Influence of pharmaceutical residues on the structure of activated sludge bacterial communities in wastewater treatment bioreactors
Concern is growing over contamination of the environment with pharmaceuticals because of their widespread use and incomplete removal during wastewater treatment, where microorganisms drive the key processes. The influence of pharmaceuticals on bacterial community structure in activated sludge was as...
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| Veröffentlicht in: | Water research (Oxford) 2008-11, Vol.42 (17), p.4578-4588 |
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| creator | Kraigher, Barbara Kosjek, Tina Heath, Ester Kompare, Boris Mandic-Mulec, Ines |
| description | Concern is growing over contamination of the environment with pharmaceuticals because of their widespread use and incomplete removal during wastewater treatment, where microorganisms drive the key processes. The influence of pharmaceuticals on bacterial community structure in activated sludge was assessed in small-scale wastewater treatment bioreactors containing different concentrations (5, 50, 200 and 500
μg
L
−1) of several commonly used pharmaceuticals (ibuprofen, naproxen, ketoprofen, diclofenac and clofibric acid). T-RFLP analyses of the bacterial 16S rRNA genes indicated a minor but consistent shift in the bacterial community structure in the bioreactor R50 supplied with pharmaceuticals at a concentration of 50
μg
L
−1, compared to the control reactor R0, which was operated without addition of pharmaceuticals. In the reactors operated with higher concentrations of pharmaceuticals, a greater structural divergence was observed. Bacterial community composition was further investigated by preparation of two clone libraries of bacterial 16S rRNA genes from reactors R0 and R50. Most clones in both libraries belonged to the Betaproteobacteria, among which
Thauera,
Sphaerotilus,
Ideonella and
Acidovorax-related spp. dominated. Nitrite-oxidizing bacteria of the genus
Nitrospira sp., which are key organisms for the second stage of nitrification in wastewater treatment plants, were found only in the clone library of the reactor without pharmaceuticals. In addition, diversity indices were calculated for the two clone libraries, indicating a reduced diversity of activated sludge bacterial community in the reactor supplied with 50
μg
L
−1 of each of selected pharmaceuticals. |
| doi_str_mv | 10.1016/j.watres.2008.08.006 |
| format | Article |
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μg
L
−1) of several commonly used pharmaceuticals (ibuprofen, naproxen, ketoprofen, diclofenac and clofibric acid). T-RFLP analyses of the bacterial 16S rRNA genes indicated a minor but consistent shift in the bacterial community structure in the bioreactor R50 supplied with pharmaceuticals at a concentration of 50
μg
L
−1, compared to the control reactor R0, which was operated without addition of pharmaceuticals. In the reactors operated with higher concentrations of pharmaceuticals, a greater structural divergence was observed. Bacterial community composition was further investigated by preparation of two clone libraries of bacterial 16S rRNA genes from reactors R0 and R50. Most clones in both libraries belonged to the Betaproteobacteria, among which
Thauera,
Sphaerotilus,
Ideonella and
Acidovorax-related spp. dominated. Nitrite-oxidizing bacteria of the genus
Nitrospira sp., which are key organisms for the second stage of nitrification in wastewater treatment plants, were found only in the clone library of the reactor without pharmaceuticals. In addition, diversity indices were calculated for the two clone libraries, indicating a reduced diversity of activated sludge bacterial community in the reactor supplied with 50
μg
L
