Characterization of a collection of Enterobacter sakazakii isolates from environmental and food sources
Enterobacter sakazakii has emerged as a rare cause of neonatal meningitis, septicemia and enterocolitis. Contaminated infant milk formula (IMF) has been identified as one infection route. A small number of clinical outbreaks have been epidemiologically linked to IMF contaminated post-pasteurization...
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| Veröffentlicht in: | International journal of food microbiology 2006-07, Vol.110 (2), p.127-134 |
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| creator | Drudy, Denise O'Rourke, Michele Murphy, Mary Mullane, Niall R O'Mahony, Rebecca Kelly, Lorraine Fischer, Matthias Sanjaq, Suhad Shannon, Pauline Wall, Patrick O'Mahony, Micheál Whyte, Paul Fanning, Séamus |
| description | Enterobacter sakazakii has emerged as a rare cause of neonatal meningitis, septicemia and enterocolitis. Contaminated infant milk formula (IMF) has been identified as one infection route. A small number of clinical outbreaks have been epidemiologically linked to IMF contaminated post-pasteurization during manufacture and/or mishandled when reconstituted. Currently no agreed standardized typing protocol has been developed to trace
E. sakazakii. The objectives of this study were to apply biochemical and genetic methods to characterize 51 environmental and food
E. sakazakii isolates and 6
E. sakazakii type strains. Isolates were presumptively identified using biochemical profiles based on API 20E and ID32E methods and by culture on differential selective Druggan Forsythe Iversen (DFI) agar. Identification was subsequently confirmed by real time polymerase chain reaction (PCR).
All but one of the isolates was identified as
E. sakazakii by biochemical profiling. One isolate was identified as
Escherichia vulneris by ID 32E and as
Pantoea agglomerans by API 20E. All isolates produced green/blue colonies on DFI medium characteristic of this organism. Real time PCR could differentiate between
E. sakazakii, Enterobacter spp. and other
Enterobacteriacae. Analysis of RAPD banding patterns revealed 3 major clusters of
E. sakazakii. There was a large degree of diversity noted amongst the remaining isolates. Our findings indicate that RAPD may be applied as a useful and reliable tool for direct comparison of
E. sakazakii isolates providing traceability through the infant formula food chain. |
| doi_str_mv | 10.1016/j.ijfoodmicro.2006.02.008 |
| format | Article |
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E. sakazakii. The objectives of this study were to apply biochemical and genetic methods to characterize 51 environmental and food
E. sakazakii isolates and 6
E. sakazakii type strains. Isolates were presumptively identified using biochemical profiles based on API 20E and ID32E methods and by culture on differential selective Druggan Forsythe Iversen (DFI) agar. Identification was subsequently confirmed by real time polymerase chain reaction (PCR).
All but one of the isolates was identified as
E. sakazakii by biochemical profiling. One isolate was identified as
Escherichia vulneris by ID 32E and as
Pantoea agglomerans by API 20E. All isolates produced green/blue colonies on DFI medium characteristic of this organism. Real time PCR could differentiate between
E. sakazakii, Enterobacter spp. and other
Enterobacteriacae. Analysis of RAPD banding patterns revealed 3 major clusters of
E. sakazakii. There was a large degree of diversity noted amongst the remaining isolates. Our findings indicate that RAPD may be applied as a useful and reliable tool for direct comparison of
E. sakazakii isolates providing traceability through the infant formula food chain.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2006.02.008</identifier><identifier>PMID: 16730386</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biological and medical sciences ; Cluster Analysis ; Colony Count, Microbial ; Cronobacter sakazakii - classification ; Cronobacter sakazakii - genetics ; Cronobacter sakazakii - isolation & purification ; DNA, Bacterial - analysis ; Enterobacter ; Enterobacter sakazakii ; Enterobacteriacae ; Enterobacteriaceae ; Environmental Microbiology ; Escherichia ; Food Contamination - analysis ; Food industries ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Profiling ; Humans ; Infant ; Infant Food - microbiology ; Infant, Newborn ; Neonatal meningitis ; Oligonucleotide Array Sequence Analysis ; Pantoea agglomerans ; Phylogeny ; Polymerase Chain Reaction ; Random Amplified Polymorphic DNA Technique ; RAPD typing ; Sensitivity and Specificity ; Species Specificity</subject><ispartof>International journal of food microbiology, 2006-07, Vol.110 (2), p.127-134</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-f5b122bdbd02981376619decb790d60ca2d854a32d519c036be15d5f643c89c93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168160506001875$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17976888$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16730386$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Drudy, Denise</creatorcontrib><creatorcontrib>O'Rourke, Michele</creatorcontrib><creatorcontrib>Murphy, Mary</creatorcontrib><creatorcontrib>Mullane, Niall R</creatorcontrib><creatorcontrib>O'Mahony, Rebecca</creatorcontrib><creatorcontrib>Kelly, Lorraine</creatorcontrib><creatorcontrib>Fischer, Matthias</creatorcontrib><creatorcontrib>Sanjaq, Suhad</creatorcontrib><creatorcontrib>Shannon, Pauline</creatorcontrib><creatorcontrib>Wall, Patrick</creatorcontrib><creatorcontrib>O'Mahony, Micheál</creatorcontrib><creatorcontrib>Whyte, Paul</creatorcontrib><creatorcontrib>Fanning, Séamus</creatorcontrib><title>Characterization of a collection of Enterobacter sakazakii isolates from environmental and food sources</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Enterobacter sakazakii has emerged as a rare cause of neonatal meningitis, septicemia and enterocolitis. Contaminated infant milk formula (IMF) has been identified as one infection route. A small number of clinical outbreaks have been epidemiologically linked to IMF contaminated post-pasteurization during manufacture and/or mishandled when reconstituted. Currently no agreed standardized typing protocol has been developed to trace
E. sakazakii. The objectives of this study were to apply biochemical and genetic methods to characterize 51 environmental and food
E. sakazakii isolates and 6
E. sakazakii type strains. Isolates were presumptively identified using biochemical profiles based on API 20E and ID32E methods and by culture on differential selective Druggan Forsythe Iversen (DFI) agar. Identification was subsequently confirmed by real time polymerase chain reaction (PCR).
