BM-derived cells restore expression of peroxiredoxin V in the airways following acute naphthalene injury in mice
Naphthalene-induced respiratory tract toxicity in mice is characterized by specific and rapid loss of the Clara cell population, which is restored only after several days. The sources of restoration of this cell population remain unclear. We investigated whether BM-derived cells participated in the...
Gespeichert in:
| Veröffentlicht in: | Cytotherapy (Oxford, England) England), 2005, Vol.7 (6), p.483-493 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 493 |
|---|---|
| container_issue | 6 |
| container_start_page | 483 |
| container_title | Cytotherapy (Oxford, England) |
| container_volume | 7 |
| creator | Serikov, V.B. Popov, B.V. Kropotov, A.V. Tomilin, N.V. |
| description | Naphthalene-induced respiratory tract toxicity in mice is characterized by specific and rapid loss of the Clara cell population, which is restored only after several days. The sources of restoration of this cell population remain unclear. We investigated whether BM-derived cells participated in the process of epithelial restoration following naphthalene toxicity compared with bacterial infection. We further investigated the role of BM-derived cells in restoration of expression of peroxiredoxin V (PRXV), one of the major proteins of antioxidant defense, specifically expressed in the bronchial epithelium.
We transplanted GFP-tagged BM cells into 5 Gy-irradiated C57BL/6 recipients. Following 1 month of recovery, experimental animals were subjected to 250mg/kg naphthalene i.p. An additional group of animals received intratracheal instillation of Escherichia coli to induce acute bacterial inflammation. Animals were killed at 1–12 days after naphthalene and analyzed immunohistochemically.
Recipients’ cells of bronchial epithelium demonstrated significantly reduced levels of PRXV expression following naphthalene. In animals with acute bacterial inflammation, PRXV levels were not reduced in epithelium and participation of BM-derived cells in epithelial restoration was minimal. Following naphthalene, GFP+ cells were present in large numbers in lung parenchyma and epithelium of conducting airways starting at 1 day following injury. GFP+ progeny of BM cells was the major source of PRXV in the epithelium.
These data suggest that BM-derived cells may provide a source of antioxidant protection of airways by expression of PRXV in a model of acute epithelial respiratory tract toxicity. |
| doi_str_mv | 10.1080/14653240500361012 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19771286</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1465324905708380</els_id><sourcerecordid>19771286</sourcerecordid><originalsourceid>FETCH-LOGICAL-c434t-43c302040f35865e5de4e7aa52999e02413e7764a1c744f2801821529ba6025d3</originalsourceid><addsrcrecordid>eNp9kUtv1DAUha0KRB_wA9ggr9gFrp9J1BWtgCIVsaHdWq5z03iU2KmdtJ1_j0czEotK3Vxfyd858jkm5CODLwwa-MqkVoJLUABCM2D8iJwwWdcVV1q_2e1aVQVoj8lpzhsADk2j3pFjpgVoYHBC5ovfVYfJP2JHHY5jpgnzEhNSfJ7Lmn0MNPZ0xhSffcKuzEBvaRnLgNT69GS3mfZxHOOTD_fUunVBGuw8LIMdMWBBN2va7hSTd_ievO3tmPHD4TwjNz--_728qq7__Px1-e26clLIpZLCifJcCb1QjVaoOpRYW6t427YIXDKBda2lZa6WsucNsIazcntnNXDViTPyee87p_iwlkxm8nmX0AaMazasrWvGG11Atgddijkn7M2c_GTT1jAwu5rNi5qL5tPBfL2bsPuvOPRagPM94EMf02QHtOMyOJvQbOKaQkn-qv1BjaWgR4_JZOcxOOzKD7jFdNG_ov4HqNGcYQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19771286</pqid></control><display><type>article</type><title>BM-derived cells restore expression of peroxiredoxin V in the airways following acute naphthalene injury in mice</title><source>MEDLINE</source><source>Access via Taylor & Francis</source><source>Alma/SFX Local Collection</source><creator>Serikov, V.B. ; Popov, B.V. ; Kropotov, A.V. ; Tomilin, N.V.</creator><creatorcontrib>Serikov, V.B. ; Popov, B.V. ; Kropotov, A.V. ; Tomilin, N.V.</creatorcontrib><description>Naphthalene-induced respiratory tract toxicity in mice is characterized by specific and rapid loss of the Clara cell population, which is restored only after several days. The sources of restoration of this cell population remain unclear. We investigated whether BM-derived cells participated in the process of epithelial restoration following naphthalene toxicity compared with bacterial infection. We further investigated the role of BM-derived cells in restoration of expression of peroxiredoxin V (PRXV), one of the major proteins of antioxidant defense, specifically expressed in the bronchial epithelium.
