Effect of age and body mass index on the yield of stromal vascular fraction
Summary Objective The aim of this study was to determine the influence of age, body mass index, and site of liposuction on the cell yield of SVF. Methods A prospective study was performed on 58 patients. The average age was 39 years old, with BMI ≤ 25 or BMI ≥ 25. Fat tissue was harvested from the a...
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| Veröffentlicht in: | Journal of cosmetic dermatology 2018-12, Vol.17 (6), p.1233-1239 |
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| container_issue | 6 |
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| container_title | Journal of cosmetic dermatology |
| container_volume | 17 |
| creator | Alaaeddine, Nada El Atat, Oula Saliba, Nagib Feghali, Ziad Nasr, Marwan Tarabey, Bilal Hilal, George Hashim, Hussein |
| description | Summary
Objective
The aim of this study was to determine the influence of age, body mass index, and site of liposuction on the cell yield of SVF.
Methods
A prospective study was performed on 58 patients. The average age was 39 years old, with BMI ≤ 25 or BMI ≥ 25. Fat tissue was harvested from the abdominal region, flanks, or thighs and SVF was isolated.
Results
The yield of viable SVF was evaluated by trypan blue, and the markers of stem cells were evaluated by flow cytometry. The cells were positive for stem cells markers, the age, sex of the patient had no impact on SVF cell yield with an average of 1.17 × 10^8. However, the BMI > 25 had resulted in higher cell numbers, and the harvest site had a significant impact on cell yield with abdomen being the site of interest.
Conclusion
These data demonstrate that the age of the person does not affect the cell yield of SVF; nevertheless, the donor site and BMI might be important factors in affecting cell number. |
| doi_str_mv | 10.1111/jocd.12458 |
| format | Article |
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Objective
The aim of this study was to determine the influence of age, body mass index, and site of liposuction on the cell yield of SVF.
Methods
A prospective study was performed on 58 patients. The average age was 39 years old, with BMI ≤ 25 or BMI ≥ 25. Fat tissue was harvested from the abdominal region, flanks, or thighs and SVF was isolated.
Results
The yield of viable SVF was evaluated by trypan blue, and the markers of stem cells were evaluated by flow cytometry. The cells were positive for stem cells markers, the age, sex of the patient had no impact on SVF cell yield with an average of 1.17 × 10^8. However, the BMI > 25 had resulted in higher cell numbers, and the harvest site had a significant impact on cell yield with abdomen being the site of interest.
Conclusion
These data demonstrate that the age of the person does not affect the cell yield of SVF; nevertheless, the donor site and BMI might be important factors in affecting cell number.</description><identifier>ISSN: 1473-2130</identifier><identifier>EISSN: 1473-2165</identifier><identifier>DOI: 10.1111/jocd.12458</identifier><identifier>PMID: 29222844</identifier><language>eng</language><publisher>England</publisher><subject>Abdomen ; Adipose Tissue - pathology ; adipose tissues ; Adult ; age ; Age Factors ; Aged ; BMI ; Body Mass Index ; Female ; harvest site ; Humans ; Lipectomy ; Male ; Middle Aged ; Pelvis ; Prospective Studies ; Sex Factors ; Stromal Cells ; stromal vascular fraction ; Thigh ; Young Adult</subject><ispartof>Journal of cosmetic dermatology, 2018-12, Vol.17 (6), p.1233-1239</ispartof><rights>2017 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3298-2754d74e40b9223c030f3b0de503a9e5cc66140a47af637fafeafb706c9a26be3</citedby><cites>FETCH-LOGICAL-c3298-2754d74e40b9223c030f3b0de503a9e5cc66140a47af637fafeafb706c9a26be3</cites><orcidid>0000-0002-1748-4473</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjocd.12458$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjocd.12458$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29222844$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alaaeddine, Nada</creatorcontrib><creatorcontrib>El Atat, Oula</creatorcontrib><creatorcontrib>Saliba, Nagib</creatorcontrib><creatorcontrib>Feghali, Ziad</creatorcontrib><creatorcontrib>Nasr, Marwan</creatorcontrib><creatorcontrib>Tarabey, Bilal</creatorcontrib><creatorcontrib>Hilal, George</creatorcontrib><creatorcontrib>Hashim, Hussein</creatorcontrib><title>Effect of age and body mass index on the yield of stromal vascular fraction</title><title>Journal of cosmetic dermatology</title><addtitle>J Cosmet Dermatol</addtitle><description>Summary
Objective
The aim of this study was to determine the influence of age, body mass index, and site of liposuction on the cell yield of SVF.
Methods
A prospective study was performed on 58 patients. The average age was 39 years old, with BMI ≤ 25 or BMI ≥ 25. Fat tissue was harvested from the abdominal region, flanks, or thighs and SVF was isolated.
Results
The yield of viable SVF was evaluated by trypan blue, and the markers of stem cells were evaluated by flow cytometry. The cells were positive for stem cells markers, the age, sex of the patient had no impact on SVF cell yield with an average of 1.17 × 10^8. However, the BMI > 25 had resulted in higher cell numbers, and the harvest site had a significant impact on cell yield with abdomen being the site of interest.