All but one of the isolates was identified as
E. sakazakii by biochemical profiling. One isolate was identified as
Escherichia vulneris by ID 32E and as
Pantoea agglomerans by API 20E. All isolates produced green/blue colonies on DFI medium characteristic of this organism. Real time PCR could differentiate between
E. sakazakii, Enterobacter spp. and other
Enterobacteriacae. Analysis of RAPD banding patterns revealed 3 major clusters of
E. sakazakii. There was a large degree of diversity noted amongst the remaining isolates. Our findings indicate that RAPD may be applied as a useful and reliable tool for direct comparison of
E. sakazakii isolates providing traceability through the infant formula food chain.</description><subject>Biological and medical sciences</subject><subject>Cluster Analysis</subject><subject>Colony Count, Microbial</subject><subject>Cronobacter sakazakii - classification</subject><subject>Cronobacter sakazakii - genetics</subject><subject>Cronobacter sakazakii - isolation & purification</subject><subject>DNA, Bacterial - analysis</subject><subject>Enterobacter</subject><subject>Enterobacter sakazakii</subject><subject>Enterobacteriacae</subject><subject>Enterobacteriaceae</subject><subject>Environmental Microbiology</subject><subject>Escherichia</subject><subject>Food Contamination - analysis</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant Food - microbiology</subject><subject>Infant, Newborn</subject><subject>Neonatal meningitis</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Pantoea agglomerans</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>RAPD typing</subject><subject>Sensitivity and Specificity</subject><subject>Species Specificity</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtv1DAQgC0EokvhLyBzgFvC2N449hGtykOqxAXO1sSegLdJXOxspfbX42UXlSOn0Wi-eX2MvRHQChD6_b6N-zGlMEefUysBdAuyBTBP2EaY3jZqq-Ep21TWNEJDd8FelLIHgE4peM4uhO4VKKM37MfuJ2b0K-X4gGtMC08jR-7TNJH_m18ttZ6GPxgveIMPeBMjjyVNuFLhY04zp-Uu5rTMtKw4cVwCP57ISzpkT-UlezbiVOjVOV6y7x-vvu0-N9dfP33Zfbhu_Nb0azN2g5ByCEMAaY1QvdbCBvJDbyFo8CiD6baoZOiE9aD0QKIL3ai3yhvrrbpk705zb3P6daCyujkWT9OEC6VDccL29XVpKmhPYFVYSqbR3eY4Y753AtzRstu7fyy7o2UH0lXLtff1eclhmCk8dp61VuDtGcDicRozLj6WR663vTbmOGh34qgquYuUXfGRFk8h5qrfhRT_45zfJhWjIQ</recordid><startdate>20060715</startdate><enddate>20060715</enddate><creator>Drudy, Denise</creator><creator>O'Rourke, Michele</creator><creator>Murphy, Mary</creator><creator>Mullane, Niall R</creator><creator>O'Mahony, Rebecca</creator><creator>Kelly, Lorraine</creator><creator>Fischer, Matthias</creator><creator>Sanjaq, Suhad</creator><creator>Shannon, Pauline</creator><creator>Wall, Patrick</creator><creator>O'Mahony, Micheál</creator><creator>Whyte, Paul</creator><creator>Fanning, Séamus</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20060715</creationdate><title>Characterization of a collection of Enterobacter sakazakii isolates from environmental and food sources</title><author>Drudy, Denise ; O'Rourke, Michele ; Murphy, Mary ; Mullane, Niall R ; O'Mahony, Rebecca ; Kelly, Lorraine ; Fischer, Matthias ; Sanjaq, Suhad ; Shannon, Pauline ; Wall, Patrick ; O'Mahony, Micheál ; Whyte, Paul ; Fanning, Séamus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-f5b122bdbd02981376619decb790d60ca2d854a32d519c036be15d5f643c89c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biological and medical sciences</topic><topic>Cluster Analysis</topic><topic>Colony Count, Microbial</topic><topic>Cronobacter sakazakii - classification</topic><topic>Cronobacter sakazakii - genetics</topic><topic>Cronobacter sakazakii - isolation & purification</topic><topic>DNA, Bacterial - analysis</topic><topic>Enterobacter</topic><topic>Enterobacter sakazakii</topic><topic>Enterobacteriacae</topic><topic>Enterobacteriaceae</topic><topic>Environmental Microbiology</topic><topic>Escherichia</topic><topic>Food Contamination - analysis</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant Food - microbiology</topic><topic>Infant, Newborn</topic><topic>Neonatal meningitis</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Pantoea agglomerans</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>RAPD typing</topic><topic>Sensitivity