The determination of earthworm species sensitivity differences to cadmium genotoxicity using the comet assay
The concept of species sensitivity differences is important in ecotoxicology and environmental risk assessment, but testing usually focuses on lethality of toxicants. The effects on the suborganismal level are mostly ignored; therefore, the present study assessed a biomarker of genotoxicity (the alk...
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| Veröffentlicht in: | Ecotoxicology and environmental safety 2007-07, Vol.67 (3), p.361-368 |
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| creator | Fourie, F. Reinecke, S.A. Reinecke, A.J. |
| description | The concept of species sensitivity differences is important in ecotoxicology and environmental risk assessment, but testing usually focuses on lethality of toxicants. The effects on the suborganismal level are mostly ignored; therefore, the present study assessed a biomarker of genotoxicity (the alkaline comet assay) to compare species sensitivities. Five earthworm species (
Amynthas diffringens,
Aporrectodea caliginosa,
Dendrodrilus rubidus,
Eisenia fetida and
Microchaetus benhami) were exposed for 48
h to sublethal concentrations of cadmium sulphate in reconstituted soil water and DNA integrity was evaluated with the parameter Tail DNA %. Significant amounts of DNA damage were detected in three (
A. caliginosa,
D. rubidus and
E. fetida) species.
E. fetida exhibited the highest level of DNA damage, although
D. rubidus showed the highest increase (3-fold) in DNA damage from the control. All exposed earthworms accumulated Cd, although body loads did not correspond with DNA damage levels; most of the Cd was probably sequestrated and rendered harmless. |
| doi_str_mv | 10.1016/j.ecoenv.2006.10.005 |
| format | Article |
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Amynthas diffringens,
Aporrectodea caliginosa,
Dendrodrilus rubidus,
Eisenia fetida and
Microchaetus benhami) were exposed for 48
h to sublethal concentrations of cadmium sulphate in reconstituted soil water and DNA integrity was evaluated with the parameter Tail DNA %. Significant amounts of DNA damage were detected in three (
A. caliginosa,
D. rubidus and
E. fetida) species.
E. fetida exhibited the highest level of DNA damage, although
D. rubidus showed the highest increase (3-fold) in DNA damage from the control. All exposed earthworms accumulated Cd, although body loads did not correspond with DNA damage levels; most of the Cd was probably sequestrated and rendered harmless.</description><identifier>ISSN: 0147-6513</identifier><identifier>EISSN: 1090-2414</identifier><identifier>DOI: 10.1016/j.ecoenv.2006.10.005</identifier><identifier>PMID: 17173970</identifier><identifier>CODEN: EESADV</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animal, plant and microbial ecology ; Animals ; Aporrectodea caliginosa ; Applied ecology ; Assays ; Biological and medical sciences ; Cadmium ; Cadmium - toxicity ; Comet assay ; Comet Assay - instrumentation ; Comet Assay - methods ; DNA Damage ; Earthworms ; Ecotoxicology, biological effects of pollution ; Environmental Monitoring ; Fundamental and applied biological sciences. Psychology ; General aspects ; Oligochaeta - classification ; Oligochaeta - drug effects ; Oligochaeta - physiology ; Risk Assessment ; Single cell gel electrophoresis assay ; Soil Pollutants - toxicity ; Species sensitivity ; Species Specificity</subject><ispartof>Ecotoxicology and environmental safety, 2007-07, Vol.67 (3), p.361-368</ispartof><rights>2006 Elsevier Inc.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-2a89796b5362997c85ce8cd7134118c9331bc2c3e21d20e021f7b5207fe145a83</citedby><cites>FETCH-LOGICAL-c452t-2a89796b5362997c85ce8cd7134118c9331bc2c3e21d20e021f7b5207fe145a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0147651306002028$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18875905$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17173970$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fourie, F.</creatorcontrib><creatorcontrib>Reinecke, S.A.</creatorcontrib><creatorcontrib>Reinecke, A.J.</creatorcontrib><title>The determination of earthworm species sensitivity differences to cadmium genotoxicity using the comet assay</title><title>Ecotoxicology and environmental safety</title><addtitle>Ecotoxicol Environ Saf</addtitle><description>The concept of species sensitivity differences is important in ecotoxicology and environmental risk assessment, but testing usually focuses on lethality of toxicants. The effects on the suborganismal level are mostly ignored; therefore, the present study assessed a biomarker of genotoxicity (the alkaline comet assay) to compare species sensitivities. Five earthworm species (
Amynthas diffringens,
Aporrectodea caliginosa,
Dendrodrilus rubidus,
Eisenia fetida and
Microchaetus benhami) were exposed for 48
h to sublethal concentrations of cadmium sulphate in reconstituted soil water and DNA integrity was evaluated with the parameter Tail DNA %. Significant amounts of DNA damage were detected in three (
