rapid and cost-effective method of genomic DNA isolation from cyanobacterial culture, mat and soil suitable for genomic fingerprinting and community analysis

This study presents a phenol and lysozyme free protocol for genomic DNA isolation of cyanobacteria from culture, mats and soil. For an efficient and pure DNA isolation from cyanobacteria having tough cell wall, extra steps of glass beading and Sepharose 4B purification were added. The modified metho...

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Veröffentlicht in:	Journal of applied phycology 2007-08, Vol.19 (4), p.373-382
Hauptverfasser:	Srivastava, A. K, Ara, A, Bhargava, P, Mishra, Y, Rai, S. P, Rai, L. C
Format:	Artikel
Sprache:	eng
Schlagworte:	16S rDNA Anabaena Anabaena doliolum Analysis Aulosira fertilissima Axenic culture Beading BLAST Cell culture Cell walls Chloroform Cyanobacteria denaturing gradient gel electrophoresis (DGGE) Deoxyribonucleic acid DNA DNA fingerprinting Fingerprinting Gels Gene sequencing Genetic analysis Genetic fingerprinting Genomics Germfree Hapalosiphon intricatus Lysozyme Methods Nucleotide sequence PCR Phenols Phylogeny Pure culture randomly amplified polymorphic DNA (RAPD) rRNA 16S Soil Soils Tolypothrix tenuis Water purification
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