Downregulation of miR-335-5p by Long Noncoding RNA ZEB1-AS1 in Gastric Cancer Promotes Tumor Proliferation and Invasion
Recently, long noncoding RNAs (lncRNAs) have emerged as new gene regulators and prognostic biomarkers in several cancers, including gastric cancer (GC). In this study, we investigate the role of lncRNA ZEB1 antisense1 (ZEB1-AS1) on GC progression. In the present study, we found that ZEB1-AS1 express...
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| Veröffentlicht in: | DNA and cell biology 2018-01, Vol.37 (1), p.46-52 |
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| creator | Zhang, Li-Li Zhang, Lan-Fang Guo, Xiao-He Zhang, De-Zhong Yang, Fang Fan, Ying-Ying |
| description | Recently, long noncoding RNAs (lncRNAs) have emerged as new gene regulators and prognostic biomarkers in several cancers, including gastric cancer (GC). In this study, we investigate the role of lncRNA ZEB1 antisense1 (ZEB1-AS1) on GC progression. In the present study, we found that ZEB1-AS1 expression was upregulated in GC tissues and cell lines. High ZEB1-AS1 expression was significantly correlated with advanced TNM stage, lymph node metastasis, and poor overall survival in GC patients. ZEB1-AS1 suppression reduced GC cell proliferation and invasion in vitro. Tumor formation assay in nude mice showed that ZEB1-AS1 inhibition suppressed GC cell growth. Quantitative real-time PCR showed that miR-335-5p expression was downregulated and negatively correlated with ZEB1-AS1 expression in GC tissues. And miR-335-5p expression was directly regulated by ZEB1-AS1. Furthermore, we found that inhibition of miR-335-5p abrogated the suppression of proliferation and invasion of GC cells induced by ZEB1-AS1 depletion. Collectively, ZEB1-AS1 is critical for the proliferation and invasion of GC cells by regulating miR-335-5p. Our findings indicated that ZEB1-AS1 might offer potential novel therapeutic targets for GC patients. |
| doi_str_mv | 10.1089/dna.2017.3926 |
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In this study, we investigate the role of lncRNA ZEB1 antisense1 (ZEB1-AS1) on GC progression. In the present study, we found that ZEB1-AS1 expression was upregulated in GC tissues and cell lines. High ZEB1-AS1 expression was significantly correlated with advanced TNM stage, lymph node metastasis, and poor overall survival in GC patients. ZEB1-AS1 suppression reduced GC cell proliferation and invasion in vitro. Tumor formation assay in nude mice showed that ZEB1-AS1 inhibition suppressed GC cell growth. Quantitative real-time PCR showed that miR-335-5p expression was downregulated and negatively correlated with ZEB1-AS1 expression in GC tissues. And miR-335-5p expression was directly regulated by ZEB1-AS1. Furthermore, we found that inhibition of miR-335-5p abrogated the suppression of proliferation and invasion of GC cells induced by ZEB1-AS1 depletion. Collectively, ZEB1-AS1 is critical for the proliferation and invasion of GC cells by regulating miR-335-5p. Our findings indicated that ZEB1-AS1 might offer potential novel therapeutic targets for GC patients.</description><identifier>ISSN: 1044-5498</identifier><identifier>EISSN: 1557-7430</identifier><identifier>DOI: 10.1089/dna.2017.3926</identifier><identifier>PMID: 29215918</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animal tissues ; Biomarkers ; Biomarkers, Tumor - genetics ; Cancer ; Cell growth ; Cell Line, Tumor ; Cell Movement - genetics ; Cell proliferation ; Cell Proliferation - genetics ; Down-Regulation - genetics ; Gastric cancer ; Gene Expression Regulation, Neoplastic - genetics ; Humans ; Inhibition ; Lymph nodes ; Lymphatic Metastasis - genetics ; Lymphatic Metastasis - pathology ; Metastases ; MicroRNAs - genetics ; Neoplasm Invasiveness - genetics ; Neoplasm Invasiveness - pathology ; Patients ; Regulators ; Ribonucleic acid ; RNA ; RNA, Long Noncoding - genetics ; Stomach Neoplasms - genetics ; Stomach Neoplasms - pathology ; Up-Regulation - genetics ; Zinc Finger E-box-Binding Homeobox 1 - genetics</subject><ispartof>DNA