A. caliginosa,
D. rubidus and
E. fetida) species.
E. fetida exhibited the highest level of DNA damage, although
D. rubidus showed the highest increase (3-fold) in DNA damage from the control. All exposed earthworms accumulated Cd, although body loads did not correspond with DNA damage levels; most of the Cd was probably sequestrated and rendered harmless.</description><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Aporrectodea caliginosa</subject><subject>Applied ecology</subject><subject>Assays</subject><subject>Biological and medical sciences</subject><subject>Cadmium</subject><subject>Cadmium - toxicity</subject><subject>Comet assay</subject><subject>Comet Assay - instrumentation</subject><subject>Comet Assay - methods</subject><subject>DNA Damage</subject><subject>Earthworms</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Environmental Monitoring</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Oligochaeta - classification</subject><subject>Oligochaeta - drug effects</subject><subject>Oligochaeta - physiology</subject><subject>Risk Assessment</subject><subject>Single cell gel electrophoresis assay</subject><subject>Soil Pollutants - toxicity</subject><subject>Species sensitivity</subject><subject>Species Specificity</subject><issn>0147-6513</issn><issn>1090-2414</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGLEzEYhoMobrf6D0Ry0dvUL8lkkrkIsugqLHhZzyHNfLObMpPUJFPtvze1hb3pKfDyfC_hfQh5w2DDgHUfdht0EcNhwwG6Gm0A5DOyYtBDw1vWPicrYK1qOsnEFbnOeQcAAqR8Sa6YYkr0ClZkun9EOmDBNPtgi4-BxpGiTeXxV0wzzXt0HjPNGLIv_uDLkQ5-HDFhcDUvkTo7zH6Z6QOGWOJv707Mkn14oKWWuzhjoTZne3xFXox2yvj68q7Jjy-f72--Nnffb7_dfLprXCt5abjVveq7rRQd73vltHSo3aCYaBnTrheCbR13AjkbOCBwNqqt5KBGZK20WqzJ-3PvPsWfC-ZiZp8dTpMNGJdsWK-0kLz7P9hq6ETdak3aM-hSzDnhaPbJzzYdDQNz0mF25qzDnHSc0qqjnr299C_bGYeno8v-FXh3AWx2dhqTDc7nJ05rJfu_RR_PHNbZDh6TyVVLNTD4hK6YIfp__-QPOb2rkA</recordid><startdate>20070701</startdate><enddate>20070701</enddate><creator>Fourie, F.</creator><creator>Reinecke, S.A.</creator><creator>Reinecke, A.J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7TM</scope><scope>7TV</scope><scope>7U7</scope></search><sort><creationdate>20070701</creationdate><title>The determination of earthworm species sensitivity differences to cadmium genotoxicity using the comet assay</title><author>Fourie, F. ; Reinecke, S.A. ; Reinecke, A.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-2a89796b5362997c85ce8cd7134118c9331bc2c3e21d20e021f7b5207fe145a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Aporrectodea caliginosa</topic><topic>Applied ecology</topic><topic>Assays</topic><topic>Biological and medical sciences</topic><topic>Cadmium</topic><topic>Cadmium - toxicity</topic><topic>Comet assay</topic><topic>Comet Assay - instrumentation</topic><topic>Comet Assay - methods</topic><topic>DNA Damage</topic><topic>Earthworms</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Environmental Monitoring</topic><topic>Fundamental and applied biological sciences. 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The effects on the suborganismal level are mostly ignored; therefore, the present study assessed a biomarker of genotoxicity (the alkaline comet assay) to compare species sensitivities. Five earthworm species (
Amynthas diffringens,
Aporrectodea caliginosa,
Dendrodrilus rubidus,
Eisenia fetida and
Microchaetus benhami) were exposed for 48
h to sublethal concentrations of cadmium sulphate in reconstituted soil water and DNA integrity was evaluated with the parameter Tail DNA %. Significant amounts of DNA damage were detected in three (
A. caliginosa,
D. rubidus and
E. fetida) species.
E. fetida exhibited the highest level of DNA damage, although
D. rubidus showed the highest increase (3-fold) in DNA damage from the control. All exposed earthworms accumulated Cd, although body loads did not correspond with DNA damage levels; most of the Cd was probably sequestrated and rendered harmless.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>17173970</pmid><doi>10.1016/j.ecoenv.2006.10.005</doi><tpages>8</tpages></addata></record> |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_19783526 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animal, plant and microbial ecology Animals Aporrectodea caliginosa Applied ecology Assays Biological and medical sciences Cadmium Cadmium - toxicity Comet assay Comet Assay - instrumentation Comet Assay - methods DNA Damage Earthworms Ecotoxicology, biological effects of pollution Environmental Monitoring Fundamental and applied biological sciences. Psychology General aspects Oligochaeta - classification Oligochaeta - drug effects Oligochaeta - physiology Risk Assessment Single cell gel electrophoresis assay Soil Pollutants - toxicity Species sensitivity Species Specificity |
| title | The determination of earthworm species sensitivity differences to cadmium genotoxicity using the comet assay |
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