First report of aphantoxins in China—waterblooms of toxigenic Aphanizomenon flos-aquae in Lake Dianchi
The oligohaline cyanobacterium Aphanizomenon flos-aquae (L.) Ralfs ( A. flos-aquae) has been reported in several countries to produce paralytic shellfish poisons (PSPs) or protracted toxic effects. In the past years, A. flos-aquae blooms have occurred annually in the eutrophic Lake Dianchi (300 km 2...
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| Veröffentlicht in: | Ecotoxicology and environmental safety 2006-09, Vol.65 (1), p.84-92 |
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| creator | Liu, Yongmei Chen, Wei Li, Dunhai Shen, Yinwu Li, Genbao Liu, Yongding |
| description | The oligohaline cyanobacterium
Aphanizomenon flos-aquae (L.) Ralfs (
A. flos-aquae) has been reported in several countries to produce paralytic shellfish poisons (PSPs) or protracted toxic effects. In the past years,
A. flos-aquae blooms have occurred annually in the eutrophic Lake Dianchi (300
km
2 in area, located in southwestern China). Material from natural blooms dominated by
A. flos-aquae was collected and lyophilized. Acute toxicity testing was performed by mouse bioassay using extracts from the lyophilized material. Clear symptoms of PSPs intoxications were observed. To confirm the production of PSPs, a strain of
A. flos-aquae (DC-1) was isolated and maintained in culture. Histopathological effects were studied by examining the organ damages using transmission electron microscopy (TEM). Slight hepatocytic damage with swollen mitochondria was found. The ultrastructural pulmonary lesions were characterized by distortied nuclei and indenting of karyotheca, together with degeneration and tumefaction of mitochondria and endoplasmic reticulum. Control animals injected with acetic acid did not exhibit histopathological damage in any organ. Toxic effects of cultured algal cells on enzymatic systems in the mouse were studied using sublethal doses of extracts. Significant glutathione-
S-transferase (GST) and lactate dehydrogenase (LDH) increases, together with decrease of the glutathione (GSH) level, were measured. These results indicated a potential role of PSPs intoxicating and metabolizing in the test animals. HPLC-FLD and LC/MS analysis of extracts from cultured material demonstrated the PSP toxins produced by
A. flos-aquae bloom. To the best of our knowledge, this is the first study reporting chemically and toxicologically confirmed PSP toxins related to
A. flos-aquae in China. |
| doi_str_mv | 10.1016/j.ecoenv.2005.06.012 |
| format | Article |
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Aphanizomenon flos-aquae (L.) Ralfs (
A. flos-aquae) has been reported in several countries to produce paralytic shellfish poisons (PSPs) or protracted toxic effects. In the past years,
A. flos-aquae blooms have occurred annually in the eutrophic Lake Dianchi (300
km
2 in area, located in southwestern China). Material from natural blooms dominated by
A. flos-aquae was collected and lyophilized. Acute toxicity testing was performed by mouse bioassay using extracts from the lyophilized material. Clear symptoms of PSPs intoxications were observed. To confirm the production of PSPs, a strain of
A. flos-aquae (DC-1) was isolated and maintained in culture. Histopathological effects were studied by examining the organ damages using transmission electron microscopy (TEM). Slight hepatocytic damage with swollen mitochondria was found. The ultrastructural pulmonary lesions were characterized by distortied nuclei and indenting of karyotheca, together with degeneration and tumefaction of mitochondria and endoplasmic reticulum. Control animals injected with acetic acid did not exhibit histopathological damage in any organ. Toxic effects of cultured algal cells on enzymatic systems in the mouse were studied using sublethal doses of extracts. Significant glutathione-