We transplanted GFP-tagged BM cells into 5 Gy-irradiated C57BL/6 recipients. Following 1 month of recovery, experimental animals were subjected to 250mg/kg naphthalene i.p. An additional group of animals received intratracheal instillation of Escherichia coli to induce acute bacterial inflammation. Animals were killed at 1–12 days after naphthalene and analyzed immunohistochemically.
Recipients’ cells of bronchial epithelium demonstrated significantly reduced levels of PRXV expression following naphthalene. In animals with acute bacterial inflammation, PRXV levels were not reduced in epithelium and participation of BM-derived cells in epithelial restoration was minimal. Following naphthalene, GFP+ cells were present in large numbers in lung parenchyma and epithelium of conducting airways starting at 1 day following injury. GFP+ progeny of BM cells was the major source of PRXV in the epithelium.
These data suggest that BM-derived cells may provide a source of antioxidant protection of airways by expression of PRXV in a model of acute epithelial respiratory tract toxicity.</description><identifier>ISSN: 1465-3249</identifier><identifier>EISSN: 1477-2566</identifier><identifier>DOI: 10.1080/14653240500361012</identifier><identifier>PMID: 16306010</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Animals ; Bone Marrow Cells - metabolism ; Bone Marrow Transplantation ; Bronchi - drug effects ; Bronchi - metabolism ; Bronchi - pathology ; Epithelium - drug effects ; Epithelium - metabolism ; Epithelium - pathology ; Escherichia coli ; Escherichia coli K12 ; Female ; Green Fluorescent Proteins ; lung ; Lung - drug effects ; Lung - metabolism ; Lung - pathology ; Male ; Mice ; Mice, Inbred C57BL ; Microscopy, Confocal ; naphthalene ; Naphthalenes ; Peroxidases - biosynthesis ; Peroxidases - metabolism ; peroxiredoxin ; Peroxiredoxins ; stem cells ; Time Factors ; Whole-Body Irradiation</subject><ispartof>Cytotherapy (Oxford, England), 2005, Vol.7 (6), p.483-493</ispartof><rights>2005 International Society for Cellular Therapy</rights><rights>2005 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-43c302040f35865e5de4e7aa52999e02413e7764a1c744f2801821529ba6025d3</citedby><cites>FETCH-LOGICAL-c434t-43c302040f35865e5de4e7aa52999e02413e7764a1c744f2801821529ba6025d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/14653240500361012$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/14653240500361012$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,61221,61402</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16306010$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Serikov, V.B.</creatorcontrib><creatorcontrib>Popov, B.V.</creatorcontrib><creatorcontrib>Kropotov, A.V.</creatorcontrib><creatorcontrib>Tomilin, N.V.</creatorcontrib><title>BM-derived cells restore expression of peroxiredoxin V in the airways following acute naphthalene injury in mice</title><title>Cytotherapy (Oxford, England)</title><addtitle>Cytotherapy</addtitle><description>Naphthalene-induced respiratory tract toxicity in mice is characterized by specific and rapid loss of the Clara cell population, which is restored only after several days. The sources of restoration of this cell population remain unclear. We investigated whether BM-derived cells participated in the process of epithelial restoration following naphthalene toxicity compared with bacterial infection. We further investigated the role of BM-derived cells in restoration of expression of peroxiredoxin V (PRXV), one of the major proteins of antioxidant defense, specifically expressed in the bronchial epithelium.
We transplanted GFP-tagged BM cells into 5 Gy-irradiated C57BL/6 recipients. Following 1 month of recovery, experimental animals were subjected to 250mg/kg naphthalene i.p. An additional group of animals received intratracheal instillation of Escherichia coli to induce acute bacterial inflammation. Animals were killed at 1–12 days after naphthalene and analyzed immunohistochemically.
