><topic>Serum</topic><topic>Serum albumin</topic><topic>Solutions</topic><topic>Spermatozoa</topic><topic>Ultraviolet radiation</topic><topic>Yields</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Endoh, Mitsuru</creatorcontrib><creatorcontrib>Fujimoto, Takafumi</creatorcontrib><creatorcontrib>Yamaha, Etsuro</creatorcontrib><creatorcontrib>Arai, Katsutoshi</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Zebrafish</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Endoh, Mitsuru</au><au>Fujimoto, Takafumi</au><au>Yamaha, Etsuro</au><au>Arai, Katsutoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved Procedure for Induction of the Androgenetic Doubled Haploids in Zebrafish</atitle><jtitle>Zebrafish</jtitle><addtitle>Zebrafish</addtitle><date>2018-02-01</date><risdate>2018</risdate><volume>15</volume><issue>1</issue><spage>33</spage><epage>44</epage><pages>33-44</pages><issn>1545-8547</issn><eissn>1557-8542</eissn><abstract>Androgenesis is useful for induction of doubled haploids from male genetic resources and contributes to the restoration of individuals from cryopreserved sperm. Here, we determined the suitable conditions for egg
in vitro
preservation and the suitable dose of UV irradiation for genetic inactivation of the egg nucleus, and established an improved procedure for induction of androgenetic-doubled haploids in zebrafish. The suitable solution for egg preservation was evaluated by the fertilization rate using different types of solutions or conditions. Hank's solution with 0.5% bovine serum albumin (pH8.0) was suitable for the preservation of zebrafish eggs. In addition, we discovered an improvement of fertilization rates by temporal preservation of ovulated eggs in the suitable solution. UV irradiation of eggs at 50–75 mJ/cm
2
induced haploid embryos. Microsatellite genotyping using eight loci revealed the paternity and homozygosity of the putative androgenetic doubled haploids. The yield rate of androgenetic doubled haploids, which were induced by UV irradiation and heat shock, ranged from 0.4% to 10.7%.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>29261026</pmid><doi>10.1089/zeb.2017.1482</doi><tpages>12</tpages></addata></record> |
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| subjects | Androgenesis Animal embryos Biological fertilization Bovine serum albumin Cryopreservation Danio rerio Deactivation Eggs Embryos Fertilization Freshwater fishes Genetic resources Genotyping Haploidy Heat shock Homozygosity Inactivation Irradiation Microsatellites Nuclei Nucleus Original Articles Paternity Radiation dosage Restoration Serum Serum albumin Solutions Spermatozoa Ultraviolet radiation Yields Zebrafish |
| title | Improved Procedure for Induction of the Androgenetic Doubled Haploids in Zebrafish |
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