A step-wise approach significantly enhances protein yield of a rationally-designed agonist antibody fragment in E. coli

Fab59 is a rationally-designed antibody fragment (Fab) that mimics the activity of the cytokine thrombopoietin (TPO). Fab59 activity was initially detected directly from bacterial supernatants in a cell-based assay and was subsequently estimated to be equipotent to TPO using purified material. Howev...

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Veröffentlicht in:	Protein expression and purification 2008-05, Vol.59 (1), p.55-63
Hauptverfasser:	Lin, Bing, Renshaw, Mark W., Autote, Kathleen, Smith, Lynette M., Calveley, Peter, Bowdish, Katherine S., Frederickson, Shana
Format:	Artikel
Sprache:	eng
Schlagworte:	Agonist Antibody Cells, Cultured Cloning, Molecular - methods DNA-Binding Proteins - biosynthesis Escherichia coli Escherichia coli - metabolism Escherichia coli Proteins - biosynthesis Genetic Vectors Humans Immunoblotting Immunoglobulin Fab Fragments - biosynthesis Immunoglobulin Fab Fragments - genetics Immunoglobulin Light Chains - biosynthesis Molecular Chaperones - biosynthesis Molecular Mimicry Peptides - metabolism Promoter Regions, Genetic Protein Engineering - methods Receptors, Thrombopoietin - agonists Thrombopoietin - immunology Thrombopoietin - metabolism
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