Recipients’ cells of bronchial epithelium demonstrated significantly reduced levels of PRXV expression following naphthalene. In animals with acute bacterial inflammation, PRXV levels were not reduced in epithelium and participation of BM-derived cells in epithelial restoration was minimal. Following naphthalene, GFP+ cells were present in large numbers in lung parenchyma and epithelium of conducting airways starting at 1 day following injury. GFP+ progeny of BM cells was the major source of PRXV in the epithelium.
These data suggest that BM-derived cells may provide a source of antioxidant protection of airways by expression of PRXV in a model of acute epithelial respiratory tract toxicity.</description><subject>Animals</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Bone Marrow Transplantation</subject><subject>Bronchi - drug effects</subject><subject>Bronchi - metabolism</subject><subject>Bronchi - pathology</subject><subject>Epithelium - drug effects</subject><subject>Epithelium - metabolism</subject><subject>Epithelium - pathology</subject><subject>Escherichia coli</subject><subject>Escherichia coli K12</subject><subject>Female</subject><subject>Green Fluorescent Proteins</subject><subject>lung</subject><subject>Lung - drug effects</subject><subject>Lung - metabolism</subject><subject>Lung - pathology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microscopy, Confocal</subject><subject>naphthalene</subject><subject>Naphthalenes</subject><subject>Peroxidases - biosynthesis</subject><subject>Peroxidases - metabolism</subject><subject>peroxiredoxin</subject><subject>Peroxiredoxins</subject><subject>stem cells</subject><subject>Time Factors</subject><subject>Whole-Body Irradiation</subject><issn>1465-3249</issn><issn>1477-2566</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAUha0KRB_wA9ggr9gFrp9J1BWtgCIVsaHdWq5z03iU2KmdtJ1_j0czEotK3Vxfyd858jkm5CODLwwa-MqkVoJLUABCM2D8iJwwWdcVV1q_2e1aVQVoj8lpzhsADk2j3pFjpgVoYHBC5ovfVYfJP2JHHY5jpgnzEhNSfJ7Lmn0MNPZ0xhSffcKuzEBvaRnLgNT69GS3mfZxHOOTD_fUunVBGuw8LIMdMWBBN2va7hSTd_ievO3tmPHD4TwjNz--_728qq7__Px1-e26clLIpZLCifJcCb1QjVaoOpRYW6t427YIXDKBda2lZa6WsucNsIazcntnNXDViTPyee87p_iwlkxm8nmX0AaMazasrWvGG11Atgddijkn7M2c_GTT1jAwu5rNi5qL5tPBfL2bsPuvOPRagPM94EMf02QHtOMyOJvQbOKaQkn-qv1BjaWgR4_JZOcxOOzKD7jFdNG_ov4HqNGcYQ</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>Serikov, V.B.</creator><creator>Popov, B.V.</creator><creator>Kropotov, A.V.</creator><creator>Tomilin, N.V.</creator><general>Elsevier Inc</general><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>2005</creationdate><title>BM-derived cells restore expression of peroxiredoxin V in the airways following acute naphthalene injury in mice</title><author>Serikov, V.B. ; Popov, B.V. ; Kropotov, A.V. ; Tomilin, N.V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-43c302040f35865e5de4e7aa52999e02413e7764a1c744f2801821529ba6025d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Bone Marrow Transplantation</topic><topic>Bronchi - drug effects</topic><topic>Bronchi - metabolism</topic><topic>Bronchi - pathology</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - metabolism</topic><topic>Epithelium - pathology</topic><topic>Escherichia coli</topic><topic>Escherichia coli K12</topic><topic>Female</topic><topic>Green Fluorescent Proteins</topic><topic>lung</topic><topic>Lung - drug effects</topic><topic>Lung - metabolism</topic><topic>Lung - pathology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microscopy, Confocal</topic><topic>naphthalene</topic><topic>Naphthalenes</topic><topic>Peroxidases - biosynthesis</topic><topic>Peroxidases - metabolism</topic><topic>peroxiredoxin</topic><topic>Peroxiredoxins</topic><topic>stem cells</topic><topic>Time Factors</topic><topic>Whole-Body Irradiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Serikov, V.B.</creatorcontrib><creatorcontrib>Popov, B.V.</creatorcontrib><creatorcontrib>Kropotov, A.V.</creatorcontrib><creatorcontrib>Tomilin, N.V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Cytotherapy (Oxford, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Serikov, V.B.