S-transferase (GST) and lactate dehydrogenase (LDH) increases, together with decrease of the glutathione (GSH) level, were measured. These results indicated a potential role of PSPs intoxicating and metabolizing in the test animals. HPLC-FLD and LC/MS analysis of extracts from cultured material demonstrated the PSP toxins produced by
A. flos-aquae bloom. To the best of our knowledge, this is the first study reporting chemically and toxicologically confirmed PSP toxins related to
A. flos-aquae in China.</description><identifier>ISSN: 0147-6513</identifier><identifier>EISSN: 1090-2414</identifier><identifier>DOI: 10.1016/j.ecoenv.2005.06.012</identifier><identifier>PMID: 16289338</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Aphanizomenon - physiology ; Aphanizomenon flos-aquae ; Bacterial Toxins - chemistry ; Bacterial Toxins - toxicity ; Biological Assay ; Cell Nucleolus - ultrastructure ; Cell Nucleus - ultrastructure ; China ; Chromatography, High Pressure Liquid ; Chromatography, Liquid ; Cyanobacterial bloom ; Cyanophyta ; Eutrophication - physiology ; Fresh Water - chemistry ; Fresh Water - microbiology ; Freshwater ; Glutathione - metabolism ; Glutathione Transferase - metabolism ; HPLC ; L-Lactate Dehydrogenase - metabolism ; Lake Dianchi ; LC/MS ; Liver - ultrastructure ; Male ; Marine Toxins - chemistry ; Marine Toxins - toxicity ; Mass Spectrometry ; Mice ; Mitochondria - ultrastructure ; Mouse bioassay ; Paralytic shellfish poisons ; Ralfs ; Toxicology</subject><ispartof>Ecotoxicology and environmental safety, 2006-09, Vol.65 (1), p.84-92</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-577906327e5e72ac696ce053fd89809a89c298065368cd94f70c9fa4ca905f2d3</citedby><cites>FETCH-LOGICAL-c415t-577906327e5e72ac696ce053fd89809a89c298065368cd94f70c9fa4ca905f2d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0147651305001600$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16289338$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Yongmei</creatorcontrib><creatorcontrib>Chen, Wei</creatorcontrib><creatorcontrib>Li, Dunhai</creatorcontrib><creatorcontrib>Shen, Yinwu</creatorcontrib><creatorcontrib>Li, Genbao</creatorcontrib><creatorcontrib>Liu, Yongding</creatorcontrib><title>First report of aphantoxins in China—waterblooms of toxigenic Aphanizomenon flos-aquae in Lake Dianchi</title><title>Ecotoxicology and environmental safety</title><addtitle>Ecotoxicol Environ Saf</addtitle><description>The oligohaline cyanobacterium
Aphanizomenon flos-aquae (L.) Ralfs (
A. flos-aquae) has been reported in several countries to produce paralytic shellfish poisons (PSPs) or protracted toxic effects. In the past years,
A. flos-aquae blooms have occurred annually in the eutrophic Lake Dianchi (300
km
2 in area, located in southwestern China). Material from natural blooms dominated by
A. flos-aquae was collected and lyophilized. Acute toxicity testing was performed by mouse bioassay using extracts from the lyophilized material. Clear symptoms of PSPs intoxications were observed. To confirm the production of PSPs, a strain of
A. flos-aquae (DC-1) was isolated and maintained in culture. Histopathological effects were studied by examining the organ damages using transmission electron microscopy (TEM). Slight hepatocytic damage with swollen mitochondria was found. The ultrastructural pulmonary lesions were characterized by distortied nuclei and indenting of karyotheca, together with degeneration and tumefaction of mitochondria and endoplasmic reticulum. Control animals injected with acetic acid did not exhibit histopathological damage in any organ. Toxic effects of cultured algal cells on enzymatic systems in the mouse were studied using sublethal doses of extracts. Significant glutathione-
S-transferase (GST) and lactate dehydrogenase (LDH) increases, together with decrease of the glutathione (GSH) level, were measured. These results indicated a potential role of PSPs intoxicating and metabolizing in the test animals. HPLC-FLD and LC/MS analysis of extracts from cultured material demonstrated the PSP toxins produced by
