Laboratory-evolved vanadium chloroperoxidase exhibits 100-fold higher halogenating activity at alkaline pH: catalytic effects from first and second coordination sphere mutations

Directed evolution was performed on vanadium chloroperoxidase from the fungus Curvularia inaequalis to increase its brominating activity at a mildly alkaline pH for industrial and synthetic applications and to further understand its mechanism. After successful expression of the enzyme in Escherichia...

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Veröffentlicht in:	The Journal of biological chemistry 2006-04, Vol.281 (14), p.9738-9744
Hauptverfasser:	Hasan, Zulfiqar, Renirie, Rokus, Kerkman, Richard, Ruijssenaars, Harald J, Hartog, Aloysius F, Wever, Ron
Format:	Artikel
Sprache:	eng
Schlagworte:	Ascomycota - enzymology Ascophyllum - enzymology Ascophyllum nodosum Bromine - metabolism Catalysis Chloride Peroxidase - genetics Chloride Peroxidase - metabolism Curvularia inaequalis Directed Molecular Evolution Escherichia coli Escherichia coli - genetics Halogens - metabolism Hydrogen-Ion Concentration Oxidation-Reduction
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