</au><au>Popov, B.V.</au><au>Kropotov, A.V.</au><au>Tomilin, N.V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>BM-derived cells restore expression of peroxiredoxin V in the airways following acute naphthalene injury in mice</atitle><jtitle>Cytotherapy (Oxford, England)</jtitle><addtitle>Cytotherapy</addtitle><date>2005</date><risdate>2005</risdate><volume>7</volume><issue>6</issue><spage>483</spage><epage>493</epage><pages>483-493</pages><issn>1465-3249</issn><eissn>1477-2566</eissn><abstract>Naphthalene-induced respiratory tract toxicity in mice is characterized by specific and rapid loss of the Clara cell population, which is restored only after several days. The sources of restoration of this cell population remain unclear. We investigated whether BM-derived cells participated in the process of epithelial restoration following naphthalene toxicity compared with bacterial infection. We further investigated the role of BM-derived cells in restoration of expression of peroxiredoxin V (PRXV), one of the major proteins of antioxidant defense, specifically expressed in the bronchial epithelium.
We transplanted GFP-tagged BM cells into 5 Gy-irradiated C57BL/6 recipients. Following 1 month of recovery, experimental animals were subjected to 250mg/kg naphthalene i.p. An additional group of animals received intratracheal instillation of Escherichia coli to induce acute bacterial inflammation. Animals were killed at 1–12 days after naphthalene and analyzed immunohistochemically.
Recipients’ cells of bronchial epithelium demonstrated significantly reduced levels of PRXV expression following naphthalene. In animals with acute bacterial inflammation, PRXV levels were not reduced in epithelium and participation of BM-derived cells in epithelial restoration was minimal. Following naphthalene, GFP+ cells were present in large numbers in lung parenchyma and epithelium of conducting airways starting at 1 day following injury. GFP+ progeny of BM cells was the major source of PRXV in the epithelium.
These data suggest that BM-derived cells may provide a source of antioxidant protection of airways by expression of PRXV in a model of acute epithelial respiratory tract toxicity.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>16306010</pmid><doi>10.1080/14653240500361012</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1465-3249 |
| ispartof | Cytotherapy (Oxford, England), 2005, Vol.7 (6), p.483-493 |
| issn | 1465-3249 1477-2566 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19771286 |
| source | MEDLINE; Access via Taylor & Francis; Alma/SFX Local Collection |
| subjects | Animals Bone Marrow Cells - metabolism Bone Marrow Transplantation Bronchi - drug effects Bronchi - metabolism Bronchi - pathology Epithelium - drug effects Epithelium - metabolism Epithelium - pathology Escherichia coli Escherichia coli K12 Female Green Fluorescent Proteins lung Lung - drug effects Lung - metabolism Lung - pathology Male Mice Mice, Inbred C57BL Microscopy, Confocal naphthalene Naphthalenes Peroxidases - biosynthesis Peroxidases - metabolism peroxiredoxin Peroxiredoxins stem cells Time Factors Whole-Body Irradiation |
| title | BM-derived cells restore expression of peroxiredoxin V in the airways following acute naphthalene injury in mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T17%3A21%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=BM-derived%20cells%20restore%20expression%20of%20peroxiredoxin%20V%20in%20the%20airways%20following%20acute%20naphthalene%20injury%20in%20mice&rft.jtitle=Cytotherapy%20(Oxford,%20England)&rft.au=Serikov,%20V.B.&rft.date=2005&rft.volume=7&rft.issue=6&rft.spage=483&rft.epage=493&rft.pages=483-493&rft.issn=1465-3249&rft.eissn=1477-2566&rft_id=info:doi/10.1080/14653240500361012&rft_dat=%3Cproquest_cross%3E19771286%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19771286&rft_id=info:pmid/16306010&rft_els_id=S1465324905708380&rfr_iscdi=true |