A. flos-aquae bloom. To the best of our knowledge, this is the first study reporting chemically and toxicologically confirmed PSP toxins related to
A. flos-aquae in China.</description><subject>Animals</subject><subject>Aphanizomenon - physiology</subject><subject>Aphanizomenon flos-aquae</subject><subject>Bacterial Toxins - chemistry</subject><subject>Bacterial Toxins - toxicity</subject><subject>Biological Assay</subject><subject>Cell Nucleolus - ultrastructure</subject><subject>Cell Nucleus - ultrastructure</subject><subject>China</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Liquid</subject><subject>Cyanobacterial bloom</subject><subject>Cyanophyta</subject><subject>Eutrophication - physiology</subject><subject>Fresh Water - chemistry</subject><subject>Fresh Water - microbiology</subject><subject>Freshwater</subject><subject>Glutathione - metabolism</subject><subject>Glutathione Transferase - metabolism</subject><subject>HPLC</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Lake Dianchi</subject><subject>LC/MS</subject><subject>Liver - ultrastructure</subject><subject>Male</subject><subject>Marine Toxins - chemistry</subject><subject>Marine Toxins - toxicity</subject><subject>Mass Spectrometry</subject><subject>Mice</subject><subject>Mitochondria - ultrastructure</subject><subject>Mouse bioassay</subject><subject>Paralytic shellfish poisons</subject><subject>Ralfs</subject><subject>Toxicology</subject><issn>0147-6513</issn><issn>1090-2414</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi1ERZfCGyDIqbeEsR078QWpWlpAWokD9Gy5zrjrJbG3dra0nHgInrBPQqKsxI3TzOH7f42-IeQNhYoCle93FdqI4b5iAKICWQFlz8iKgoKS1bR-TlZA66aUgvJT8jLnHQBwEOIFOaWStYrzdkW2Vz7lsUi4j2ksoivMfmvCGB98yIUPxXrrg3n6_eenGTHd9DEOeaZm4BaDt8XFzPtfccAQQ-H6mEtzdzA4hzfmBxYfvQl261-RE2f6jK-P84xcX11-X38uN18_fVlfbEpbUzGWomkUSM4aFNgwY6WSFkFw17WqBWVaZdm0SMFlaztVuwascqa2RoFwrONn5Hzp3ad4d8A86sFni31vAsZD1lQ1jVCKTWC9gDbFnBM6vU9-MOlRU9CzYb3Ti2E9G9Yg9WR4ir099h9uBuz-hY5KJ-DdAjgTtblNPuvrbwwoh-k1Emo-ER8WAicP9x6TztZjsNj5hHbUXfT_v-EvrGuZQw</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>Liu, Yongmei</creator><creator>Chen, Wei</creator><creator>Li, Dunhai</creator><creator>Shen, Yinwu</creator><creator>Li, Genbao</creator><creator>Liu, Yongding</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>20060901</creationdate><title>First report of aphantoxins in China—waterblooms of toxigenic Aphanizomenon flos-aquae in Lake Dianchi</title><author>Liu, Yongmei ; Chen, Wei ; Li, Dunhai ; Shen, Yinwu ; Li, Genbao ; Liu, Yongding</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-577906327e5e72ac696ce053fd89809a89c298065368cd94f70c9fa4ca905f2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Aphanizomenon - physiology</topic><topic>Aphanizomenon flos-aquae</topic><topic>Bacterial Toxins - chemistry</topic><topic>Bacterial Toxins - toxicity</topic><topic>Biological Assay</topic><topic>Cell Nucleolus - ultrastructure</topic><topic>Cell Nucleus - ultrastructure</topic><topic>China</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Liquid</topic><topic>Cyanobacterial bloom</topic><topic>Cyanophyta</topic><topic>Eutrophication - physiology</topic><topic>Fresh Water - chemistry</topic><topic>Fresh Water - microbiology</topic><topic>Freshwater</topic><topic>Glutathione - metabolism</topic><topic>Glutathione Transferase - metabolism</topic><topic>HPLC</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Lake Dianchi</topic><topic>LC/MS</topic><topic>Liver - ultrastructure</topic><topic>Male</topic><topic>Marine Toxins - chemistry</topic><topic>Marine Toxins - toxicity</topic><topic>Mass Spectrometry</topic><topic>Mice</topic><topic>Mitochondria - ultrastructure</topic><topic>Mouse bioassay</topic><topic>Paralytic shellfish