Enzymatic Synthesis of Bis(5′-Nucleosidyl) Tetra- and Triphosphates

Enzymatic Synthesis of Bis(5′-Nucleosidyl) Tetra- and Triphosphates

The total fraction of aminoacyl-tRNA synthases from Escherichia coli has been shown to catalyze the synthesis of the bis(5'-nucleosidyl) oligophosphates Ap sub(4)AZT, Ap sub(4)d4T, Ap sub(4)3TC, and Ap sub(4)ACV, as well as Ap sub(3)AZT and Ap sub(3)d4T, from [ alpha - super(32)P]ATP and the co...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Russian journal of bioorganic chemistry 2005-11, Vol.31 (6), p.563-566
Hauptverfasser: Skoblov, A. Yu, Yas'ko, M. V., Murabuldaev, A. M., Kukhanova, M. K., Skoblov, Yu. S.
Format: Artikel
Sprache:eng
Schlagworte:
Escherichia coli
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 566
container_issue 6
container_start_page 563
container_title Russian journal of bioorganic chemistry
container_volume 31
creator Skoblov, A. Yu
Yas'ko, M. V.
Murabuldaev, A. M.
Kukhanova, M. K.
Skoblov, Yu. S.
description The total fraction of aminoacyl-tRNA synthases from Escherichia coli has been shown to catalyze the synthesis of the bis(5'-nucleosidyl) oligophosphates Ap sub(4)AZT, Ap sub(4)d4T, Ap sub(4)3TC, and Ap sub(4)ACV, as well as Ap sub(3)AZT and Ap sub(3)d4T, from [ alpha - super(32)P]ATP and the corresponding nucleoside-5'-tri(or di)phosphate. The resulting compounds, characterized by HPLC, are resistant to alkaline phosphatase. Ap sub(4)AZT, Ap sub(4)d4T, and Ap sub(4)3TC are formed with approximately equal efficiency, whereas the efficiencies of the synthesis of Ap sub(4)ACV, Ap sub(3)AZT, and Ap sub(3)d4T are three- to fivefold lower.
doi_str_mv 10.1007/s11171-005-0077-6
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19772997</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>19772997</sourcerecordid><originalsourceid>FETCH-LOGICAL-c228t-10e118987564d4b5e50a1c38f3718a1a2b289a17235ab433f7af1e601c71fbc43</originalsourceid><addsrcrecordid>eNotkL1OwzAcxC0EEqXwAGyZEAwG_-34IyNU5UOqYKDMluM6qlGaBP_TIUx9Jh6JJyFVGU53w90NP0Iugd0CY_oOAUADZUyO0pqqIzIBqQUtQInjMTNlKCjOTskZ4idj40qaCZnPm-9h4_ros_eh6dcBI2ZtlT1EvJa_ux_6uvV1aDGuhvomW4Y-OZq5ZpUtU-zWLXZr1wc8JyeVqzFc_PuUfDzOl7Nnunh7epndL6jn3PQUWAAwhdFS5au8lEEyB16YSmgwDhwvuSkcaC6kK3MhKu0qCIqB11CVPhdTcnX47VL7tQ3Y201EH-raNaHdooVCa14UeizCoehTi5hCZbsUNy4NFpjdA7MHYHakYPfArBJ_g9hd4A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19772997</pqid></control><display><type>article</type><title>Enzymatic Synthesis of Bis(5′-Nucleosidyl) Tetra- and Triphosphates</title><source>Springer Journals</source><creator>Skoblov, A. Yu ; Yas'ko, M. V. ; Murabuldaev, A. M. ; Kukhanova, M. K. ; Skoblov, Yu. S.</creator><creatorcontrib>Skoblov, A. Yu ; Yas'ko, M. V. ; Murabuldaev, A. M. ; Kukhanova, M. K. ; Skoblov, Yu. S.</creatorcontrib><description>The total fraction of aminoacyl-tRNA synthases from Escherichia coli has been shown to catalyze the synthesis of the bis(5'-nucleosidyl) oligophosphates Ap sub(4)AZT, Ap sub(4)d4T, Ap sub(4)3TC, and Ap sub(4)ACV, as well as Ap sub(3)AZT and Ap sub(3)d4T, from [ alpha - super(32)P]ATP and the corresponding nucleoside-5'-tri(or di)phosphate. The resulting compounds, characterized by HPLC, are resistant to alkaline phosphatase. Ap sub(4)AZT, Ap sub(4)d4T, and Ap sub(4)3TC are formed with approximately equal efficiency, whereas the efficiencies of the synthesis of Ap sub(4)ACV, Ap sub(3)AZT, and Ap sub(3)d4T are three- to fivefold lower.</description><identifier>ISSN: 1068-1620</identifier><identifier>EISSN: 1573-9163</identifier><identifier>EISSN: 1608-330X</identifier><identifier>DOI: 10.1007/s11171-005-0077-6</identifier><language>eng</language><subject>Escherichia coli</subject><ispartof>Russian journal of bioorganic chemistry, 2005-11, Vol.31 (6), p.563-566</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c228t-10e118987564d4b5e50a1c38f3718a1a2b289a17235ab433f7af1e601c71fbc43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Skoblov, A. Yu</creatorcontrib><creatorcontrib>Yas'ko, M. V.</creatorcontrib><creatorcontrib>Murabuldaev, A. M.</creatorcontrib><creatorcontrib>Kukhanova, M. K.</creatorcontrib><creatorcontrib>Skoblov, Yu. S.