Microbial degradation of pyridine using Pseudomonas sp. and isolation of plasmid responsible for degradation
Pseudomonas (PI2) capable of degrading pyridine was isolated from the mixed population of the activated sludge unit which was being used for treating complex effluents, the strain was characterized. Aerobic degradation of pyridine was studied with the isolated strain and the growth parameters were e...
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Veröffentlicht in: | Waste management (Elmsford) 2003, Vol.23 (2), p.167-171 |
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creator | Mohan, S.Venkata Sistla, Srinivas Guru, R.Kumar Prasad, K.Krishna Kumar, C.Suresh Ramakrishna, S.V. Sarma, P.N. |
description | Pseudomonas (PI2) capable of degrading pyridine was isolated from the mixed population of the activated sludge unit which was being used for treating complex effluents, the strain was characterized. Aerobic degradation of pyridine was studied with the isolated strain and the growth parameters were evaluated. Pyridine degradation was further conformed by chromatography (HPLC) analysis. The process parameters like biomass growth and dissolved oxygen consumption were monitored during pyridine degradation. In order to conform with the plasmid capability to degrade pyridine, the requisite plasmid was isolated and transferred to DH 5α
Escherichia coli. The subsequent biodegradation studies revealed the ability of the transformed plasmid capability to degrade the pyridine. |
doi_str_mv | 10.1016/S0956-053X(02)00150-2 |
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Escherichia coli. The subsequent biodegradation studies revealed the ability of the transformed plasmid capability to degrade the pyridine.</description><identifier>ISSN: 0956-053X</identifier><identifier>EISSN: 1879-2456</identifier><identifier>DOI: 10.1016/S0956-053X(02)00150-2</identifier><identifier>PMID: 12623091</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Activated sludge ; Applied sciences ; Biodegradation, Environmental ; Biological and medical sciences ; Biological treatment of waters ; Biomass ; Biotechnology ; Chromatography, High Pressure Liquid ; Environment and pollution ; Escherichia coli ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Industrial applications and implications. Economical aspects ; Industrial wastewaters ; Oxygen Consumption ; Plasmids - isolation & purification ; Plasmids - physiology ; Pollution ; Pseudomonas - physiology ; pyridine ; Pyridines - metabolism ; Sewage - chemistry ; Sewage - microbiology ; Wastewaters ; Water treatment and pollution</subject><ispartof>Waste management (Elmsford), 2003, Vol.23 (2), p.167-171</ispartof><rights>2003 Elsevier Science Ltd</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-96019385bfe69246584245c9d77498cf49dbcc413dfa19ee748add895584db823</citedby><cites>FETCH-LOGICAL-c474t-96019385bfe69246584245c9d77498cf49dbcc413dfa19ee748add895584db823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956053X02001502$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14570886$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12623091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mohan, S.Venkata</creatorcontrib><creatorcontrib>Sistla, Srinivas</creatorcontrib><creatorcontrib>Guru, R.Kumar</creatorcontrib><creatorcontrib>Prasad, K.Krishna</creatorcontrib><creatorcontrib>Kumar, C.Suresh</creatorcontrib><creatorcontrib>Ramakrishna, S.V.</creatorcontrib><creatorcontrib>Sarma, P.N.</creatorcontrib><title>Microbial degradation of pyridine using Pseudomonas sp. and isolation of plasmid responsible for degradation</title><title>Waste management (Elmsford)</title><addtitle>Waste Manag</addtitle><description>Pseudomonas (PI2) capable of degrading pyridine was isolated from the mixed population of the activated sludge unit which was being used for treating complex effluents, the strain was characterized. Aerobic degradation of pyridine was studied with the isolated strain and the growth parameters were evaluated. Pyridine degradation was further conformed by chromatography (HPLC) analysis. The process parameters like biomass growth and dissolved oxygen consumption were monitored during pyridine degradation. In order to conform with the plasmid capability to degrade pyridine, the requisite plasmid was isolated and transferred to DH 5α
Escherichia coli. The subsequent biodegradation studies revealed the ability of the transformed plasmid capability to degrade the pyridine.</description><subject>Activated sludge</subject><subject>Applied sciences</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Biological treatment of waters</subject><subject>Biomass</subject><subject>Biotechnology</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Environment and pollution</subject><subject>Escherichia coli</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Industrial applications and implications. 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Aerobic degradation of pyridine was studied with the isolated strain and the growth parameters were evaluated. Pyridine degradation was further conformed by chromatography (HPLC) analysis. The process parameters like biomass growth and dissolved oxygen consumption were monitored during pyridine degradation. In order to conform with the plasmid capability to degrade pyridine, the requisite plasmid was isolated and transferred to DH 5α
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subjects | Activated sludge Applied sciences Biodegradation, Environmental Biological and medical sciences Biological treatment of waters Biomass Biotechnology Chromatography, High Pressure Liquid Environment and pollution Escherichia coli Exact sciences and technology Fundamental and applied biological sciences. Psychology Industrial applications and implications. Economical aspects Industrial wastewaters Oxygen Consumption Plasmids - isolation & purification Plasmids - physiology Pollution Pseudomonas - physiology pyridine Pyridines - metabolism Sewage - chemistry Sewage - microbiology Wastewaters Water treatment and pollution |
title | Microbial degradation of pyridine using Pseudomonas sp. and isolation of plasmid responsible for degradation |
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