Conclusion
These data demonstrate that the age of the person does not affect the cell yield of SVF; nevertheless, the donor site and BMI might be important factors in affecting cell number.</description><subject>Abdomen</subject><subject>Adipose Tissue - pathology</subject><subject>adipose tissues</subject><subject>Adult</subject><subject>age</subject><subject>Age Factors</subject><subject>Aged</subject><subject>BMI</subject><subject>Body Mass Index</subject><subject>Female</subject><subject>harvest site</subject><subject>Humans</subject><subject>Lipectomy</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Pelvis</subject><subject>Prospective Studies</subject><subject>Sex Factors</subject><subject>Stromal Cells</subject><subject>stromal vascular fraction</subject><subject>Thigh</subject><subject>Young Adult</subject><issn>1473-2130</issn><issn>1473-2165</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQQC0EoqWw8AOQR4SU4q_EzYhK-azUBWbLsc-QKolLnAD596SkdOSWu-Hp6fQQOqdkSvu5Xntjp5SJeHaAxlRIHjGaxIf7m5MROglhTQiVKY2P0YiljLGZEGP0vHAOTIO9w_oNsK4szrztcKlDwHll4Rv7CjfvgLscCrvlQlP7Uhf4UwfTFrrGrtamyX11io6cLgKc7fYEvd4tXuYP0XJ1_zi_WUaGs3QWMRkLKwUIkvVvcEM4cTwjFmLCdQqxMUlCBdFCapdw6bQD7TJJEpNqlmTAJ-hy8G5q_9FCaFSZBwNFoSvwbVA0lb2Kp7Ho0asBNbUPoQanNnVe6rpTlKhtPLWNp37j9fDFzttmJdg9-lerB-gAfOUFdP-o1NNqfjtIfwCx2Hj0</recordid><startdate>201812</startdate><enddate>201812</enddate><creator>Alaaeddine, Nada</creator><creator>El Atat, Oula</creator><creator>Saliba, Nagib</creator><creator>Feghali, Ziad</creator><creator>Nasr, Marwan</creator><creator>Tarabey, Bilal</creator><creator>Hilal, George</creator><creator>Hashim, Hussein</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1748-4473</orcidid></search><sort><creationdate>201812</creationdate><title>Effect of age and body mass index on the yield of stromal vascular fraction</title><author>Alaaeddine, Nada ; El Atat, Oula ; Saliba, Nagib ; Feghali, Ziad ; Nasr, Marwan ; Tarabey, Bilal ; Hilal, George ; Hashim, Hussein</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3298-2754d74e40b9223c030f3b0de503a9e5cc66140a47af637fafeafb706c9a26be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Abdomen</topic><topic>Adipose Tissue - pathology</topic><topic>adipose tissues</topic><topic>Adult</topic><topic>age</topic><topic>Age Factors</topic><topic>Aged</topic><topic>BMI</topic><topic>Body Mass Index</topic><topic>Female</topic><topic>harvest site</topic><topic>Humans</topic><topic>Lipectomy</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Pelvis</topic><topic>Prospective Studies</topic><topic>Sex Factors</topic><topic>Stromal Cells</topic><topic>stromal vascular fraction</topic><topic>Thigh</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alaaeddine, Nada</creatorcontrib><creatorcontrib>El Atat, Oula</creatorcontrib><creatorcontrib>Saliba, Nagib</creatorcontrib><creatorcontrib>Feghali, Ziad</creatorcontrib><creatorcontrib>Nasr, Marwan</creatorcontrib><creatorcontrib>Tarabey, Bilal</creatorcontrib><creatorcontrib>Hilal, George</creatorcontrib><creatorcontrib>Hashim, Hussein</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cosmetic dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alaaeddine, Nada</au><au>El Atat, Oula</au><au>Saliba, Nagib</au><au>Feghali, Ziad</au><au>Nasr, Marwan</au><au>Tarabey, Bilal</au><au>Hilal, George</au><au>Hashim, Hussein</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of age and body mass index on the yield of stromal vascular fraction</atitle><jtitle>Journal of cosmetic dermatology</jtitle><addtitle>J Cosmet Dermatol</addtitle><date>2018-12</date><risdate>2018</risdate><volume>17</volume><issue>6</issue><spage>1233</spage><epage>1239</epage><pages>1233-1239</pages><issn>1473-2130</issn><eissn>1473-2165</eissn><abstract>Summary
Objective
The aim of this study was to determine the influence of age, body mass index, and site of liposuction on the cell yield of SVF.
Methods
A prospective study was performed on 58 patients. The average age was 39 years old, with BMI ≤ 25 or BMI ≥ 25. Fat tissue was harvested from the abdominal region, flanks, or thighs and SVF was isolated.
Results
The yield of viable SVF was evaluated by trypan blue, and the markers of stem cells were evaluated by flow cytometry. The cells were positive for stem cells markers, the age, sex of the patient had no impact on SVF cell yield with an average of 1.17 × 10^8. However, the BMI > 25 had resulted in higher cell numbers, and the harvest site had a significant impact on cell yield with abdomen being the site of interest.
Conclusion
These data demonstrate that the age of the person does not affect the cell yield of SVF; nevertheless, the donor site and BMI might be important factors in affecting cell number.</abstract><cop>England</cop><pmid>29222844</pmid><doi>10.1111/jocd.12458</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-1748-4473</orcidid></addata></record> |
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| ispartof | Journal of cosmetic dermatology, 2018-12, Vol.17 (6), p.1233-1239 |
| issn | 1473-2130 1473-2165 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Abdomen Adipose Tissue - pathology adipose tissues Adult age Age Factors Aged BMI Body Mass Index Female harvest site Humans Lipectomy Male Middle Aged Pelvis Prospective Studies Sex Factors Stromal Cells stromal vascular fraction Thigh Young Adult |
| title | Effect of age and body mass index on the yield of stromal vascular fraction |
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