poisons</topic><topic>Ralfs</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Yongmei</creatorcontrib><creatorcontrib>Chen, Wei</creatorcontrib><creatorcontrib>Li, Dunhai</creatorcontrib><creatorcontrib>Shen, Yinwu</creatorcontrib><creatorcontrib>Li, Genbao</creatorcontrib><creatorcontrib>Liu, Yongding</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Ecotoxicology and environmental safety</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Yongmei</au><au>Chen, Wei</au><au>Li, Dunhai</au><au>Shen, Yinwu</au><au>Li, Genbao</au><au>Liu, Yongding</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First report of aphantoxins in China—waterblooms of toxigenic Aphanizomenon flos-aquae in Lake Dianchi</atitle><jtitle>Ecotoxicology and environmental safety</jtitle><addtitle>Ecotoxicol Environ Saf</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>65</volume><issue>1</issue><spage>84</spage><epage>92</epage><pages>84-92</pages><issn>0147-6513</issn><eissn>1090-2414</eissn><abstract>The oligohaline cyanobacterium
Aphanizomenon flos-aquae (L.) Ralfs (
A. flos-aquae) has been reported in several countries to produce paralytic shellfish poisons (PSPs) or protracted toxic effects. In the past years,
A. flos-aquae blooms have occurred annually in the eutrophic Lake Dianchi (300
km
2 in area, located in southwestern China). Material from natural blooms dominated by
A. flos-aquae was collected and lyophilized. Acute toxicity testing was performed by mouse bioassay using extracts from the lyophilized material. Clear symptoms of PSPs intoxications were observed. To confirm the production of PSPs, a strain of
A. flos-aquae (DC-1) was isolated and maintained in culture. Histopathological effects were studied by examining the organ damages using transmission electron microscopy (TEM). Slight hepatocytic damage with swollen mitochondria was found. The ultrastructural pulmonary lesions were characterized by distortied nuclei and indenting of karyotheca, together with degeneration and tumefaction of mitochondria and endoplasmic reticulum. Control animals injected with acetic acid did not exhibit histopathological damage in any organ. Toxic effects of cultured algal cells on enzymatic systems in the mouse were studied using sublethal doses of extracts. Significant glutathione-
S-transferase (GST) and lactate dehydrogenase (LDH) increases, together with decrease of the glutathione (GSH) level, were measured. These results indicated a potential role of PSPs intoxicating and metabolizing in the test animals. HPLC-FLD and LC/MS analysis of extracts from cultured material demonstrated the PSP toxins produced by
A. flos-aquae bloom. To the best of our knowledge, this is the first study reporting chemically and toxicologically confirmed PSP toxins related to
A. flos-aquae in China.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>16289338</pmid><doi>10.1016/j.ecoenv.2005.06.012</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Ecotoxicology and environmental safety, 2006-09, Vol.65 (1), p.84-92 |
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| subjects | Animals Aphanizomenon - physiology Aphanizomenon flos-aquae Bacterial Toxins - chemistry Bacterial Toxins - toxicity Biological Assay Cell Nucleolus - ultrastructure Cell Nucleus - ultrastructure China Chromatography, High Pressure Liquid Chromatography, Liquid Cyanobacterial bloom Cyanophyta Eutrophication - physiology Fresh Water - chemistry Fresh Water - microbiology Freshwater Glutathione - metabolism Glutathione Transferase - metabolism HPLC L-Lactate Dehydrogenase - metabolism Lake Dianchi LC/MS Liver - ultrastructure Male Marine Toxins - chemistry Marine Toxins - toxicity Mass Spectrometry Mice Mitochondria - ultrastructure Mouse bioassay Paralytic shellfish poisons Ralfs Toxicology |
| title | First report of aphantoxins in China—waterblooms of toxigenic Aphanizomenon flos-aquae in Lake Dianchi |
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