−1 of each of selected pharmaceuticals.</description><identifier>ISSN: 0043-1354</identifier><identifier>EISSN: 1879-2448</identifier><identifier>DOI: 10.1016/j.watres.2008.08.006</identifier><identifier>PMID: 18786690</identifier><identifier>CODEN: WATRAG</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>16S rRNA ; Acidovorax ; Activated sludge ; Applied sciences ; Bacteria ; Bacteria - classification ; Bacteria - genetics ; Bacteria - metabolism ; Bacterial community structure ; beta-Proteobacteria ; Bioreactors ; clofibric acid ; Cloning, Molecular ; community structure ; Diversity ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; Drug Residues - metabolism ; drugs ; Escherichia coli - genetics ; Escherichia coli - isolation & purification ; Exact sciences and technology ; genes ; Genetic Variation ; ibuprofen ; Ideonella ; nitrification ; nitrites ; Nitrospira ; nonsteroidal anti-inflammatory agents ; Other industrial wastes. Sewage sludge ; Pharmaceuticals ; Phylogeny ; Pollution ; Polymorphism, Restriction Fragment Length ; restriction fragment length polymorphism ; ribosomal RNA ; RNA, Bacterial - genetics ; RNA, Bacterial - isolation & purification ; RNA, Ribosomal, 16S - genetics ; RNA, Ribosomal, 16S - isolation & purification ; Sewage - microbiology ; species diversity ; Sphaerotilus ; T-RFLP ; Thauera ; Waste Disposal, Fluid - methods ; Wastes ; wastewater treatment ; Water Purification - methods ; Water treatment and pollution</subject><ispartof>Water research (Oxford), 2008-11, Vol.42 (17), p.4578-4588</ispartof><rights>2008 Elsevier Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-b687b6c3aa7ca4ac82f7e48c2d0c307ec478279e23edebc462c4fac6c70145713</citedby><cites>FETCH-LOGICAL-c476t-b687b6c3aa7ca4ac82f7e48c2d0c307ec478279e23edebc462c4fac6c70145713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.watres.2008.08.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20810321$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18786690$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kraigher, Barbara</creatorcontrib><creatorcontrib>Kosjek, Tina</creatorcontrib><creatorcontrib>Heath, Ester</creatorcontrib><creatorcontrib>Kompare, Boris</creatorcontrib><creatorcontrib>Mandic-Mulec, Ines</creatorcontrib><title>Influence of pharmaceutical residues on the structure of activated sludge bacterial communities in wastewater treatment bioreactors</title><title>Water research (Oxford)</title><addtitle>Water Res</addtitle><description>Concern is growing over contamination of the environment with pharmaceuticals because of their widespread use and incomplete removal during wastewater treatment, where microorganisms drive the key processes. The influence of pharmaceuticals on bacterial community structure in activated sludge was assessed in small-scale wastewater treatment bioreactors containing different concentrations (5, 50, 200 and 500
μg
L
−1) of several commonly used pharmaceuticals (ibuprofen, naproxen, ketoprofen, diclofenac and clofibric acid). T-RFLP analyses of the bacterial 16S rRNA genes indicated a minor but consistent shift in the bacterial community structure in the bioreactor R50 supplied with pharmaceuticals at a concentration of 50
μg
L
−1, compared to the control reactor R0, which was operated without addition of pharmaceuticals. In the reactors operated with higher concentrations of pharmaceuticals, a greater structural divergence was observed. Bacterial community composition was further investigated by preparation of two clone libraries of bacterial 16S rRNA genes from reactors R0 and R50. Most clones in both libraries belonged to the Betaproteobacteria, among which
Thauera,
Sphaerotilus,
Ideonella and
Acidovorax-related spp. dominated. Nitrite-oxidizing bacteria of the genus
Nitrospira sp., which are key organisms for the second stage of nitrification in wastewater treatment plants, were found only in the clone library of the reactor without pharmaceuticals. In addition, diversity indices were calculated for the two clone libraries, indicating a reduced diversity of activated sludge bacterial community in the reactor supplied with 50
μg
L
