Antibody Microarray Detection of Escherichia coli O157:H7:  Quantification, Assay Limitations, and Capture Efficiency

A sandwich fluorescent immunoassay in a microarray format was used to capture and detect E. coli O157:H7. Here, we explored quantitative aspects, limitations, and capture efficiency of the assay. When biotinylated capture antibodies were used, the signal generated was higher (over 5-fold higher with...

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Veröffentlicht in:	Analytical chemistry (Washington) 2006-09, Vol.78 (18), p.6601-6607
Hauptverfasser:	GEHRING, Andrew G., ALBIN, David M., BHUNIA, Arun K., REED, Sue A., TU, Shu-I, UKNALIS, Joseph
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Antibodies, Bacterial - chemistry Arrays Colony Count, Microbial E coli Escherichia coli Escherichia coli O157 - isolation & purification Fluorescence Food Microbiology Immunoassay Immunoglobulins Microscopy, Fluorescence Protein Array Analysis - methods Sensitivity and Specificity
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