and Specificity</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Drudy, Denise</creatorcontrib><creatorcontrib>O'Rourke, Michele</creatorcontrib><creatorcontrib>Murphy, Mary</creatorcontrib><creatorcontrib>Mullane, Niall R</creatorcontrib><creatorcontrib>O'Mahony, Rebecca</creatorcontrib><creatorcontrib>Kelly, Lorraine</creatorcontrib><creatorcontrib>Fischer, Matthias</creatorcontrib><creatorcontrib>Sanjaq, Suhad</creatorcontrib><creatorcontrib>Shannon, Pauline</creatorcontrib><creatorcontrib>Wall, Patrick</creatorcontrib><creatorcontrib>O'Mahony, Micheál</creatorcontrib><creatorcontrib>Whyte, Paul</creatorcontrib><creatorcontrib>Fanning, Séamus</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Drudy, Denise</au><au>O'Rourke, Michele</au><au>Murphy, Mary</au><au>Mullane, Niall R</au><au>O'Mahony, Rebecca</au><au>Kelly, Lorraine</au><au>Fischer, Matthias</au><au>Sanjaq, Suhad</au><au>Shannon, Pauline</au><au>Wall, Patrick</au><au>O'Mahony, Micheál</au><au>Whyte, Paul</au><au>Fanning, Séamus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a collection of Enterobacter sakazakii isolates from environmental and food sources</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2006-07-15</date><risdate>2006</risdate><volume>110</volume><issue>2</issue><spage>127</spage><epage>134</epage><pages>127-134</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>Enterobacter sakazakii has emerged as a rare cause of neonatal meningitis, septicemia and enterocolitis. Contaminated infant milk formula (IMF) has been identified as one infection route. A small number of clinical outbreaks have been epidemiologically linked to IMF contaminated post-pasteurization during manufacture and/or mishandled when reconstituted. Currently no agreed standardized typing protocol has been developed to trace
E. sakazakii. The objectives of this study were to apply biochemical and genetic methods to characterize 51 environmental and food
E. sakazakii isolates and 6
E. sakazakii type strains. Isolates were presumptively identified using biochemical profiles based on API 20E and ID32E methods and by culture on differential selective Druggan Forsythe Iversen (DFI) agar. Identification was subsequently confirmed by real time polymerase chain reaction (PCR).
All but one of the isolates was identified as
E. sakazakii by biochemical profiling. One isolate was identified as
Escherichia vulneris by ID 32E and as
Pantoea agglomerans by API 20E. All isolates produced green/blue colonies on DFI medium characteristic of this organism. Real time PCR could differentiate between
E. sakazakii, Enterobacter spp. and other
Enterobacteriacae. Analysis of RAPD banding patterns revealed 3 major clusters of
E. sakazakii. There was a large degree of diversity noted amongst the remaining isolates. Our findings indicate that RAPD may be applied as a useful and reliable tool for direct comparison of
E. sakazakii isolates providing traceability through the infant formula food chain.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16730386</pmid><doi>10.1016/j.ijfoodmicro.2006.02.008</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of food microbiology, 2006-07, Vol.110 (2), p.127-134 |
| issn | 0168-1605 1879-3460 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Cluster Analysis Colony Count, Microbial Cronobacter sakazakii - classification Cronobacter sakazakii - genetics Cronobacter sakazakii - isolation & purification DNA, Bacterial - analysis Enterobacter Enterobacter sakazakii Enterobacteriacae Enterobacteriaceae Environmental Microbiology Escherichia Food Contamination - analysis Food industries Food Microbiology Fundamental and applied biological sciences. Psychology Gene Expression Profiling Humans Infant Infant Food - microbiology Infant, Newborn Neonatal meningitis Oligonucleotide Array Sequence Analysis Pantoea agglomerans Phylogeny Polymerase Chain Reaction Random Amplified Polymorphic DNA Technique RAPD typing Sensitivity and Specificity Species Specificity |
| title | Characterization of a collection of Enterobacter sakazakii isolates from environmental and food sources |
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