−1 of each of selected pharmaceuticals.</description><subject>16S rRNA</subject><subject>Acidovorax</subject><subject>Activated sludge</subject><subject>Applied sciences</subject><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - metabolism</subject><subject>Bacterial community structure</subject><subject>beta-Proteobacteria</subject><subject>Bioreactors</subject><subject>clofibric acid</subject><subject>Cloning, Molecular</subject><subject>community structure</subject><subject>Diversity</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Drug Residues - metabolism</subject><subject>drugs</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - isolation & purification</subject><subject>Exact sciences and technology</subject><subject>genes</subject><subject>Genetic Variation</subject><subject>ibuprofen</subject><subject>Ideonella</subject><subject>nitrification</subject><subject>nitrites</subject><subject>Nitrospira</subject><subject>nonsteroidal anti-inflammatory agents</subject><subject>Other industrial wastes. Sewage sludge</subject><subject>Pharmaceuticals</subject><subject>Phylogeny</subject><subject>Pollution</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>restriction fragment length polymorphism</subject><subject>ribosomal RNA</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Bacterial - isolation & purification</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>RNA, Ribosomal, 16S - isolation & purification</subject><subject>Sewage - microbiology</subject><subject>species diversity</subject><subject>Sphaerotilus</subject><subject>T-RFLP</subject><subject>Thauera</subject><subject>Waste Disposal, Fluid - methods</subject><subject>Wastes</subject><subject>wastewater treatment</subject><subject>Water Purification - methods</subject><subject>Water treatment and pollution</subject><issn>0043-1354</issn><issn>1879-2448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhQtRnHb0H4hmo7tqbx6dpDfCMPgYGHChsw6pW7dm0tSjTVIzuPaPm7Ia3SlcSAjfOZybU1UvOWw5cP3usH3wOVLaCgC7XQb0o2rDrdnXQin7uNoAKFlzuVNn1bOUDgAghNw_rc4KZLXew6b6eTV2_UwjEps6drzzcfBIcw7oe1bcQztTYtPI8h2xlOOMeY6_WY853PtMLUv93N4Sa8oLxVB0OA3DPIYcijSM7MGnTCUsRVYC-zzQmFkTpnLHPMX0vHrS-T7Ri9N5Xt18_PDt8nN9_eXT1eXFdY3K6Fw32ppGo_TeoFceregMKYuiBZRgqFBWmD0JSS01qLRA1XnUaICrneHyvHq7-h7j9L2sld0QElLf-5GmOTm-N8rIMv8FFQhutSqgWkGMU0qROneMYfDxh-Pglpbcwa0tuaUltwzoInt18p-bgdq_olMtBXhzAnwqRXTRjxjSH06A5SDFstHrlev85PxtLMzNVwFcAt9Z0HZJ-H4lqHzsfaDoEoal7jZEwuzaKfw76y-v379y</recordid><startdate>20081101</startdate><enddate>20081101</enddate><creator>Kraigher, Barbara</creator><creator>Kosjek, Tina</creator><creator>Heath, Ester</creator><creator>Kompare, Boris</creator><creator>Mandic-Mulec, Ines</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7QH</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7UA</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H96</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20081101</creationdate><title>Influence of pharmaceutical residues on the structure of activated sludge bacterial communities in wastewater treatment bioreactors</title><author>Kraigher, Barbara ; Kosjek, Tina ; Heath, Ester ; Kompare, Boris ; Mandic-Mulec, Ines</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-b687b6c3aa7ca4ac82f7e48c2d0c307ec478279e23edebc462c4fac6c70145713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>16S rRNA</topic><topic>Acidovorax</topic><topic>Activated sludge</topic><topic>Applied sciences</topic><topic>Bacteria</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - metabolism</topic><topic>Bacterial community structure</topic><topic>beta-Proteobacteria</topic><topic>Bioreactors</topic><topic>clofibric acid</topic><topic>Cloning, Molecular</topic><topic>community structure</topic><topic>Diversity</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Drug Residues - metabolism</topic><topic>drugs</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - isolation & purification</topic><topic>Exact sciences and technology</topic><topic>genes</topic><topic>Genetic Variation</topic><topic>ibuprofen</topic><topic>Ideonella</topic><topic>nitrification</topic><topic>nitrites</topic><topic>Nitrospira</topic><topic>nonsteroidal anti-inflammatory agents</topic><topic>Other industrial wastes. Sewage sludge</topic><topic>Pharmaceuticals</topic><topic>Phylogeny</topic><topic>Pollution</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>restriction fragment length polymorphism</topic><topic>ribosomal RNA</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Bacterial - isolation & purification</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>RNA, Ribosomal, 16S - isolation & purification</topic><topic>Sewage - microbiology</topic><topic>species diversity</topic><topic>Sphaerotilus</topic><topic>T-RFLP</topic><topic>Thauera</topic><topic>Waste Disposal, Fluid - methods</topic><topic>Wastes</topic><topic>wastewater treatment</topic><topic>Water Purification - methods</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kraigher, Barbara</creatorcontrib><creatorcontrib>Kosjek, Tina</creatorcontrib><creatorcontrib>Heath, Ester</creatorcontrib><creatorcontrib>Kompare, Boris</creatorcontrib><creatorcontrib>Mandic-Mulec, Ines</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Water research (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kraigher, Barbara</au><au>Kosjek, Tina</au><au>Heath, Ester</au><au>Kompare, Boris</au><au>Mandic-Mulec, Ines</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of pharmaceutical residues on the structure of activated sludge bacterial communities in wastewater treatment bioreactors</atitle><jtitle>Water research (Oxford)</jtitle><addtitle>Water Res</addtitle><date>2008-11-01</date><risdate>2008</risdate><volume>42</volume><issue>17</issue><spage>4578</spage><epage>4588</epage><pages>4578-4588</pages><issn>0043-1354</issn><eissn>1879-2448</eissn><coden>WATRAG</coden><abstract>Concern is growing over contamination of the environment with pharmaceuticals because of their widespread use and incomplete removal during wastewater treatment, where microorganisms drive the key processes. The influence of pharmaceuticals on bacterial community structure in activated sludge was assessed in small-scale wastewater treatment bioreactors containing different concentrations (5, 50, 200 and 500
μg
L
−1) of several commonly used pharmaceuticals (ibuprofen, naproxen, ketoprofen, diclofenac and clofibric acid). T-RFLP analyses of the bacterial 16S rRNA genes indicated a minor but consistent shift in the bacterial community structure in the bioreactor R50 supplied with pharmaceuticals at a concentration of 50
μg
L
−1, compared to the control reactor R0, which was operated without addition of pharmaceuticals. In the reactors operated with higher concentrations of pharmaceuticals, a greater structural divergence was observed. Bacterial community composition was further investigated by preparation of two clone libraries of bacterial 16S rRNA genes from reactors R0 and R50. Most clones in both libraries belonged to the Betaproteobacteria, among which
Thauera,
Sphaerotilus,
Ideonella and
Acidovorax-related spp. dominated. Nitrite-oxidizing bacteria of the genus
Nitrospira sp., which are key organisms for the second stage of nitrification in wastewater treatment plants, were found only in the clone library of the reactor without pharmaceuticals. In addition, diversity indices were calculated for the two clone libraries, indicating a reduced diversity of activated sludge bacterial community in the reactor supplied with 50
μg
L
−1 of each of selected pharmaceuticals.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>18786690</pmid><doi>10.1016/j.watres.2008.08.006</doi><tpages>11</tpages></addata></record> |
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| ispartof | Water research (Oxford), 2008-11, Vol.42 (17), p.4578-4588 |
| issn | 0043-1354 1879-2448 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 16S rRNA Acidovorax Activated sludge Applied sciences Bacteria Bacteria - classification Bacteria - genetics Bacteria - metabolism Bacterial community structure beta-Proteobacteria Bioreactors clofibric acid Cloning, Molecular community structure Diversity DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Drug Residues - metabolism drugs Escherichia coli - genetics Escherichia coli - isolation & purification Exact sciences and technology genes Genetic Variation ibuprofen Ideonella nitrification nitrites Nitrospira nonsteroidal anti-inflammatory agents Other industrial wastes. Sewage sludge Pharmaceuticals Phylogeny Pollution Polymorphism, Restriction Fragment Length restriction fragment length polymorphism ribosomal RNA RNA, Bacterial - genetics RNA, Bacterial - isolation & purification RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 16S - isolation & purification Sewage - microbiology species diversity Sphaerotilus T-RFLP Thauera Waste Disposal, Fluid - methods Wastes wastewater treatment Water Purification - methods Water treatment and pollution |
| title | Influence of pharmaceutical residues on the structure of activated sludge bacterial communities in wastewater treatment bioreactors |
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