</creatorcontrib><title>Enzymatic Synthesis of Bis(5′-Nucleosidyl) Tetra- and Triphosphates</title><title>Russian journal of bioorganic chemistry</title><description>The total fraction of aminoacyl-tRNA synthases from Escherichia coli has been shown to catalyze the synthesis of the bis(5'-nucleosidyl) oligophosphates Ap sub(4)AZT, Ap sub(4)d4T, Ap sub(4)3TC, and Ap sub(4)ACV, as well as Ap sub(3)AZT and Ap sub(3)d4T, from [ alpha - super(32)P]ATP and the corresponding nucleoside-5'-tri(or di)phosphate. The resulting compounds, characterized by HPLC, are resistant to alkaline phosphatase. Ap sub(4)AZT, Ap sub(4)d4T, and Ap sub(4)3TC are formed with approximately equal efficiency, whereas the efficiencies of the synthesis of Ap sub(4)ACV, Ap sub(3)AZT, and Ap sub(3)d4T are three- to fivefold lower.</description><subject>Escherichia coli</subject><issn>1068-1620</issn><issn>1573-9163</issn><issn>1608-330X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNotkL1OwzAcxC0EEqXwAGyZEAwG_-34IyNU5UOqYKDMluM6qlGaBP_TIUx9Jh6JJyFVGU53w90NP0Iugd0CY_oOAUADZUyO0pqqIzIBqQUtQInjMTNlKCjOTskZ4idj40qaCZnPm-9h4_ros_eh6dcBI2ZtlT1EvJa_ux_6uvV1aDGuhvomW4Y-OZq5ZpUtU-zWLXZr1wc8JyeVqzFc_PuUfDzOl7Nnunh7epndL6jn3PQUWAAwhdFS5au8lEEyB16YSmgwDhwvuSkcaC6kK3MhKu0qCIqB11CVPhdTcnX47VL7tQ3Y201EH-raNaHdooVCa14UeizCoehTi5hCZbsUNy4NFpjdA7MHYHakYPfArBJ_g9hd4A</recordid><startdate>200511</startdate><enddate>200511</enddate><creator>Skoblov, A. Yu</creator><creator>Yas'ko, M. V.</creator><creator>Murabuldaev, A. M.</creator><creator>Kukhanova, M. K.</creator><creator>Skoblov, Yu. S.</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>200511</creationdate><title>Enzymatic Synthesis of Bis(5′-Nucleosidyl) Tetra- and Triphosphates</title><author>Skoblov, A. Yu ; Yas'ko, M. V. ; Murabuldaev, A. M. ; Kukhanova, M. K. ; Skoblov, Yu. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c228t-10e118987564d4b5e50a1c38f3718a1a2b289a17235ab433f7af1e601c71fbc43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Escherichia coli</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Skoblov, A. Yu</creatorcontrib><creatorcontrib>Yas'ko, M. V.</creatorcontrib><creatorcontrib>Murabuldaev, A. M.</creatorcontrib><creatorcontrib>Kukhanova, M. K.</creatorcontrib><creatorcontrib>Skoblov, Yu. S.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Russian journal of bioorganic chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Skoblov, A. Yu</au><au>Yas'ko, M. V.</au><au>Murabuldaev, A. M.</au><au>Kukhanova, M. K.</au><au>Skoblov, Yu. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic Synthesis of Bis(5′-Nucleosidyl) Tetra- and Triphosphates</atitle><jtitle>Russian journal of bioorganic chemistry</jtitle><date>2005-11</date><risdate>2005</risdate><volume>31</volume><issue>6</issue><spage>563</spage><epage>566</epage><pages>563-566</pages><issn>1068-1620</issn><eissn>1573-9163</eissn><eissn>1608-330X</eissn><abstract>The total fraction of aminoacyl-tRNA synthases from Escherichia coli has been shown to catalyze the synthesis of the bis(5'-nucleosidyl) oligophosphates Ap sub(4)AZT, Ap sub(4)d4T, Ap sub(4)3TC, and Ap sub(4)ACV, as well as Ap sub(3)AZT and Ap sub(3)d4T, from [ alpha - super(32)P]ATP and the corresponding nucleoside-5'-tri(or di)phosphate. The resulting compounds, characterized by HPLC, are resistant to alkaline phosphatase. Ap sub(4)AZT, Ap sub(4)d4T, and Ap sub(4)3TC are formed with approximately equal efficiency, whereas the efficiencies of the synthesis of Ap sub(4)ACV, Ap sub(3)AZT, and Ap sub(3)d4T are three- to fivefold lower.</abstract><doi>10.1007/s11171-005-0077-6</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1068-1620
ispartof Russian journal of bioorganic chemistry, 2005-11, Vol.31 (6), p.563-566
issn 1068-1620
1573-9163
1608-330X
language eng
recordid cdi_proquest_miscellaneous_19772997
source Springer Journals
subjects Escherichia coli
title Enzymatic Synthesis of Bis(5′-Nucleosidyl) Tetra- and Triphosphates
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T18%3A13%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enzymatic%20Synthesis%20of%20Bis(5%E2%80%B2-Nucleosidyl)%20Tetra-%20and%20Triphosphates&rft.jtitle=Russian%20journal%20of%20bioorganic%20chemistry&rft.au=Skoblov,%20A.%20Yu&rft.date=2005-11&rft.volume=31&rft.issue=6&rft.spage=563&rft.epage=566&rft.pages=563-566&rft.issn=1068-1620&rft.eissn=1573-9163&rft_id=info:doi/10.1007/s11171-005-0077-6&rft_dat=%3Cproquest_cross%3E19772997%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19772997&rft_id=info:pmid/&rfr_iscdi=true