and cell biology, 2018-01, Vol.37 (1), p.46-52</ispartof><rights>(©) Copyright 2018, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29215918$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Li-Li</creatorcontrib><creatorcontrib>Zhang, Lan-Fang</creatorcontrib><creatorcontrib>Guo, Xiao-He</creatorcontrib><creatorcontrib>Zhang, De-Zhong</creatorcontrib><creatorcontrib>Yang, Fang</creatorcontrib><creatorcontrib>Fan, Ying-Ying</creatorcontrib><title>Downregulation of miR-335-5p by Long Noncoding RNA ZEB1-AS1 in Gastric Cancer Promotes Tumor Proliferation and Invasion</title><title>DNA and cell biology</title><addtitle>DNA Cell Biol</addtitle><description>Recently, long noncoding RNAs (lncRNAs) have emerged as new gene regulators and prognostic biomarkers in several cancers, including gastric cancer (GC). In this study, we investigate the role of lncRNA ZEB1 antisense1 (ZEB1-AS1) on GC progression. In the present study, we found that ZEB1-AS1 expression was upregulated in GC tissues and cell lines. High ZEB1-AS1 expression was significantly correlated with advanced TNM stage, lymph node metastasis, and poor overall survival in GC patients. ZEB1-AS1 suppression reduced GC cell proliferation and invasion in vitro. Tumor formation assay in nude mice showed that ZEB1-AS1 inhibition suppressed GC cell growth. Quantitative real-time PCR showed that miR-335-5p expression was downregulated and negatively correlated with ZEB1-AS1 expression in GC tissues. And miR-335-5p expression was directly regulated by ZEB1-AS1. Furthermore, we found that inhibition of miR-335-5p abrogated the suppression of proliferation and invasion of GC cells induced by ZEB1-AS1 depletion. Collectively, ZEB1-AS1 is critical for the proliferation and invasion of GC cells by regulating miR-335-5p. Our findings indicated that ZEB1-AS1 might offer potential novel therapeutic targets for GC patients.</description><subject>Animal tissues</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Cancer</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - genetics</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - genetics</subject><subject>Down-Regulation - genetics</subject><subject>Gastric cancer</subject><subject>Gene Expression Regulation, Neoplastic - genetics</subject><subject>Humans</subject><subject>Inhibition</subject><subject>Lymph nodes</subject><subject>Lymphatic Metastasis - genetics</subject><subject>Lymphatic Metastasis - pathology</subject><subject>Metastases</subject><subject>MicroRNAs - genetics</subject><subject>Neoplasm Invasiveness - genetics</subject><subject>Neoplasm Invasiveness - pathology</subject><subject>Patients</subject><subject>Regulators</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Long Noncoding - genetics</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - pathology</subject><subject>Up-Regulation - genetics</subject><subject>Zinc Finger E-box-Binding Homeobox 1 - genetics</subject><issn>1044-5498</issn><issn>1557-7430</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkM1LwzAAxYMobk6PXiXgxUtnPpv2OOecwpgy58VLSdNkdLRJTVrH_nuLmxdP7z348Xg8AK4xGmOUpPeFlWOCsBjTlMQnYIg5F5FgFJ32HjEWcZYmA3ARwhYhxAlG52BAUoJ5ipMh2D26nfV601WyLZ2FzsC6XEWU8og3MN_DhbMbuHRWuaLs3Wo5gZ-zBxxN3jEsLZzL0PpSwam0Snv45l3tWh3guqvdb6xKo_2hW9oCvthvGfpwCc6MrIK-OuoIfDzN1tPnaPE6f5lOFlFDaNpGhGJTEJILY2KpdIyVkTTnSOOcM67jXMSaM63iAiOlCoqQMMJQxgjPeS41HYG7Q2_j3VenQ5vVZVC6qqTVrgsZTgVHOKEp7dHbf-jWdd7263oqEay_LmE9dXOkurzWRdb4spZ-n_1dSn8AAtB2xg</recordid><startdate>201801</startdate><enddate>201801</enddate><creator>Zhang, Li-Li</creator><creator>Zhang, Lan-Fang</creator><creator>Guo, Xiao-He</creator><creator>Zhang, De-Zhong</creator><creator>Yang, Fang</creator><creator>Fan, Ying-Ying</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201801</creationdate><title>Downregulation of miR-335-5p by Long Noncoding RNA ZEB1-AS1 in Gastric Cancer Promotes Tumor Proliferation and Invasion</title><author>Zhang, Li-Li ; Zhang, Lan-Fang ; Guo, Xiao-He ; Zhang, De-Zhong ; Yang, Fang ; Fan, Ying-Ying</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p239t-231fd22b7ff6ace61cfa3b50e1b545e6b76e54ec6d10ccd3007f7f34425b5bae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animal tissues</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Cancer</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - genetics</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - genetics</topic><topic>Down-Regulation - genetics</topic><topic>Gastric cancer</topic><topic>Gene Expression Regulation, Neoplastic - genetics</topic><topic>Humans</topic><topic>Inhibition</topic><topic>Lymph nodes</topic><topic>Lymphatic Metastasis - genetics</topic><topic>Lymphatic Metastasis - pathology</topic><topic>Metastases</topic><topic>MicroRNAs - genetics</topic><topic>Neoplasm Invasiveness - genetics</topic><topic>Neoplasm Invasiveness - pathology</topic><topic>Patients</topic><topic>Regulators</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Long Noncoding - genetics</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - pathology</topic><topic>Up-Regulation - genetics</topic><topic>Zinc Finger E-box-Binding Homeobox 1 - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Li-Li</creatorcontrib><creatorcontrib>Zhang, Lan-Fang</creatorcontrib><creatorcontrib>Guo, Xiao-He</creatorcontrib><creatorcontrib>Zhang, De-Zhong</creatorcontrib><creatorcontrib>Yang, Fang</creatorcontrib><creatorcontrib>Fan, Ying-Ying</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>DNA and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Li-Li</au><au>Zhang, Lan-Fang</au><au>Guo, Xiao-He</au><au>Zhang, De-Zhong</au><au>Yang, Fang</au><au>Fan, Ying-Ying</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Downregulation of miR-335-5p by Long Noncoding RNA ZEB1-AS1 in Gastric Cancer Promotes Tumor Proliferation and Invasion</atitle><jtitle>DNA and cell biology</jtitle><addtitle>DNA Cell Biol</addtitle><date>2018-01</date><risdate>2018</risdate><volume>37</volume><issue>1</issue><spage>46</spage><epage>52</epage><pages>46-52</pages><issn>1044-5498</issn><eissn>1557-7430</eissn><abstract>Recently, long noncoding RNAs (lncRNAs) have emerged as new gene regulators and prognostic biomarkers in several cancers, including gastric cancer (GC). In this study, we investigate the role of lncRNA ZEB1 antisense1 (ZEB1-AS1) on GC progression. In the present study, we found that ZEB1-AS1 expression was upregulated in GC tissues and cell lines. High ZEB1-AS1 expression was significantly correlated with advanced TNM stage, lymph node metastasis, and poor overall survival in GC patients. ZEB1-AS1 suppression reduced GC cell proliferation and invasion in vitro. Tumor formation assay in nude mice showed that ZEB1-AS1 inhibition suppressed GC cell growth. Quantitative real-time PCR showed that miR-335-5p expression was downregulated and negatively correlated with ZEB1-AS1 expression in GC tissues. And miR-335-5p expression was directly regulated by ZEB1-AS1. Furthermore, we found that inhibition of miR-335-5p abrogated the suppression of proliferation and invasion of GC cells induced by ZEB1-AS1 depletion. Collectively, ZEB1-AS1 is critical for the proliferation and invasion of GC cells by regulating miR-335-5p. 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| subjects | Animal tissues Biomarkers Biomarkers, Tumor - genetics Cancer Cell growth Cell Line, Tumor Cell Movement - genetics Cell proliferation Cell Proliferation - genetics Down-Regulation - genetics Gastric cancer Gene Expression Regulation, Neoplastic - genetics Humans Inhibition Lymph nodes Lymphatic Metastasis - genetics Lymphatic Metastasis - pathology Metastases MicroRNAs - genetics Neoplasm Invasiveness - genetics Neoplasm Invasiveness - pathology Patients Regulators Ribonucleic acid RNA RNA, Long Noncoding - genetics Stomach Neoplasms - genetics Stomach Neoplasms - pathology Up-Regulation - genetics Zinc Finger E-box-Binding Homeobox 1 - genetics |
| title | Downregulation of miR-335-5p by Long Noncoding RNA ZEB1-AS1 in Gastric Cancer Promotes Tumor Proliferation and Invasion |
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