Microbial degradation of pyridine using Pseudomonas sp. and isolation of plasmid responsible for degradation

Pseudomonas (PI2) capable of degrading pyridine was isolated from the mixed population of the activated sludge unit which was being used for treating complex effluents, the strain was characterized. Aerobic degradation of pyridine was studied with the isolated strain and the growth parameters were e...

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Veröffentlicht in:Waste management (Elmsford) 2003, Vol.23 (2), p.167-171
Hauptverfasser: Mohan, S.Venkata, Sistla, Srinivas, Guru, R.Kumar, Prasad, K.Krishna, Kumar, C.Suresh, Ramakrishna, S.V., Sarma, P.N.
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container_end_page 171
container_issue 2
container_start_page 167
container_title Waste management (Elmsford)
container_volume 23
creator Mohan, S.Venkata
Sistla, Srinivas
Guru, R.Kumar
Prasad, K.Krishna
Kumar, C.Suresh
Ramakrishna, S.V.
Sarma, P.N.
description Pseudomonas (PI2) capable of degrading pyridine was isolated from the mixed population of the activated sludge unit which was being used for treating complex effluents, the strain was characterized. Aerobic degradation of pyridine was studied with the isolated strain and the growth parameters were evaluated. Pyridine degradation was further conformed by chromatography (HPLC) analysis. The process parameters like biomass growth and dissolved oxygen consumption were monitored during pyridine degradation. In order to conform with the plasmid capability to degrade pyridine, the requisite plasmid was isolated and transferred to DH 5α Escherichia coli. The subsequent biodegradation studies revealed the ability of the transformed plasmid capability to degrade the pyridine.
doi_str_mv 10.1016/S0956-053X(02)00150-2
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subjects Activated sludge
Applied sciences
Biodegradation, Environmental
Biological and medical sciences
Biological treatment of waters
Biomass
Biotechnology
Chromatography, High Pressure Liquid
Environment and pollution
Escherichia coli
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
Industrial wastewaters
Oxygen Consumption
Plasmids - isolation & purification
Plasmids - physiology
Pollution
Pseudomonas - physiology
pyridine
Pyridines - metabolism
Sewage - chemistry
Sewage - microbiology
Wastewaters
Water treatment and pollution
title Microbial degradation of pyridine using Pseudomonas sp. and isolation of plasmid responsible for degradation
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